Current Position:Home>>Kits>>ELISA Kits>> Rat CTXⅠ(Cross Linked C-telopeptide of Type Ⅰ Collagen) ELISA Kit

Rat CTXⅠ(Cross Linked C-telopeptide of Type Ⅰ Collagen) ELISA Kit

Cat.No.:E-EL-R1456
Reactivity: Rat

Filter:

ELISA

To Purchase E-EL-R1456

Size:

96T
48T
24T
96T*5
96T*10

Price: $609

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat CTXⅠ in samples. No significant cross-reactivity or interference between Rat CTXⅠ and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat CTXⅠ concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CTXⅠ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CTXⅠ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CTXⅠ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CTXⅠ. You can calculate the concentration of Rat CTXⅠ in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat CTXⅠ were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat CTXⅠ were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.52	1.41	4.71	0.52	1.40	4.87
Standard deviation	0.03	0.08	0.15	0.03	0.08	0.17
CV (%)	5.77	5.67	3.18	5.77	5.71	3.49

Recovery

The recovery of Rat CTXⅠ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	94-110	102
EDTA plasma (n=8)	92-109	99
Cell culture media (n=8)	92-106	98

Linearity

Samples were spiked with high concentrations of Rat CTXⅠ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	CTX-I, CTX1, ICTP
Research Area	Metabolism, Cardiovascular, Cancer

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

JOURNAL OF NANOBIOTECHNOLOGY (2021) IF: 10.435
Remote-controllable bone-targeted delivery of estradiol for the treatment of ovariectomy-induced osteoporosis in rats

DOI: 10.1186/s12951-021-00976-4

PMID: 34407835
Sample: serum
Frontiers in Endocrinology (2022) IF: 6.055
A novel extraction method enhanced the osteogenic and anti-osteoporosis effect of tea extract without any hepatotoxicity in ovariectomized rats.

DOI: 10.3389/fendo.2022.951800

Sample: serum
BIOCHEMICAL PHARMACOLOGY (2022) IF: 6.1
Pterostilbene-isothiocyanate impedes RANK/TRAF6 interaction to inhibit osteoclastogenesis, promoting osteogenesis in vitro and alleviating glucocorticoid induced osteoporosis in rats

DOI: 10.1016/j.bcp.2022.115284

PMID: 36209841
Sample: serum
Frontiers in Cell and Developmental Biology (2021) IF: 6.684
miR-27a-5p—Abundant Small Extracellular Vesicles Derived From Epimedium-Preconditioned Bone Mesenchymal Stem Cells Stimulate Osteogenesis by Targeting Atg4B-Mediated Autophagy

DOI: 10.3389/fcell.2021.642646

Sample: serum
CELL BIOLOGY AND TOXICOLOGY (2022) IF: 6.691
Urine-derived stem cells-extracellular vesicles ameliorate diabetic osteoporosis through HDAC4/HIF-1α/VEGFA axis by delivering microRNA-26a-5p

DOI: 10.1007/s10565-022-09713-5

PMID: 35554780
Sample: serum
Frontiers in Endocrinology (2021) IF: 5.555
Chondroitin Sulfate Alleviates Diabetic Osteoporosis and Repairs Bone Microstructure via Anti-Oxidation, Anti-Inflammation, and Regulating Bone Metabolism

DOI: 10.3389/fendo.2021.759843

PMID: 34777254
Sample: serum
Nutrients (2022) IF: 5.719
The Brown Algae Ishige sinicola Extract Ameliorates Ovariectomy-Induced Bone Loss in Rats and Suppresses Osteoclastogenesis through Downregulation of NFATc1/c-Fos

DOI: 10.3390/nu14091683

PMID: 35565651
Sample: serum
Frontiers in Pharmacology (2022) IF: 5.988
Traditional Chinese decoction Si Zhi Wan attenuates ovariectomy (OVX)-induced bone loss by inhibiting osteoclastogenesis and promoting apoptosis of mature osteoclasts.

DOI: 10.3389/fphar.2022.983884

Sample: serum
BONE (2020) IF: 4.147
miR300 intervenes Smad3/β-catenin/RunX2 crosstalk for therapy with an alternate function as indicative biomarker in osteoporosis

DOI: 10.1016/j.bone.2020.115603

Sample: serum
BONE (2020) IF: 4.147
Calcium repletion to rats with calcipenic rickets fails to recover bone quality: A calcipenic “memory”

DOI: 10.1016/j.bone.2020.115562

Sample: Urinary
Frontiers in Pharmacology (2017) IF: 4.4
Adenosine Receptor Stimulation Improves Glucocorticoid-Induced Osteoporosis in a Rat Model

DOI: 10.3389/fphar.2017.00558

Sample: Serum
Antioxidants (2019) IF: 4.52
Protective Effects of Melon Extracts on Bone Strength, Mineralization, and Metabolism in Rats with Ovariectomy-Induced Osteoporosis

DOI: 10.3390/antiox8080306

PMID: 31416178
Sample: Plasma
BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
A prebiotic, short-chain fructo-oligosaccharides promotes peak bone mass and maintains bone mass in ovariectomized rats by an osteogenic mechanism

