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Rat ET-1(Endothelin 1) ELISA Kit

Cat.No.:E-EL-R1458
Reactivity: Rat

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96T
48T
24T
96T*5
96T*10

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.78-50 pg/mL
Sensitivity	0.47 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat ET-1 in samples. No significant cross-reactivity or interference between Rat ET-1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat ET-1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ET-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ET-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ET-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ET-1. You can calculate the concentration of Rat ET-1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat ET-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat ET-1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	2.14	5.93	24.08	2.31	5.77	23.01
Standard deviation	0.11	0.27	1.08	0.14	0.29	1.14
CV (%)	5.14	4.55	4.49	6.06	5.03	4.95

Recovery

The recovery of Rat ET-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-105	101
EDTA plasma (n=8)	97-104	101
Cell culture media (n=8)	97-109	103

Linearity

Samples were spiked with high concentrations of Rat ET-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	EDN1, ET1, HDLCQ7, PPET1, Preproendothelin-1
Research Area	Cancer, Cardiovascular, Metabolism, Signaling transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

BIOMEDICINE & PHARMACOTHERAPY (2023) IF: 7.419
Quince extract resists atherosclerosis in rats by down-regulating the EGFR/PI3K/Akt/GSK-3β pathway

DOI: 10.1016/j.biopha.2023.114330

Sample: serum
Scientific Reports (2016) IF: 5.228
Endothelial dysfunction correlates with decompression bubbles in rats

DOI: 10.1038/srep33390

Sample: Serum
Frontiers in Pharmacology (2021) IF: 5.811
Sinapine Thiocyanate Ameliorates Vascular Endothelial Dysfunction in Hypertension by Inhibiting Activation of the NLRP3 Inflammasome

DOI: 10.3389/fphar.2020.620159

PMID: 33633569
Sample: Serum
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2021) IF: 4.101
Apelin‑13 alleviates diabetic nephropathy by enhancing nitric oxide production and suppressing kidney tissue fibrosis

DOI: 10.3892/ijmm.2021.5008

PMID: 34278446
Sample: serum，nephridial
JOURNAL OF ETHNOPHARMACOLOGY (2020) IF: 4.36
Laxative effect and mechanism of Tiantian Capsule on loperamide-induced constipation in rats

DOI: 10.1016/j.jep.2020.113411

PMID: 32980482
Sample: Serum
BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
Semen Brassicae reduces thoracic aortic remodeling, inflammation, and oxidative damage in spontaneously hypertensive rats

DOI: 10.1016/j.biopha.2020.110400

PMID: 32570115
Sample: Serum
NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES (2022) IF: 4.666
Deleterious effect in endothelin receptor–mediated coronary artery smooth muscle contractility in high-salt diet rats

DOI: 10.1016/j.numecd.2022.10.010

Sample: plasma
JOURNAL OF HYPERTENSION (2022) IF: 4.844
Rho kinase inhibition ameliorates vascular remodeling and blood pressure elevations in a rat model of apatinib-induced hypertension

DOI: 10.1097/HJH.0000000000003060

Sample: plasma
PLoS One (2016) IF: 3.057
Effects of the Infusion of 4% or 20% Human Serum Albumin on the Skeletal Muscle Microcirculation in Endotoxemic Rats

DOI: 10.1371/journal.pone.0151005

Sample: Serum
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2020) IF: 3.098
Emerging roles of let‑7d in attenuating pulmonary arterial hypertension via suppression of pulmonary artery endothelial cell autophagy and endothelin synthesis through ATG16L1 downregulation

DOI: 10.3892/ijmm.2020.4567

Sample: lung
Biomed Research International (2019) IF: 3.411
Telmisartan Attenuates Uric Acid-Induced Epithelial-Mesenchymal Transition in Renal Tubular Cells

DOI: 10.1155/2019/3851718

PMID: 30993112
Sample: Cell culture supernatant
Biomed Research International (2017) IF: 3.411
Plumbagin Alleviates Capillarization of Hepatic Sinusoids In Vitro by Downregulating ET-1, VEGF, LN, and Type IV Collagen

DOI: 10.1155/2017/5603216

Sample: Cell culture supernatant
MEDIATORS OF INFLAMMATION (2020) IF: 3.758
High Concentrations of Uric Acid and Angiotensin II Act Additively to Produce Endothelial Injury

DOI: 10.1155/2020/8387654

PMID: 32565731
Sample: Serum,Cell culture medium
JOURNAL OF ALZHEIMERS DISEASE (2020) IF: 3.909
Zibotentan, an Endothelin A Receptor Antagonist, Prevents Amyloid-β-Induced Hypertension and Maintains Cerebral Perfusion

DOI: 10.3233/JAD-190630

Sample: brain
MOLECULAR BIOLOGY REPORTS (2019) IF: 2.107
var kunstleri in spontaneously hypertensive rats: possible involvement of renin–angiotensin–aldosterone system, endothelial function and anti-oxidant system

DOI: 10.1007/s11033-019-04730-w

Sample: serum,urine
CLINICAL HEMORHEOLOGY AND MICROCIRCULATION (2020) IF: 2.375
Chronic intermittent hypoxia vs chronic continuous hypoxia: Effects on vascular endothelial function and myocardial contractility

DOI: 10.3233/CH-190706

Sample: serum
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2020) IF: 2.575
Angiotensin converting enzyme inhibitor potentiates the hypoglycaemic effect of NG-nitro-L-arginine methyl ester (L-NAME) in rats

DOI: 10.1080/13813455.2020.1780263

PMID: 32584611
Sample: Serum
PHARMACEUTICAL BIOLOGY (2020) IF: 2.971
Effect of phenylacetamide isolated from lepidium apetalum on myocardial injury in spontaneously hypertensive rats and its possible mechanism

DOI: 10.1080/13880209.2020.1778043

Sample: myocardial tissue
Iranian Journal of Basic Medical Sciences (2015) IF: 1.228
Is adalimumab protective in ischemia-reperfusion injury in lung?

