Current Position:Home>>Kits>>ELISA Kits>> Rat TRACP-5b(Tartrate Resistant Acid Phosphatase 5b) ELISA Kit

Rat TRACP-5b(Tartrate Resistant Acid Phosphatase 5b) ELISA Kit

Cat.No.:E-EL-R0939
Reactivity: Rat

Filter:

ELISA

To Purchase E-EL-R0939

Size:

96T
48T
24T
96T*5
96T*10

Price: $609

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.63-40 ng/mL
Sensitivity	0.38 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat TRACP-5b in samples. No significant cross-reactivity or interference between Rat TRACP-5b and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat TRACP-5b concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TRACP-5b. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TRACP-5b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TRACP-5b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TRACP-5b. You can calculate the concentration of Rat TRACP-5b in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat TRACP-5b were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat TRACP-5b were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	2.07	5.59	19.86	2.26	5.89	20.98
Standard deviation	0.12	0.24	0.68	0.15	0.24	0.72
CV (%)	5.80	4.29	3.42	6.64	4.07	3.43

Recovery

The recovery of Rat TRACP-5b spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	88-98	93
EDTA plasma (n=8)	93-109	99
Cell culture media (n=8)	84-98	90

Linearity

Samples were spiked with high concentrations of Rat TRACP-5b and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research Area

Cancer, Metabolism, Immunology, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

JOURNAL OF NANOBIOTECHNOLOGY (2021) IF: 10.435
Remote-controllable bone-targeted delivery of estradiol for the treatment of ovariectomy-induced osteoporosis in rats

DOI: 10.1186/s12951-021-00976-4

PMID: 34407835
Sample: serum
LIFE SCIENCES (2022) IF: 5.037
Hyperbaric oxygen therapy improves age induced bone dyshomeostasis in non-obese and obese conditions

DOI: 10.1016/j.lfs.2022.120406

PMID: 35182555
Sample: serum
Frontiers in Endocrinology (2021) IF: 5.555
Chondroitin Sulfate Alleviates Diabetic Osteoporosis and Repairs Bone Microstructure via Anti-Oxidation, Anti-Inflammation, and Regulating Bone Metabolism

DOI: 10.3389/fendo.2021.759843

PMID: 34777254
Sample: serum
BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
Cycloastragenol prevents age-related bone loss: Evidence in d-galactose-treated and aged rats

DOI: 10.1016/j.biopha.2020.110304

PMID: 32497865
Sample: Serum
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020) IF: 4.556
The Administration of 4-Hexylresorcinol Accelerates Orthodontic Tooth Movement and Increases the Expression Level of Bone Turnover Markers in Ovariectomized Rats

DOI: 10.3390/ijms21041526

Sample: blood
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY (2015) IF: 3.628
Osteoprotective effects of estrogen membrane receptor GPR30 in ovariectomized rats

DOI: 10.1016/j.jsbmb.2015.07.002

Sample: Serum
ENDOCRINE (2022) IF: 3.925
Roxadustat improves renal osteodystrophy by dual regulation of bone remodeling

DOI: 10.1007/s12020-022-03199-1

PMID: 36184719
Sample: serum
Acupuncture in Medicine (2018) IF: 2.275
Long-Term Electroacupuncture Stimulation Prevents Osteoporosis in Ovariectomised Osteopaenic Rats through Multiple Signalling Pathways:

DOI: 10.1136/acupmed-2016-011268

Sample: serum
Journal of Orthopaedic Surgery and Research (2022) IF: 2.359
Roxadustat promotes osteoblast differentiation and prevents estrogen deficiency-induced bone loss by stabilizing HIF-1α and activating the Wnt/β-catenin signaling pathway

DOI: 10.1186/s13018-022-03162-w

PMID: 35597989
Sample: serum
BIOSCIENCE REPORTS (2015) IF: 2.637
Osteoprotective effect of combination therapy of low-dose oestradiol with G15, a specific antagonist of GPR30/GPER in ovariectomy-induced osteoporotic rats

DOI: 10.1042/BSR20150146

Sample: Serum
Experimental and Therapeutic Medicine (2015) IF: 1.269
Electroacupuncture at the governor vessel and bladder meridian acupoints improves postmenopausal osteoporosis through osteoprotegerin/RANKL/RANK and Wnt/β‑catenin signaling pathways

DOI: 10.3892/etm.2015.2553

Sample: Serum
Advances in Traditional Medicine (2019)
Ameliorative potential of Saudi Arabian date fruit (Phoenix dactylifera L.) varieties against Freund’s complete adjuvant induced arthritis in rats

DOI: 10.1007/s13596-019-00377-x

Sample: serum

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha