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Rat IL-17(Interleukin 17) ELISA Kit

  • Cat.No.:E-EL-R0566

  • Reactivity: Rat

To Purchase E-EL-R0566

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat IL-17 in samples. No significant cross-reactivity or interference between Rat IL-17 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat IL-17 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-17. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-17 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-17, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-17. You can calculate the concentration of Rat IL-17 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IL-17 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IL-17 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 55.20 149.70 486.20 50.50 153.30 468.50
Standard deviation 3.40 7.90 18.00 2.60 8.70 21.60
CV (%) 6.16 5.28 3.70 5.15 5.68 4.61

Recovery

The recovery of Rat IL-17 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-110 101
EDTA plasma (n=8) 97-111 102
Cell culture media (n=8) 93-104 99

Linearity

Samples were spiked with high concentrations of Rat IL-17 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: Q61453
SynonymsIL-17A, IL17, IL17A,CTLA8

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Related Products

Elabscience® provides ancillary reagents required for ELISA kit developments.11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.
Cat.No. Product Name Specifications
E-ELIR-001 ELISA Plate Coating Buffer(1×) 100mL, 1000mL
E-ELIR-002 ELISA Plate Coating Buffer(5×) 100mL, 1000mL
E-ELIR-003 ELISA Plate Blocking Buffer 100mL, 1000mL
E-ELIR-004 Wash Buffer for Sandwich-ELISA(25×) 100mL, 1000mL
E-ELIR-005 Wash Buffer for Competitive-ELISA(25×) 100mL, 1000mL
E-ELIR-006 Stop Solution 100mL, 1000mL
E-ELIR-007 HRP-conjugate Stabilizer 10mL, 100mL, 500mL
E-ELIR-008 HRP-conjugate Diluent 100mL, 1000mL
E-ELIR-009 Biotinylated Antibody Stabilizer 10mL, 100mL, 500mL
E-ELIR-0010 Biotinylated Antibody Diluent 10mL, 100mL, 500mL
E-ELIR-0011 Sample Diluent 10mL, 100mL, 500mL

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Journal of Crohn's & Colitis (2015) IF: 5.813
    Diet Induced Hyperhomocysteinemia Increases Intestinal Inflammation In An Animal Model Of Colitis.

    DOI: 10.1093/ecco-jcc/jjv094

    PMID: 26071411

    Sample: Plasma
  2. Scientific reports (2017) IF: 4.259
    Effect Of Qianghuo Erhuang Decoction On T Regulatory And T Helper 17 Cells In Treatment Of Adjuvant-Induced Arthritis In Rats.

    DOI: 10.1038/s41598-017-17566-w

    PMID: 29222448

    Sample: Serum
  3. Toxins (2018) IF: 3.273
    The Protective Role of Bacillus velezensis A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice.

    DOI: 10.3390/toxins10110449

    PMID: 30384460

    Sample: Serum, Tissue homogenate
  4. Biomedicine & Pharmacotherapy (2018) IF: 3.457
    Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity.

    DOI: 10.1016/j.biopha.2018.07.081

    PMID: 30119204

    Sample: Serum
  5. Phytotherapy Research (2017) IF: 3.092
    Antiinflammation Effects And Mechanisms Study Of Geniposide On Rats With Collagen-Induced Arthritis.

    DOI: 10.1002/ptr.5775

    PMID: 28127805

    Sample: Cell culture supernatant
  6. Toxicology Research (2020) IF: 2.283
    The effects of phenanthrene exposure on Treg and Th17 cells related cytokines in female rats

    DOI: 10.1093/toxres/tfaa030/5837334

    PMID: 99999999

    Sample: Tissue homogenate
  7. Toxicology Research (2020) IF: 2.283
    The effects of phenanthrene exposure on Treg and Th17 cells related cytokines in female rats

    DOI: 10.1093/toxres/tfaa030

    PMID: 32670559

    Sample: Tissue homogenate
  8. Brain and Behavior (2019) IF: 2.091
    Th17 cells and their cytokines serve as potential therapeutic target in experimental autoimmune neuritis

    DOI: 10.1002/brb3.1478

    PMID: 31742934

    Sample: Serum,Tissue homogenate
  9. Journal of cellular biochemistry (2018) IF: 2.959
    Extracts of Coleus forskohlii relieves cough and asthma symptoms via modulating inflammation and the extracellular matrix

    DOI: 10.1002/jcb.28243

    PMID: 30520122

    Sample: Serum,Bronchoalveolar Lavage Fluid
  10. Digestive diseases and sciences (2018) IF: 2.819
    Effect of Homocysteine on the Differentiation of CD4+ T Cells into Th17 Cells.

    DOI: 10.1007/s10620-018-5177-2

    PMID: 29974377

    Sample: Cell culture supernatant
  11. Virus Research (2014) IF: 2.628
    Galectin-9 Ameliorates Respiratory Syncytial Virus-Induced Pulmonary Immunopathology Through Regulating The Balance Between Th17 And Regulatory T Cells.

    DOI: 10.1016/j.virusres.2014.10.011

    PMID: 25451068

    Sample: Tissue homogenate
  12. Int J Recent Sci Res (2015)
    Interleukin-17: a cardiac biomarker in estimation of cardioprotective effects of tacrolimus in doxorubicin-induced cardiotoxicity: animal model study

    DOI: 99999999

    PMID: 99999999

    Sample: Serum
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