PMID: 26949496
Sample: lung tissue
CLINICAL AND EXPERIMENTAL HYPERTENSION (2019) IF: 1.522
Standardized ethanol-water extract of Ficus deltoidea Angustifolia reduces blood pressure in spontaneously hypertensive rats

DOI: 10.1080/10641963.2018.1506467

PMID: 30648895
Sample: Serum
Molecular Medicine Reports (2017) IF: 1.692
Oleanolic acid alleviates diabetic rat carotid artery injury through the inhibition of NLRP3 inflammasome signaling pathways

DOI: 10.3892/mmr.2017.7594

PMID: 28944913
Sample: Serum
ARCHIVOS DE BRONCONEUMOLOGIA (2015) IF: 1.823
Protective Effects of Infliximab on Lung Injury Induced by Methotrexate

DOI: 10.1016/j.arbr.2015.05.012

Sample: lung tissue
Reproductive Biology (2020) IF: 1.862
Leptin-induced increase in blood pressure and markers of endothelial activation during pregnancy in Sprague Dawley rats is prevented by resibufogenin, a marinobufagenin antagonist

DOI: 10.1016/j.repbio.2020.03.004

PMID: 32253169
Sample: Serum
Evidence-based Complementary and Alternative Medicine (2019) IF: 1.984
Piper sarmentosum Leaves Aqueous Extract Attenuates Vascular Endothelial Dysfunction in Spontaneously Hypertensive Rats

DOI: 10.1155/2019/7198592

Sample: mesenteric artery
Chinese Herbal Medicines (2019)
Effects and mechanism of total phenols of Magnolia officinalis combined with Maijunan Tablets on blood pressure of spontaneous hypertensive rats

DOI: 10.1016/j.chmed.2018.12.004

Sample: Plasma,Serum,Tissue homogenate
Natural Product Communications (2020) IF: 0.468
Regulation of PI3k-WNK Pathway by Ethyl Acetate Partition Fraction of Gardenia jasminoides var. radicans Makino in SHR, NRK52e Cells, and IMCD3 Cells:

DOI: 10.1177/1934578X20920989

Sample: Serum

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DOI: 10.3389/fphar.2020.620159
PMID: 33633569	Sample: Serum

DOI: 10.3892/ijmm.2021.5008
PMID: 34278446	Sample: serum，nephridial

DOI: 10.1016/j.jep.2020.113411
PMID: 32980482	Sample: Serum

DOI: 10.1016/j.biopha.2020.110400
PMID: 32570115	Sample: Serum

DOI: 10.1155/2019/3851718
PMID: 30993112	Sample: Cell culture supernatant

DOI: 10.1155/2017/5603216
	Sample: Cell culture supernatant

DOI: 10.1155/2020/8387654
PMID: 32565731	Sample: Serum,Cell culture medium

DOI: 10.1080/13813455.2020.1780263
PMID: 32584611	Sample: Serum

DOI: 10.1080/13880209.2020.1778043
	Sample: myocardial tissue

DOI: 10.1080/10641963.2018.1506467
PMID: 30648895	Sample: Serum

DOI: 10.1016/j.biopha.2023.114330
	Sample: serum

DOI: 10.3892/ijmm.2020.4567
	Sample: lung

DOI: 10.3233/JAD-190630
	Sample: brain

DOI: 10.1007/s11033-019-04730-w
	Sample: serum,urine

DOI: 10.3892/mmr.2017.7594
PMID: 28944913	Sample: Serum

DOI: 10.1016/j.repbio.2020.03.004
PMID: 32253169	Sample: Serum

DOI: 10.1016/j.chmed.2018.12.004
	Sample: Plasma,Serum,Tissue homogenate

Rat ET-1(Endothelin 1) ELISA Kit

To Purchase E-EL-R1458

Product Details

Properties

Test Principle

Kit components & Storage

Technical Data

Precision

Recovery

Linearity

Target Information

Assay Procedures

Citations

BIOMEDICINE & PHARMACOTHERAPY (2023) IF: 7.419

Scientific Reports (2016) IF: 5.228

Frontiers in Pharmacology (2021) IF: 5.811

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2021) IF: 4.101

JOURNAL OF ETHNOPHARMACOLOGY (2020) IF: 4.36

BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545

NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES (2022) IF: 4.666

JOURNAL OF HYPERTENSION (2022) IF: 4.844

PLoS One (2016) IF: 3.057

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2020) IF: 3.098

Biomed Research International (2019) IF: 3.411

Biomed Research International (2017) IF: 3.411

MEDIATORS OF INFLAMMATION (2020) IF: 3.758

JOURNAL OF ALZHEIMERS DISEASE (2020) IF: 3.909

MOLECULAR BIOLOGY REPORTS (2019) IF: 2.107

CLINICAL HEMORHEOLOGY AND MICROCIRCULATION (2020) IF: 2.375

ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2020) IF: 2.575

PHARMACEUTICAL BIOLOGY (2020) IF: 2.971

Iranian Journal of Basic Medical Sciences (2015) IF: 1.228

CLINICAL AND EXPERIMENTAL HYPERTENSION (2019) IF: 1.522

Molecular Medicine Reports (2017) IF: 1.692

ARCHIVOS DE BRONCONEUMOLOGIA (2015) IF: 1.823

Reproductive Biology (2020) IF: 1.862

Evidence-based Complementary and Alternative Medicine (2019) IF: 1.984

Chinese Herbal Medicines (2019)

Natural Product Communications (2020) IF: 0.468

Reviews/Q&A

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