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QuicKey Human Cys-C(Cystatin C) ELISA Kit

Uniprot : P01034
  • Cat.No.:E-TSEL-H0008

  • Reactivity: Human

To Purchase E-TSEL-H0008

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $390
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Product Details

QuicKey Series

Get more sensitive and precise results with saving at least 1h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 2.5h
Detection range 0.31-20 ng/mL
Sensitivity 0.04 ng/mL
Sample type &Sample volume serum, plasma, urine, saliva; 50μL
Specificity This kit recognizes Human Cys-C in samples. No significant or interference between Human Cys-C and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human Cys-C concentrations in serum, plasma, urine, saliva.Please consult technical support for the applicability if other biological fluids need to be tested.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Cys-C. Samples (or Standards) and biotinylated detection antibody specific for Human Cys-C are added to the micro ELISA plate wells. Human Cys-C would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Cys-C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human Cys-C. You can calculate the concentration of Human Cys-C in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below.
ItemSpecificationsStorage conditions after test
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
2-8℃, 1 month
Reference Standard 96T: 2 vials
48T: 1 vial
Discard unused reconstituted standard and dilutions
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8℃
Biotinylated Detection Ab Working Solution 1 vial, 6 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
2-8℃ (Protect from light)
Substrate Reagent 1 vial, 10 mL
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Technical Data

Sample Values

sandwich-Ab-ELISA-Elabscience

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human Cys-C were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human Cys-C were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.86 2.35 9.01 0.86 2.06 8.64
Standard deviation 0.05 0.11 0.37 0.05 0.10 0.38
CV (%) 5.81 4.68 4.11 5.81 4.85 4.40

Recovery

The recovery of Human Cys-C spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-101 94
EDTA plasma(n=8) 96-108 101
Urine(n=8) 91-105 99

Linearity

Samples were spiked with high concentrations of Human Cys-C and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

sandwich-Ab-ELISA-Elabscience

Target Information

Background

Cystatin C is a non-glycosylated cysteine protease inhibitor with a molecular weight of 13 kDa. It is produced at a constant rate by nucleated cell and freely filtered by the renal glomerulus, and serum cystatin C is an established, novel marker of renal function. Cystatin C is a more specific marker of glomerular filtration rate than creatinine, because levels are independent of age, sex, muscle mass and diet [1]. In addition, high cystatin C is associated with elevated risk of death from myocardial infarction, stroke, and metabolic syndrome. Higher levels of cystatin C and the presence of certain isoforms have also been related to neurological and cerebral disorders such as cerebral amyloid angiopathy, amyotrophic lateral sclerosis, multiple sclerosis, and Alzheimer’s disease. Cystatin isoforms can be detected both in serum and cerebrospinal fluid and more than a dozen have been reported so far.
1. Ferguson T W, Komenda P, Tangri N. Cystatin C as a biomarker for estimating glomerular filtration rate. [J]. Curr Opin Nephrol Hypertens, 2015, 24(3):295-300.
Database LinksSwissProt: P01034
Entrez Gene: 1471
SynonymsCST3, Cystatin-3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin
Research AreaImmunology,Metabolism,Cardiovascular

Assay Procedures

step01

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 90 min at 37°C

step02

2. Aspirate and wash the plate for 3 times

step03

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

step04

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

step05

5. Add 50μL Stop Solution

step06

6. Read the plate at 450nm immediately. Calculation of the results

Citations

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