QuicKey Human MAU(Microalbuminuria) ELISA Kit

    • ELISA-Elabscience
    • ELISA-Elabscience
    • ELISA-Elabscience
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    • ELISA-Elabscience
    • ELISA-Elabscience
    • ELISA-Elabscience
    • ELISA-Elabscience
    • ELISA-Elabscience
    • ELISA-Elabscience

      Catalog number:E-TSEL-H0005

      Size:
      • 96T
      Qty:
      - +
      Price: $390

      Reactivity: Human

      Detection Range: 1.56~100 ng/mL

      Sensitivity: 0.30 ng/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

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      QuicKey Series

      Get more sensitive and precise results with saving at least 1h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.


      Intended use

      This ELISA kit applies to the in vitro quantitative determination of Human MAU concentrations in urine. Please consult technical support for the applicability if other biological fluids need to be tested.


      Background

      Microalbuminuria is a subtle increase in the urinary excretion of the protein albumin that cannot be detected by a conventional assay. In diabetes, microalbuminuria is an early sign of diabetic kidney disease. Specifically, the excretion of greater than 30 mg and less than 300 mg a day of albumin in the urine. The normal urinary albumin is less than 30 mg per 24 hours and 300 mg or more of urinary albumin per day is considered gross albuminuria. The phenomenon of albuminuria has been recognized for more than 200 years, and its association with kidney disease dates to the epochal insights of Richard Bright in 1827[1]. Microalbuminuria is caused by glomerular capillary injury and so may be a marker for diffuse endothelial dysfunction. According to Steno hypothesis, albuminuria might reflect a general vascular dysfunction and leakage of albumin and other plasma macromolecules such as low density lipoproteins into the vessel wall that may lead to inflammatory responses and in turn start the atherosclerotic process [2].


      1. Glassock R J. Prevention of Microalbuminuria in Type 2 Diabetes: Millimeters or Milligrams? [J]. Journal of the American Society of Nephrology Jasn, 2006, 17(12):3276.

      2. Deckert T, Feldtrasmussen B, Borchjohnsen K, et al. Albuminuria reflects widespread vascular damage [J]. Diabetologia, 1989, 32(4):219.


      Test principle

      This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MAU. Samples (or Standards) and biotinylated detection antibody specific for Human MAU are added to the micro ELISA plate wells. Human MAU would combined with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MAU, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human MAU. You can calculate the concentration of Human MAU in the samples by comparing the OD of the samples to the standard curve.

      Assay type

      Sandwich-ELISA

      Format

      96T

      Assay time

      2.5h

      Reactivity

      Human

      Detection method

      Colormetric

      Detection range

      1.5-100 ng/mL

      Sensitivity

      0.3 ng/mL

      Sample volume

      100μL

      Sample type

      Urine


      Specificity

      This kit recognizes Human MAU in samples. No significant cross-reactivity or interference between MAU and analogues was observed.


      Typical data

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.


      Concentration(ng/mL)

      100

      50

      25

      12.5

      6.25

      3.13

      1.56

      0

      OD

      2.589

      2.006

      1.394

      0.921

      0.534

      0.322

      0.205

      0.079

      Corrected OD

      2.510

      1.927

      1.315

      0.842

      0.455

      0.243

      0.126

      -



      Sample values

      Urine–Samples from apparently healthy volunteers were evaluated for the presence of Human MAU in this assay.

      Sample Type

      Source

      Range

      Dilution Factor

      Urine(n=12)

      Healthy human

      1.53-27.61μg/mL

      50-5000


      Precision

      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human MAU were tested 20 times on one plate, respectively.

      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human MAU were tested on 3 different plates,20 replicates in each plate.

       

      Intra-assay Precision

      Inter-assay Precision

      Sample

      1

      2

      3

      1

      2

      3

      n

      20

      20

      20

      20

      20

      20

      Mean(ng/mL)

      4.02

      12.14

      45.96

      4.33

      12.22

      43.94

      Standard deviation

      0.21

      0.55

      2.48

      0.23

      0.55

      2.18

      CV (%)

      5.22

      4.53

      5.4

      5.31

      4.5

      4.96


      Recovery

      The recovery of Human MAU spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type

      Range (%)

      Average Recovery (%)

      Urine(n=12)

      86-124

      112


      Linearity

      Samples were spiked with high concentrations of Human MAU and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

       

       

      Urine(n=5)

      1:2

      Range (%)

      95-116

      Average (%)

      107

      1:4

      Range (%)

      93-116

      Average (%)

      103

      1:8

      Range (%)

      80-118

      Average (%)

      94

      1:16

      Range (%)

      85-108

      Average (%)

      98


      Kit components & Storage

      An unopened kit can be stored at 2-8 for six months. After test, the unused wells and reagents should be stored according to the table below.

      Item

      Specifications

      Storage conditions after test

      Micro ELISA Plate (Dismountable)

      8 wells ×12 strips

      2-8, 1 months

      Reference Standard

      2 vials

      Discard unused reconstituted standard and dilutions

      Reference Standard & Sample Diluent

      1 vial, 20 mL

      2-8

      Biotinylated Detection Ab Working Solution

      1 vial, 12 mL

      HRP Conjugate Diluent

      1 vial, 14 mL

      Concentrated Wash Buffer (25×)

      1 vial, 30 mL

      Concentrated HRP Conjugate (100×)

      1 vial, 120 μL

      2-8(Protect from light)

      Substrate Reagent

      1 vial, 10 mL

      Stop Solution

      1 vial, 10 mL

      2-8

      Plate Sealer

      5 pieces

       

      Product Description

      1 copy

      Certificate of Analysis

      1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.

      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).


      Other supplies required

      Microplate reader with 450 nm wavelength filter

      High-precision transfer pipette, EP tubes and disposable pipette tips

      37℃ Incubator

      Deionized or distilled water

      Absorbent paper

      Loading slot for Wash Buffer


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