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Rat LH(Luteinizing Hormone) ELISA Kit

Citations(121)

  • Cat.No.:E-EL-R0026

  • Reactivity: Rat
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Manual MSDS

To Purchase E-EL-R0026

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 1.56-100 mIU/mL
Sensitivity 0.94 mIU/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat LH in samples. No significant cross-reactivity or interference between Rat LH and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat LH concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat LH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat LH. You can calculate the concentration of Rat LH in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat LH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat LH were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (mIU/mL) 4.70 15.40 40.80 5.00 15.10 38.80
Standard deviation 0.20 0.70 1.70 0.30 0.90 1.60
CV (%) 4.26 4.55 4.17 6.00 5.96 4.12

Recovery

The recovery of Rat LH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-105 99
EDTA plasma (n=8) 93-105 98
Cell culture media (n=8) 90-105 98

Linearity

Samples were spiked with high concentrations of Rat LH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsLutropin, Lutrophin
Research AreaNeuroscience, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Nature Communications (2021) IF: 14.919
    The Goto-Kakizaki rat is a spontaneous prototypical rodent model of polycystic ovary syndrome

    DOI: 10.1038/s41467-021-21308-y

    PMID: 33594056

    		 Sample: Serum
  2. CARBOHYDRATE POLYMERS (2022) IF: 9.381
    Butylated starch alleviates polycystic ovary syndrome by stimulating the secretion of peptide tyrosine-tyrosine and regulating faecal microbiota

    DOI: 10.1016/j.carbpol.2022.119304

    PMID: 35422283

    		 Sample: serum
  3. ENVIRONMENTAL POLLUTION (2022) IF: 9.988
    Acute exposure to microcystins affects hypothalamic-pituitary axes of male rats

    DOI: 10.1016/j.envpol.2022.120843
    Sample: serum
  4. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2022) IF: 7.129
    Arsenic exposure diminishes ovarian follicular reserve and induces abnormal steroidogenesis by DNA methylation

    DOI: 10.1016/j.ecoenv.2022.113816

    PMID: 36068745

    		 Sample: Serum
  5. Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
    Curcumin Inhibits Hyperandrogen-Induced IRE1α-XBP1 Pathway Activation by Activating the PI3K/AKT Signaling in Ovarian Granulosa Cells of PCOS Model Rats

    DOI: 10.1155/2022/2113293
    Sample: serum
  6. BIOLOGICAL RESEARCH (2022) IF: 7.634
    Utility of melatonin in mitigating ionizing radiation-induced testis injury through synergistic interdependence of its biological properties

    DOI: 10.1186/s40659-022-00401-6
    Sample: serum
  7. Antioxidants (2022) IF: 7.675
    Kelulut Honey Ameliorates Oestrus Cycle, Hormonal Profiles, and Oxidative Stress in Letrozole-Induced Polycystic Ovary Syndrome Rats

    DOI: 10.3390/antiox11101879

    PMID: 36290602

    		 Sample: serum
  8. SCIENCE OF THE TOTAL ENVIRONMENT (2021) IF: 7.963
    Effects of acute exposure to microcystins on hypothalamic-pituitary-adrenal (HPA), -gonad (HPG) and -thyroid (HPT) axes of female rats

    DOI: 10.1016/j.scitotenv.2021.145196

    PMID: 34030373

    		 Sample: Serum
  9. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Protective Effects of Epigallocatechin Gallate for Male Sexual Dysfunction in Streptozotocin-Induced Diabetic Rats

    DOI: 10.3390/ijms23179759

    PMID: 36077157

    		 Sample: serum
  10. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2021) IF: 6.291
    Stereological analysis and transcriptome profiling of testicular injury induced by di-(2-ethylhexyl) phthalate in prepubertal rats

    DOI: 10.1016/j.ecoenv.2021.112326

    PMID: 34015638

    		 Sample: Serum
  11. International Journal of Nanomedicine (2021) IF: 6.4
    Development and Evaluation of Curcumin Encapsulated Self-assembled Nanoparticles as Potential Remedial Treatment for PCOS in a Female Rat Model

