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Rat CTXⅡ(Cross Linked C-telopeptide of Type Ⅱ Collagen) ELISA Kit

Cat.No.:E-EL-R2554
Reactivity: Rat

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96T
48T
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96T*5
96T*10

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat CTXⅡ in samples. No significant cross-reactivity or interference between Rat CTXⅡ and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat CTXⅡ concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CTXⅡ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CTXⅡ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CTXⅡ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CTXⅡ. You can calculate the concentration of Rat CTXⅡ in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat CTXⅡ were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat CTXⅡ were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.47	1.19	4.33	0.43	1.31	4.48
Standard deviation	0.03	0.05	0.19	0.02	0.07	0.20
CV (%)	6.38	4.20	4.39	4.65	5.34	4.46

Recovery

The recovery of Rat CTXⅡ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	92-108	98
EDTA plasma (n=8)	87-99	94
Cell culture media (n=8)	91-104	98

Linearity

Samples were spiked with high concentrations of Rat CTXⅡ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research Area

Metabolism

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Immune Network (2022) IF: 6.303
Mitochondrial Transplantation Ameliorates the Development and Progression of Osteoarthritis

DOI: 10.4110/in.2022.22.e14

Sample: serum
Food & Function (2020) IF: 4.171
Inhibition of cartilage degeneration and subchondral bone deterioration by Spinacia oleracea in human mimic of ACLT-induced osteoarthritis

DOI: 10.1039/D0FO01125H

PMID: 32901645
Sample: Serum
Nutrients (2020) IF: 4.546
FlexPro MD®, a Combination of Krill Oil, Astaxanthin and Hyaluronic Acid, Reduces Pain Behavior and Inhibits Inflammatory Response in Monosodium Iodoacetate-Induced Osteoarthritis in Rats

DOI: 10.3390/nu12040956

Sample: serum
PHYTOTHERAPY RESEARCH (2017) IF: 3.092
Scopoletin‐standardized Morinda elliptica leaf extract suppressed inflammation and cartilage degradation to alleviate osteoarthritis: A preclinical study

DOI: 10.1002/ptr.5949

Sample: Serum
INFLAMMOPHARMACOLOGY (2018) IF: 3.304
prevented osteoarthritis cartilage degeneration by attenuating joint inflammation and collagen breakdown in postmenopausal rat model

DOI: 10.1007/s10787-018-0452-6

Sample: urine
INFLAMMOPHARMACOLOGY (2018) IF: 3.304
) protected against osteoarthritis by mitigating inflammation and cartilage degradation: a preclinical study

DOI: 10.1007/s10787-017-0432-2

Sample: serum
PHYTOTHERAPY RESEARCH (2019) IF: 3.766
Vernonia amygdalina inhibited osteoarthritis development by anti‐inflammatory and anticollagenase pathways in cartilage explant and osteoarthritis‐induced rat model

DOI: 10.1002/ptr.6366

Sample: serum
IMMUNOLOGY LETTERS (2018) IF: 2.436
Lactobacillus acidophilus ameliorates pain and cartilage degradation in experimental osteoarthritis

DOI: 10.1016/j.imlet.2018.07.003

Sample: Serum
MENOPAUSE-THE JOURNAL OF THE NORTH AMERICAN MENOPAUSE SOCIETY (2017) IF: 2.733
Mistletoe fig (Ficus deltoidea Jack) leaf extract prevented postmenopausal osteoarthritis by attenuating inflammation and cartilage degradation in rat model

DOI: 10.1097/GME.0000000000000882

Sample: serum
JOURNAL OF FOOD BIOCHEMISTRY (2019) IF: 1.358
Epicatechin and scopoletin rich Morinda citrifolia (Noni) leaf extract supplementation, mitigated Osteoarthritis via anti‐inflammatory, anti‐oxidative, and anti‐protease pathways

DOI: 10.1111/jfbc.12755

Sample: Serum
Experimental and Therapeutic Medicine (2019) IF: 1.448
Protocatechuic acid attenuates anterior cruciate ligament transection‑induced osteoarthritis by suppressing osteoclastogenesis

DOI: 10.3892/etm.2019.8189

Sample: Tissue homogenate
BIOELECTROMAGNETICS (2016) IF: 1.583
Pulsed electromagnetic field at different stages of knee osteoarthritis in rats induced by low‐dose monosodium iodoacetate: Effect on subchondral trabecular bone microarchitecture and cartilage degradation

DOI: 10.1002/bem.22028

Sample: Serum,Urine

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