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Rat cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit

Cat.No.:E-EL-R0151
Reactivity: Rat

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat cTnT/TNNT2 in samples. No significant cross-reactivity or interference between Rat cTnT/TNNT2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat cTnT/TNNT2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat cTnT/TNNT2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat cTnT/TNNT2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat cTnT/TNNT2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat cTnT/TNNT2. You can calculate the concentration of Rat cTnT/TNNT2 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat cTnT/TNNT2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat cTnT/TNNT2 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	53.47	114.59	416.54	57.54	117.04	389.47
Standard deviation	2.78	6.38	22.16	3.02	6.31	17.92
CV (%)	5.20	5.57	5.32	5.25	5.39	4.60

Recovery

The recovery of Rat cTnT/TNNT2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	84-97	91
EDTA plasma (n=8)	95-108	100
Cell culture media (n=8)	89-102	95

Linearity

Samples were spiked with high concentrations of Rat cTnT/TNNT2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	CMH2, CMPD2, LVNC6, RCM3, TnTC
Research Area	Cancer, Cardiovascular, Signal transduction, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
Cardioprotective Effects of Aconite in Isoproterenol-Induced Myocardial Infarction in Rats

DOI: 10.1155/2022/1090893

Sample: serum
Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
An Energy Metabolism Study on the Efficacy of Naoxintong Capsules against Myocardial Infarction in a Rat Model

DOI: 10.1155/2022/3712500

PMID: 35915610
Sample: serum
BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 7.419
Chaihujialonggumulitang shows psycho-cardiology therapeutic effect on acute myocardial infarction with comorbid anxiety by the activation of Nrf2/HO-1 pathway and suppression of oxidative stress and apoptosis

DOI: 10.1016/j.biopha.2022.113437

PMID: 36076489
Sample: serum
Computational and Structural Biotechnology Journal (2020) IF: 6.018
Lipid metabolic signatures deviate in sepsis survivors compared to non-survivors

DOI: 10.1016/j.csbj.2020.11.009

PMID: 33304464
Sample: blood
JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 5.195
Hezi inhibits Tiebangchui-induced cardiotoxicity and preserves its anti-rheumatoid arthritis effects by regulating the pharmacokinetics of aconitine and deoxyaconitine

DOI: 10.1016/j.jep.2022.115915

Sample: serum
FASEB JOURNAL (2019) IF: 5.391
Physiological levels of chromogranin A prevent doxorubicin-induced cardiotoxicity without impairing its anticancer activity

DOI: 10.1096/fj.201802707R

Sample: plasma
Frontiers in Oncology (2022) IF: 5.738
Molecular Changes In Cardiac Tissue As A New Marker To Predict Cardiac Dysfunction Induced By Radiotherapy

DOI: 10.3389/fonc.2022.945521

Sample: plasma
Scientific Reports (2021) IF: 4.38
Targeting mitochondrial reactive oxygen species-mediated oxidative stress attenuates nicotine-induced cardiac remodeling and dysfunction

DOI: 10.1038/s41598-021-93234-4

PMID: 34226619
Sample: heart
MOLECULES (2021) IF: 4.412
Comparative Protective Effect of Nigella sativa Oil and Vitis vinifera Seed Oil in an Experimental Model of Isoproterenol-Induced Acute Myocardial Ischemia in Rats

DOI: 10.3390/molecules26113221

PMID: 34072098
Sample: Serum
NUTRITION RESEARCH (2022) IF: 3.315
Inhibition of fat accumulation, lipid dysmetabolism, cardiac inflammation, and improved NO signalling mediate the protective effects of lycopene against cardio-metabolic disorder in obese female rats

DOI: 10.1016/j.nutres.2022.05.009

Sample: serum
Frontiers in Pharmacology (2019) IF: 3.845
Angiotensin II Type I Receptor Antagonism Attenuates Nicotine-Induced Cardiac Remodeling, Dysfunction, and Aggravation of Myocardial Ischemia-Reperfusion Injury in Rats

DOI: 10.3389/fphar.2019.01493

Sample: heart
Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy (2022) IF: 2.01
Carnosic Acid Protects Against Myocardial Infarction by Controlling Oxidative Stress and Inflammation in Rats

DOI: 10.1007/s43450-021-00216-8

Sample: serum
BMC Cardiovascular Disorders (2022) IF: 2.174
Assessing the risk factors for myocardial infarction in diet-induced prediabetes: myocardial tissue changes

DOI: 10.1186/s12872-022-02758-8

PMID: 35918636
Sample: heart tissue
TOXICOLOGY MECHANISMS AND METHODS (2019) IF: 2.276
Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar rats

DOI: 10.1080/15376516.2018.1542474

Sample: heart tissue
BMC Cardiovascular Disorders (2022) IF: 2.298
H3K9me2 regulation of BDNF expression via G9a partakes in the progression of heart failure

DOI: 10.1186/s12872-022-02621-w

PMID: 35439934
Sample: serum
BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY (2020) IF: 2.651
Impact of prolonged nicotine administration on myocardial function and susceptibility to ischaemia‐reperfusion injury in rats

DOI: 10.1111/bcpt.13500

Sample: Plasma
EUROPEAN JOURNAL OF PHARMACOLOGY (2016) IF: 2.73
The protective role of neuregulin-1: A potential therapy for sepsis-induced cardiomyopathy

DOI: 10.1016/j.ejphar.2016.06.042

Sample: Serum
Cardiovascular Toxicology (2018) IF: 2.989
Anti-fibrotic Actions of Roselle Extract in Rat Model of Myocardial Infarction

DOI: 10.1007/s12012-018-9478-7

Sample: plasma
Chinese Journal of Integrative Medicine (2019) IF: 1.445
Danshensu Ameliorates Cardiac Ischaemia Reperfusion Injury through Activating Sirt1/FoxO1/Rab7 Signal Pathway

DOI: 10.1007/s11655-019-3165-9

Sample: heart
Molecular Medicine Reports (2015) IF: 1.554
Candesartan ameliorates acute myocardial infarction in rats through inducible nitric oxide synthase, nuclear factor‑κB, monocyte chemoattractant protein‑1, activator protein‑1 and restoration of heat shock protein 72

DOI: 10.3892/mmr.2015.4432

Sample: Serum

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