QuicKey Human IgG(Immunoglobulin G) ELISA Kit

    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
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    >
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience

      Catalog number:E-TSEL-H0011

      Size:
      • 96T
      • 48T
      Qty:
      - +
      Price: $390

      Reactivity: Human

      Detection Range: 1.56~100 ng/mL

      Sensitivity: 0.64 ng/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Product Details

      QuicKey Series

      Get more sensitive and precise results with saving at least 1h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

      Properties

      Assay type Sandwich-ELISA
      Format 96T/48T
      Assay time 2.5 h
      Reactivity Human
      Detection method Colormetric
      Colormetric 1.56-100 ng/mL
      Sensitivity 0.64 ng/mL
      Sample volume 50μL/well
      Sample type serum, plasma, urine, saliva
      Specificity This kit recognizes Human IgG in samples. No significant cross-reactivity or interference between Human IgG and analogues was observed.
      Reproducibility Both intra-CV and inter-CV are < 10%.
      Application This ELISA kit applies to the in vitro quantitative determination of Human IgG concentrations in serum, plasma, urine, saliva. Please consult technical support for the applicability if other biological fluids need to be tested.

      Test principle

      This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IgG. Samples (or Standards) and biotinylated detection antibody specific for Human IgG are added to the micro ELISA plate wells. Human IgG would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human IgG. You can calculate the concentration of Human IgG in the samples by comparing the OD of the samples to the standard curve.

      Kit components & Storage

      An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below. (The specific components might be slightly different in delivered kits, please refer to the manual for more information.)

      Item Specifications Storage conditions after test
      Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips
      48T: 8 wells ×6 strips
      2-8℃, 1 month
      Reference Standard 96T: 2 vials
      48T: 1 vial
      Discard unused reconstituted standard and dilutions
      Reference Standard & Sample Diluent 1 vial, 20 mL 2-8℃
      Biotinylated Detection Ab Working Solution 1 vial, 6 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
      48T: 1 vial, 60 μL
      2-8℃ (Protect from light)
      Substrate Reagent 1 vial, 10 mL
      Stop Solution 1 vial, 10 mL 2-8℃
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution. The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      1. Microplate reader with 450nm wavelength filter

      2. High-precision transfer pipette, EP tubes and disposable pipette tips

      3. Incubator capable of maintaining 37℃

      4. Deionized or distilled water

      5. Absorbent paper

      6. Loading slot for Wash Buffer

      Technical Data

      Sample values

      sandwich-Ab-ELISA-Elabscience

      Typical Standard Curve

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      Concentration(ng/mL) 100 50 25 12.5 6.25 3.13 1.56 0
      OD 2.532 2.078 1.476 1.035 0.635 0.405 0.245 0.111
      Corrected OD 2.421 1.967 1.365 0.924 0.524 0.294 0.134 -
      sandwich-Ab-ELISA-Elabscience

      Precision

      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IgG were tested 20 times on one plate, respectively.

      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IgG were tested on 3 different plates, 20 replicates in each plate.

      Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean(ng/mL) 4.91 11.20 44.60 4.94 11.66 47.68
      Standard deviation 0.26 0.51 1.95 0.25 0.56 2.24
      CV (%) 5.30 4.55 4.37 5.06 4.80 4.70

      Recovery

      The recovery of Human IgG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum(n=8) 87-98 93
      EDTA plasma(n=8) 91-100 95
      Urine(n=8) 83-107 94

      Linearity

      Samples were spiked with high concentrations of Human IgG and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

      Serum(n=4) EDTA plasma (n=4) Urine(n=4)
      1:2 Range (%) 85-96 87-97 86-94
      Average (%) 90 94 91
      1:4 Range (%) 89-98 85-99 88-97
      Average (%) 93 92 91
      1:8 Range (%) 87-102 92-102 94-107
      Average (%) 94 95 99
      1:16 Range (%) 86-98 91-103 86-98
      Average (%) 91 97 90

      Target information

      Background

      Immunoglobulin G (IgG) is a class of immunoglobulins found in all body fluids. They are the smallest but most common antibodies (75 percent to 80 percent) in the body. IgG antibodies are large molecules of about 150 kDa[1], made of four peptide chains. It contains two identical class γ heavy chains of about 50 kDa and two identical light chains of about 25 kDa, thus a tetrameric quaternary structure. IgG antibodies are very important in fighting bacterial and viral infections, and they are the only type of antibody that can cross the placenta in a pregnant woman to help the fetus. The measurement of immunoglobulin G can be a diagnostic tool for certain conditions, such as autoimmune hepatitis, if indicated by certain symptoms. Clinically, measured IgG antibody levels are generally considered to be indicative of an individual's immune status to particular pathogens. A common example of this practice are titers drawn to demonstrate serologic immunity to measles, mumps, and rubella (MMR), hepatitis B virus, and varicella (chickenpox) [2].
      1. Jr C A J, Travers P, Walport M, et al. Principles of innate and adaptive immunity [J]. 2001.
      2. Faruque A A, Maoya J, et al. Analysis of surface plasmon resonance biosensors for protein detection. 2016.

      Research area

      Documents

      Citations

      Please kindly contact us if you have published a paper by citing any products from Elabscience, we will get back to you with the Elabscience Publication Reward ASAP.

      Review more published papers in our Publication Library

      Assay procedures

      step01 1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 90 min at 37°C
      step02 2. Aspirate and wash the plate for 3 times
      step03 3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
      step04 4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
      step05 5. Add 50μL Stop Solution
      step06 6. Read the plate at 450nm immediately. Calculation of the results
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