Format	24T, 48T, 96T, 96T5, 96T10
Assay time	2.5h
Sample type	serum, plasma, urine
Sample volume	100μL
Sample size	Run up to 46 samples in duplicate and 30 samples in triplicate
Storage	2-8℃
Interpretation	Detection range: 0.14-100 ng/mL Sensitivity: 0.13 ng/mL
Application	Quantitative determination of Human concentrations in human serum, plasma and other biological fluids
Reproducibility	Both intra-CV and inter-CV are < 10%
Synonyms	TF, PRO1557, PRO2086, TFQTL1, HEL-S-71p, transferrin

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TF. You can calculate the concentration of Human TF in the samples by comparing the OD of the samples to the standard curve.

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Journal of Taibah University Medical Sciences (2022)
Prevalence of anemia in pre-school tribal children with reference to parasitic infections and nutritional impact

DOI: 10.1016/j.jtumed.2022.05.002

Sample: blood

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