Rat CORT(Corticosterone) ELISA Kit

    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience

      Catalog number:E-EL-R0269


      • 96T
      • 24T
      - +
      Price: $609

      Reactivity: Rat

      Detection Range: 3.13~200 ng/mL

      Sensitivity: 1.88 ng/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

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      Intended use

      This ELISA kit applies to the in vitro quantitative determination of Rat CORT concentrations in serum, plasma and other biological fluids.

      Test principle

      This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat CORT. During the reaction, Rat CORT in the sample or standard competes with a fixed amount of Rat CORT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat CORT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat CORT in the samples is then determined by comparing the OD of the samples to the standard curve.

      Assay type Competitive
      Format 96T
      Assay time 2.0h
      Reactivity Rat
      Detection Method Colormetric
      Detection Range 3.13—200 ng/mL
      Sensitivity 1.88 ng/mL
      Sample Volume 50μL
      Sample Type Serum, plasma and other biological fluids


      This kit recognizes Rat CORT in samples. No significant cross-reactivity or interference between Rat CORT and analogues was observed.

      Typical data

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      Concentration(ng/mL) O.D Average
      200 0.278
      100 0.439
      50 0.637
      25 0.892
      12.5 1.147
      6.25 1.484
      3.13 1.83
      0 2.364


      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat CORT were tested 20 times on one plate, respectively.
      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat CORT were tested on 3 different plates, 20 replicates in each plate.

        Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean (ng/mL) 9.90 24.98 83.60 9.73 22.08 94.15
      Standard deviation 0.54 1.22 3.82 0.49 1.05 3.90
      C V (%) 5.45 4.88 4.57 5.04 4.76 4.14


      The recovery of Rat CORT spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum (n=5) 88-100 93
      EDTA plasma (n=5) 87-102 95
      Cell culture media (n=5) 93-108 100


      Samples were spiked with high concentrations of Rat CORT and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

          Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
      1:2 Range (%) 97-113 90-100 86-99
      Average (%) 105 95 92
      1:4 Range (%) 90-99 92-104 88-101
      Average (%) 95 98 93
      1:8 Range (%) 96-109 91-101 98-107
      Average (%) 102 95 104
      1:16 Range (%) 101-109 83-94 88-99
      Average (%) 105 91 92

      Kit components & Storage

      An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

      Item Specifications Storage
      Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20℃, 6 months
      Reference Standard 2 vials
      Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
      Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20℃(shading light), 6 months
      Reference Standard & Sample Diluent 1 vial, 20 mL 2-8℃, 6 months
      Biotinylated Detection Ab Diluent 1 vial, 14 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Substrate Reagent 1 vial, 10 mL 2-8℃(shading light)
      Stop Solution 1 vial, 10 mL 2-8℃
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      • Microplate reader with 450 nm wavelength filter
      • High-precision transfer pipette, EP tubes and disposable pipette tips
      • Incubator capable of maintaining 37℃
      • Deionized or distilled water
      • Absorbent paper
      • Loading slot for Wash Buffer

      Assay Procedure

      elisa assay procedure 1

      1.Add 50 μL standard or sample to each well.

      elisa assay procedure 2

      2.Immediately add 50 μL Biotinylated Detection Ab to each well.

      elisa assay procedure 3

      3.Incubate for 45 min at 37℃. Aspirate and wash 3 times.

      elisa assay procedure 4

      4.Add 100 μL HRP Conjugate to each well. Incubate for 30 min at 37℃. Aspirate and wash 5 times.

      elisa assay procedure 5

      5.Add 90 μL Substrate Reagent. Incubate for 15 min at 37℃.

      elisa assay procedure 6

      6.Add 50 μL Stop Solution.

      elisa assay procedure 7

      7.Read at 450 nm immediately. Calculation of results.


      1. Publication: Xia N, Li J, Wang H, et al. Schisandra Chinensis And Rhodiola Rosea Exert An Anti-Stress Effect On The HPA Axis And Reduce Hypothalamic C-Fos Expression In Rats Subjected To Repeated Stress[J]. Experimental & Therapeutic Medicine, 2016, 11: 353-359.
        Species: Rat
        Sample Type: Serum
      2. Publication: Li H W, Wei C, Mei Y, et al. Nr3C1-Bhlhb2 Axis Dysregulation Is Involved In The Development Of Attention Deficit Hyperactivity[J]. Molecular Neurobiology, 2016.
        Species: Rat
        Sample Type: Plasma
      3. Publication: Wang T, Shi C G, Li X X, et al. Injection Of Oxytocin Into Paraventricular Nucleus Reverses Depressive-like Behaviors In The Postpartum Depression Rat Model[J]. Brain Research Bulletin, 2018, 336: 236-243.
        Species: Rat
        Sample Type: Plasma
      4. Publication: Michael O S, Olatunji L A. Ameliorative Effect Of Nicotine Exposure On Insulin Resistance Is Accompanied By Decreased Cardiac Glycogen Synthase Kinase-3 And Plasminogen Activator Inhibitor-1 During Oral Oestrogen-Progestin Therapy[J]. Archives Of Physiology And Biochemistry, 2017.
        Species: Rat
        Sample Type: Plasma and tissue homogenate
      5. Publication: Cevik O S, Sahin L, Tamer L. Long term treadmill exercise performed to chronic social isolated rats regulate anxiety behavior without improving learning[J]. Life Sciences, 2018, 200(March): 126-133.
        Species: Rat
      6. Publication: Njan A A, Fatigun C O, Alli-Oluwafuyi A M, et al. Effect of Intranasal Insulin on Peripheral Glucose Profile in Dexamethasone-induced Insulin Resistance in Wistar Rats[J]. Beni-Suef University Journal of Basic and Applied Sciences, 2018.
        Species: Rat
        Sample Type: Cells
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