DOI: 10.1016/j.biopha.2020.110448

PMID: 32776872
Sample: Serum
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020) IF: 4.556
The Administration of 4-Hexylresorcinol Accelerates Orthodontic Tooth Movement and Increases the Expression Level of Bone Turnover Markers in Ovariectomized Rats

DOI: 10.3390/ijms21041526

Sample: blood
Frontiers in Physiology (2023) IF: 4.755
Relative comparison of chronic kidney disease-mineral and bone disorder rat models

DOI: 10.3389/fphys.2023.1083725

Sample: Serum
CALCIFIED TISSUE INTERNATIONAL (2018) IF: 3.293
Prevented Bone Loss in Preclinical Osteoporosis/Osteoarthritis Model by Suppressing Inflammation

DOI: 10.1007/s00223-018-0433-1

Sample: Serum
JOURNAL OF ETHNOPHARMACOLOGY (2020) IF: 3.69
QingYan formula extracts protect against postmenopausal osteoporosis in ovariectomized rat model via active ER-dependent MEK/ERK and PI3K/Akt signal pathways

DOI: 10.1016/j.jep.2020.113644

PMID: 33264660
Sample: serum
EXPERIMENTAL BIOLOGY AND MEDICINE (2021) IF: 2.691
Zoledronic acid modulates osteoclast apoptosis through activation of the NF-κB signaling pathway in ovariectomized rats:

DOI: 10.1177/15353702211011052

Sample: Serum
Molecular Medicine Reports (2021) IF: 2.952
Effects of Melandrium firmum Rohrbach on RANKL‑induced osteoclast differentiation and OVX rats

DOI: 10.3892/mmr.2021.12248

Sample: serum
Experimental and Therapeutic Medicine (2019) IF: 1.448
Protocatechuic acid attenuates anterior cruciate ligament transection‑induced osteoarthritis by suppressing osteoclastogenesis

DOI: 10.3892/etm.2019.8189

Sample: Tissue homogenate
BIOELECTROMAGNETICS (2016) IF: 1.583
Pulsed electromagnetic field at different stages of knee osteoarthritis in rats induced by low‐dose monosodium iodoacetate: Effect on subchondral trabecular bone microarchitecture and cartilage degradation

DOI: 10.1002/bem.22028

Sample: Serum,Urine
Current Developments in Nutrition (2020)
Symbiotic Effects of Kefir and Yacon Flour (Smallanthus sonchifolius) on Bone Remodeling in Rats

DOI: 10.1093/cdn/nzaa045_017

Sample: Urine,Feces

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha

DOI: 10.1186/s12951-021-00976-4
PMID: 34407835	Sample: serum

DOI: 10.1016/j.bcp.2022.115284
PMID: 36209841	Sample: serum

DOI: 10.1007/s10565-022-09713-5
PMID: 35554780	Sample: serum

DOI: 10.3389/fendo.2021.759843
PMID: 34777254	Sample: serum

DOI: 10.3390/nu14091683
PMID: 35565651	Sample: serum

DOI: 10.3390/antiox8080306
PMID: 31416178	Sample: Plasma

DOI: 10.1016/j.biopha.2020.110448
PMID: 32776872	Sample: Serum

DOI: 10.1016/j.jep.2020.113644
PMID: 33264660	Sample: serum

DOI: 10.1016/j.bone.2020.115562
	Sample: Urinary

DOI: 10.3390/ijms21041526
	Sample: blood

DOI: 10.3892/etm.2019.8189
	Sample: Tissue homogenate

DOI: 10.1002/bem.22028
	Sample: Serum,Urine

Rat CTXⅠ(Cross Linked C-telopeptide of Type Ⅰ Collagen) ELISA Kit

To Purchase E-EL-R1456

Product Details

Properties

Test Principle

Kit components & Storage

Technical Data

Precision

Recovery

Linearity

Target Information

Assay Procedures

Citations

JOURNAL OF NANOBIOTECHNOLOGY (2021) IF: 10.435

Frontiers in Endocrinology (2022) IF: 6.055

BIOCHEMICAL PHARMACOLOGY (2022) IF: 6.1

Frontiers in Cell and Developmental Biology (2021) IF: 6.684

CELL BIOLOGY AND TOXICOLOGY (2022) IF: 6.691

Frontiers in Endocrinology (2021) IF: 5.555

Nutrients (2022) IF: 5.719

Frontiers in Pharmacology (2022) IF: 5.988

BONE (2020) IF: 4.147

BONE (2020) IF: 4.147

Frontiers in Pharmacology (2017) IF: 4.4

Antioxidants (2019) IF: 4.52

BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020) IF: 4.556

Frontiers in Physiology (2023) IF: 4.755

CALCIFIED TISSUE INTERNATIONAL (2018) IF: 3.293

JOURNAL OF ETHNOPHARMACOLOGY (2020) IF: 3.69

EXPERIMENTAL BIOLOGY AND MEDICINE (2021) IF: 2.691

Molecular Medicine Reports (2021) IF: 2.952

Experimental and Therapeutic Medicine (2019) IF: 1.448

BIOELECTROMAGNETICS (2016) IF: 1.583

Current Developments in Nutrition (2020)

Reviews/Q&A

People Also Bought

About Us

Subscribe Us

United States

Leave Message

Apply for Free Shipping 24T ELISA Kit

My Cart