    DOI: 10.2147/IJN.S302161

    PMID: 34531655

    		 Sample: serum
  12. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53
    Diacerein ameliorates letrozole-induced polycystic ovarian syndrome in rats

    DOI: 10.1016/j.biopha.2022.112870

    PMID: 35367769

    		 Sample: serum
  13. Oxidative Medicine and Cellular Longevity (2021) IF: 6.543
    Curcumin in Combination with Aerobic Exercise Improves Follicular Dysfunction via Inhibition of the Hyperandrogen-Induced IRE1α/XBP1 Endoplasmic Reticulum Stress Pathway in PCOS-Like Rats

    DOI: 10.1155/2021/7382900
    Sample: serum
  14. LIFE SCIENCES (2022) IF: 6.78
    Moxibustion mitigates mitochondrial dysfunction and NLRP3 inflammatory activation in cyclophosphamide-induced premature ovarian insufficiency rats

    DOI: 10.1016/j.lfs.2022.121283
    Sample: serum
  15. Antioxidants (2020) IF: 5.014
    The Modulatory Role of Vitamin C in Boldenone Undecylenate Induced Testicular Oxidative Damage and Androgen Receptor Dysregulation in Adult Male Rats

    DOI: 10.3390/antiox9111053

    PMID: 33126548

    		 Sample: Serum
  16. LIFE SCIENCES (2022) IF: 5.037
    Studies on testicular ultrastructural and hormonal changes in type-2 diabetic rats treated with highly active antiretroviral therapy conjugated silver nanoparticles

    DOI: 10.1016/j.lfs.2022.120498

    PMID: 35341824

    		 Sample: serum
  17. LIFE SCIENCES (2021) IF: 5.037
    Evaluation of maternal high-fat diet and Quercetin-3-O-rutinoside treatment on the reproductive profile of diet naïve male offspring

    DOI: 10.1016/j.lfs.2021.119179

    PMID: 33577849

    		 Sample: Plasma
  18. JOURNAL OF MOLECULAR ENDOCRINOLOGY (2021) IF: 5.098
    Pathological changes and oxidative stress of the HPG axis in hypothyroid rat

    DOI: 10.1530/JME-21-0095

    PMID: 34270447

    		 Sample: serum
  19. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY (2017) IF: 5.104
    Protective Effects of Fluvastatin on Reproductive Function in Obese Male Rats Induced by High-Fat Diet through Enhanced Signaling of mTOR

    DOI: 10.1159/000457881
    Sample: Serum
  20. CHEMOSPHERE (2019) IF: 5.108
    Impact of binary waterborne mixtures of nickel and zinc on hypothalamic-pituitary-testicular axis in rats

    DOI: 10.1016/j.chemosphere.2019.124501

    PMID: 31398612

    		 Sample: Serum
  21. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022) IF: 5.19
    Dietary myricetin assuages atrazine-mediated hypothalamic-pituitary–testicular axis dysfunction in rats

    DOI: 10.1007/s11356-022-23033-5
    Sample: serum
  22. EUROPEAN JOURNAL OF PHARMACOLOGY (2022) IF: 5.195
    Insights into the role of pERK1/2 signaling in post-cerebral ischemia reperfusion sexual dysfunction in rats

    DOI: 10.1016/j.ejphar.2022.175258

    PMID: 36096157

    		 Sample: serum
  23. Reproductive Biology and Endocrinology (2021) IF: 5.211
    Icariin improves testicular dysfunction via enhancing proliferation and inhibiting mitochondria-dependent apoptosis pathway in high-fat diet and streptozotocin-induced diabetic rats

    DOI: 10.1186/s12958-021-00851-9

    PMID: 34753504

    		 Sample: serum
  24. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2021) IF: 5.31
    Oxidized high-density lipoprotein enhances endocrine disorders and ovarian damage in rats

    DOI: 10.1111/jcmm.16197

    PMID: 34346538

    		 Sample: serum
  25. Food & Function (2022) IF: 5.396
    Lactiplantibacillus plantarum CCFM1019 attenuate polycystic ovary syndrome through butyrate dependent gut–brain mechanism

