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QuicKey Pro Human IGF-1(Insulin-like Growth Factor 1) ELISA Kit

Citations(18)Uniprot : P05019

Cat.No.:E-OSEL-H0012
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T/24
Assay time	1.5h
Detection range	3.12-100 ng/mL
Sensitivity	1.01 ng/mL
Sample type &Sample volume	serum and plasma; 50μL
Specificity	This kit recognizes IGF-1 in samples. No significant cross-reactivity or interference between IGF-1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of IGF-1 concentrations in serum and plasma.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGF-1. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IGF-1 are added to the micro ELISA plate wells. Human IGF-1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody specific to IGF-1. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human IGF-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T	2-8℃, 1 months
Reference Standard	96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials	2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×)	96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL	2-8℃(Protect from light)
Reference Standard & Sample Diluent	96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL	2-8℃
HRP Linked Ab Diluent	96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25×)	96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL
Substrate Reagent	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃(Protect from light)
Stop Solution	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃
Plate Sealer	96T/48T/24T: 5 pieces 96T*5: 25 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level IGF-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level IGF-1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	108.85	204.73	939.71	108.24	188.86	876.39
Standard deviation	6.29	9.87	28.76	6.30	11.05	41.80
CV (%)	5.78	4.82	3.06	5.82	5.85	4.77

Recovery

The recovery of IGF-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	92-103	97
EDTA plasma(n=8)	90-104	95

Linearity

Samples were spiked with high concentrations of Human IGF-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P05019
Synonyms	insulin-like growth factor 1,IGF1
Research Area	Cancer, Developmental biology, Signal transduction

Assay Procedures

Citations

CANCER LETTERS (2021) IF: 8.679
LncCCLM inhibits lymphatic metastasis of cervical cancer by promoting STAU1-mediated IGF-1 mRNA degradation

DOI: 10.1016/j.canlet.2021.07.005

PMID: 34273467
Sample: CC cells
Stem Cells International (2021) IF: 5.443
Mechanical Stress-Induced IGF-1 Facilitates col-I and col-III Synthesis via the IGF-1R/AKT/mTORC1 Signaling Pathway

DOI: 10.1155/2021/5553676

PMID: 34912459
Sample: LFC
Cell Death & Disease (2019) IF: 5.959
Insulin-like growth factor-1 attenuates oxidative stress-induced hepatocyte premature senescence in liver fibrogenesis via regulating nuclear p53–progerin interaction

DOI: 10.1038/s41419-019-1670-6

Sample: plasm
JOURNAL OF AUTISM AND DEVELOPMENTAL DISORDERS (2021) IF: 4.291
Comparison of Serum VEGF, IGF-1, and HIF-1α Levels in Children with Autism Spectrum Disorder and Healthy Controls

DOI: 10.1007/s10803-020-04820-w

PMID: 33389301
Sample: Serum
Scientific Reports (2021) IF: 4.38
Obesity-related proteins score as a potential marker of breast cancer risk

DOI: 10.1038/s41598-021-87583-3

PMID: 33859244
Sample: Plasma
Journal of Hepatocellular Carcinoma (2020) IF: 4.655
Prognostic Significance of Serum Insulin-Like Growth Factor-1 in Hepatocellular Cancer Patients: A Validation Study

DOI: 10.2147/JHC.S258930

Sample: serum
Aging-US (2020) IF: 4.831
MiR-483 induces senescence of human adipose-derived mesenchymal stem cells through IGF1 inhibition

DOI: 10.18632/aging.103818

PMID: 32805717
Sample: Cell sample
JOURNAL OF MOLECULAR ENDOCRINOLOGY (2023) IF: 4.869
CTCF variant begets to short stature by down-regulation of IGF-1

DOI: 10.1530/JME-22-0193

Sample: HEK-293T cell,LO2 cell,immortalized lymphocyte cel
NEUROSCIENCE LETTERS (2022) IF: 3.046
Role of IGF-1 in neuroinflammation and cognition deficits induced by sleep deprivation

DOI: 10.1016/j.neulet.2022.136575

PMID: 35276231
Sample: Plasma
DIABETES RESEARCH AND CLINICAL PRACTICE (2019) IF: 3.239
Ramadan diurnal intermittent fasting modulates SOD2, TFAM, Nrf2, and sirtuins (SIRT1, SIRT3) gene expressions in subjects with overweight and obesity

DOI: 10.1016/j.diabres.2019.107801

Sample: serum
DIABETES RESEARCH AND CLINICAL PRACTICE (2019) IF: 3.239
Effect of Ramadan diurnal fasting on visceral adiposity and serum adipokines in overweight and obese individuals

DOI: 10.1016/j.diabres.2019.05.023

PMID: 31150725
Sample: Serum
Frontiers in Nutrition (2020) IF: 3.365
A Novel 7-Days Prolonged Dietary Deprivation Regimen Improves ALT and UA After 3–6 Months Refeeding, Indicating Therapeutic Potential

DOI: 10.3389/fnut.2020.00050

Sample: serum
GROWTH HORMONE & IGF RESEARCH (2022) IF: 2.372
In missed abortion the decrease of IGF-1 down-regulates PI3K/AKT signaling pathway reducing the secretion of progesterone and β-hCG

DOI: 10.1016/j.ghir.2022.101479

PMID: 35752133
Sample: serum
INVESTIGATIONAL NEW DRUGS (2019) IF: 2.663
The rhenium(I)-diselenoether anticancer drug targets ROS, TGF-β1, VEGF-A, and IGF-1 in an in vitro experimental model of triple-negative breast cancers

DOI: 10.1007/s10637-019-00727-1

PMID: 30632005
Sample: Cell culture medium
ACTA DIABETOLOGICA (2019) IF: 2.996
polymorphisms with diabetic retinopathy in Pakistani population

DOI: 10.1007/s00592-019-01407-5

Sample: Serum
AURIS NASUS LARYNX (2020) IF: 1.436
Association between adenotonsillar hypertrophy and leptin, ghrelin and IGF-1 levels in children

DOI: 10.1016/j.anl.2020.08.002

Sample: serum
Endokrynologia Polska (2021) IF: 1.582
New insights into the metabolic-bone crosstalk in active acromegaly

DOI: 10.5603/EP.a2021.0028

Sample: serum
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES (2016) IF: 0.756
Effect of KiFAY on Performance, Insulin-like Growth Factor-1, and Thyroid Hormones in Broilers

DOI: 10.5713/ajas.16.0188

Sample: Serum

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