|Detection range||1.56-100 ng/mL|
|Sample type &Sample volume||serum, plasma, urine, saliva; 50μL|
|Specificity||This kit recognizes Human IgG in samples. No significant or interference between Human IgG and analogues was observed.|
|Reproducibility||Both intra-CV and inter-CV are < 10%.|
|Application||This ELISA kit applies to the in vitro quantitative determination of Human IgG concentrations in serum, plasma, urine, saliva.Please consult technical support for the applicability if other biological fluids need to be tested.|
|Item||Specifications||Storage conditions after test|
|Micro ELISA Plate (Dismountable)||96T: 8 wells ×12 strips48T: 8 wells ×6 strips||2-8℃, 1 month|
|Reference Standard||96T: 2 vials48T: 1 vial||Discard unused reconstituted standard and dilutions|
|Reference Standard & Sample Diluent||1 vial, 20 mL||2-8℃|
|Biotinylated Detection Ab Working Solution||1 vial, 6 mL|
|HRP Conjugate Diluent||1 vial, 14 mL|
|Concentrated Wash Buffer (25×)||1 vial, 30 mL|
|Concentrated HRP Conjugate (100×)||96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
|2-8℃ (Protect from light)|
|Substrate Reagent||1 vial, 10 mL|
|Stop Solution||1 vial, 10 mL||2-8℃|
|Plate Sealer||5 pieces|
|Product Description||1 copy|
|Certificate of Analysis||1 copy|
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IgG were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IgG were tested on 3 different plates, 20 replicates in each plate.
|Intra-assay Precision||Inter-assay Precision|
The recovery of Human IgG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
|Sample Type||Range (%)||Average Recovery (%)|
Samples were spiked with high concentrations of Human IgG and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 90 min at 37°C
2. Aspirate and wash the plate for 3 times
3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
5. Add 50μL Stop Solution
6. Read the plate at 450nm immediately. Calculation of the results
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M*nSubmitted [ Apr 01 2021 ]
Asked: We are using Molecular Device SoftMax Pro 7.1 Software. What settings or protocol design should we perform to get the accurate readings for respective products? Product used by us are: 1. QuicKey Human IgG(Immunoglobulin G) ELISA Kit Cat.No.:E-TSEL-H0011 2. QuicKey Human IgM(Immunoglobulin M) ELISA Kit Cat.No.:E-TSEL-H0010
adminSubmitted [ Apr 01 2021 ]
Answered: Please set four parameter logistic(4PL) curve as the fitting equation to calculate the results, with the standard concentration on the x-axis and the OD values on the y-axis. Our lab applies the Origin as the software to complete it, while Molecular Device SoftMax Pro 7.1 has not been used before, we would provide you with a protocol on how to use Origin, if you'd like to try Origin to calculate results.