    DOI: 10.1039/D1FO01744F

    PMID: 35044398

    		 Sample: serum
  26. Artificial Cells Nanomedicine and Biotechnology (2021) IF: 5.678
    Effects of vancomycin linoleic acid nanoparticles on male reproductive indices of Sprague–Dawley rats

    DOI: 10.1080/21691401.2021.1968883
    Sample: serum
  27. Aging-US (2021) IF: 5.682
    Paeoniflorin attenuates DHEA-induced polycystic ovary syndrome via inactivation of TGF-β1/Smads signaling pathway in vivo

    DOI: 10.18632/aging.202564

    PMID: 33638949

    		 Sample: Serum
  28. ENVIRONMENTAL POLLUTION (2019) IF: 5.714
    Selenium abates reproductive dysfunction via attenuation of biometal accumulation, oxido-inflammatory stress and caspase-3 activation in male rats exposed to arsenic

    DOI: 10.1016/j.envpol.2019.113079

    PMID: 31473390

    		 Sample: Serum
  29. Frontiers in Pharmacology (2022) IF: 5.811
    Proteomic Study on the Reproductive Toxicity of Tripterygium Glycosides in Rats

    DOI: 10.3389/fphar.2022.888968

    PMID: 35668950

    		 Sample: serum
  30. Frontiers in Pharmacology (2021) IF: 5.811
    Icariin Ameliorates Diabetic Renal Tubulointerstitial Fibrosis by Restoring Autophagy via Regulation of the miR-192-5p/GLP-1R Pathway

    DOI: 10.3389/fphar.2021.720387
    Sample: serum
  31. Frontiers in Pharmacology (2021) IF: 5.811
    Edible Bird’s Nest Supplementation Improves Male Reproductive Parameters of Sprague Dawley Rat

    DOI: 10.3389/fphar.2021.631402
    Sample: serum
  32. Cell Death & Disease (2017) IF: 5.965
    ANP promotes proliferation and inhibits apoptosis of ovarian granulosa cells by NPRA/PGRMC1/EGFR complex and improves ovary functions of PCOS rats

    DOI: 10.1038/cddis.2017.494
    Sample: Serum
  33. BIOCHIMIE (2021) IF: 4.079
    Rescue effect of sodium acetate in diabetes mellitus-associated testicular dysfunction is accompanied by PCSK9 modulation

    DOI: 10.1016/j.biochi.2021.02.004

    PMID: 33581194

    		 Sample: Serum
  34. BIOLOGICAL TRACE ELEMENT RESEARCH (2023) IF: 4.081
    Therapeutic Potential of Selenium Nanoparticles on Letrozole-Induced Polycystic Ovarian Syndrome in Female Wistar Rats

    DOI: 10.1007/s12011-023-03579-2
    Sample: Serum
  35. TOXICOLOGY (2020) IF: 4.099
    Protocatechuic acid modulates reproductive dysfunction linked to furan exposure in rats

    DOI: 10.1016/j.tox.2020.152556

    PMID: 32758512

    		 Sample: Serum,Tissue homogenate
  36. MOLECULAR AND CELLULAR ENDOCRINOLOGY (2021) IF: 4.102
    Intracerebroventricular asprosin administration strongly stimulates hypothalamic-pituitary-testicular axis in rats

    DOI: 10.1016/j.mce.2021.111451

    PMID: 34500042

    		 Sample: serum
  37. Food & Function (2020) IF: 4.171
    Lactic acid bacteria alleviate polycystic ovarian syndrome by regulating sex hormone related gut microbiota

    DOI: 10.1039/C9FO02554E

    PMID: 32441726

    		 Sample: Serum
  38. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2019) IF: 4.183
    The Effect of Schisandra chinensis Baillon on Cross-Talk between Oxidative Stress, Endoplasmic Reticulum Stress, and Mitochondrial Signaling Pathway in Testes of Varicocele-Induced SD Rat

    DOI: 10.3390/ijms20225785
    Sample: serum
  39. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021) IF: 4.223
    Ameliorative effect of fractionated low-dose gamma radiation in combination with ellagic acid on nicotine-induced hormonal changes and testicular toxicity in rats

    DOI: 10.1007/s11356-020-12334-2

    PMID: 33443739

    		 Sample: Serum
  40. Toxin Reviews (2021) IF: 4.266
    Benzo-a-pyrene-induced reproductive toxicity was abated in rats co-treated with taurine

    DOI: 10.1080/15569543.2021.1949617
    Sample: serum
  41. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM (2021) IF: 4.31
    Bee bread mitigates downregulation of steroidogenic genes, decreased spermatogenesis, and epididymal oxidative stress in male rats fed with high-fat diet

    DOI: 10.1152/ajpendo.00093.2021

    PMID: 34229480

    		 Sample: serum,testis
  42. TOXICOLOGY LETTERS (2021) IF: 4.374
    Effects of prenatal chlorocholine chloride exposure on pubertal development and reproduction of male offspring in rats

    DOI: 10.1016/j.toxlet.2021.08.005

    PMID: 34411681

    		 Sample: serum
  43. Scientific Reports (2022) IF: 4.38
    Testicular ultrastructure and hormonal changes following administration of tenofovir disoproxil fumarate-loaded silver nanoparticle in type-2 diabetic rats

    DOI: 10.1038/s41598-022-13321-y

    PMID: 35688844

    		 Sample: serum
  44. Scientific Reports (2021) IF: 4.38
    Antioxidant and cytoprotective effects of Nigella sativa L. seeds on the testis of monosodium glutamate challenged rats

    DOI: 10.1038/s41598-021-92977-4

    PMID: 34188150

    		 Sample: blood
  45. JOURNAL OF ENDOCRINOLOGY (2016) IF: 4.498
    Reproductive alterations in hyperinsulinemic but normoandrogenic MSG obese female rats

    DOI: 10.1530/JOE-15-0453
    Sample: Serum
  46. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020) IF: 4.556
    MOTILIPERM Ameliorates Immobilization Stress-Induced Testicular Dysfunction via Inhibition of Oxidative Stress and Modulation of the Nrf2/HO-1 Pathway in SD Rats

    DOI: 10.3390/ijms21134750
    Sample: serum
  47. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020) IF: 4.556
    Gui-A-Gra Attenuates Testicular Dysfunction in Varicocele-Induced Rats via Oxidative Stress, ER Stress and Mitochondrial Apoptosis Pathway

    DOI: 10.3390/ijms21239231

    PMID: 33287403

    		 Sample: serum
  48. Frontiers in Physiology (2022) IF: 4.566
    Long-Term Wi-Fi Exposure From Pre-Pubertal to Adult Age on the Spermatogonia Proliferation and Protective Effects of Edible Bird’s Nest Supplementation

    DOI: 10.3389/fphys.2022.828578
    Sample: Serum
  49. ENDOCRINOLOGY (2022) IF: 4.736
    Scream sound induced chronic psychological stress results in diminished ovarian reserve in adult female rat

    DOI: 10.1210/endocr/bqac042
    Sample: serum
  50. Journal of Personalized Medicine (2022) IF: 4.945
    A Selective Histamine H4 Receptor Antagonist, JNJ7777120, Role on Glutamate Transporter Activity in Chronic Depression

    DOI: 10.3390/jpm12020246
    Sample: brain
  51. Journal of Clinical Medicine (2022) IF: 4.964
    Moderate Aerobic Exercise Regulates Follicular Dysfunction by Initiating Brain-Derived Neurotrophic Factor (BDNF)-Mediated Anti-Apoptotic Signaling Pathways in Polycystic Ovary Syndrome

    DOI: 10.3390/jcm11195584

    PMID: 36233452

    		 Sample: serum
  52. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2020) IF: 3.056
    The gut-microbiota-testis axis mediated by the activation of the Nrf2 antioxidant pathway is related to prepuberal steroidogenesis disorders induced by di-(2-ethylhexyl) phthalate

    DOI: 10.1007/s11356-020-09854-2

    PMID: 32588312

    		 Sample: Serum,Tissue homogenate
  53. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2020) IF: 3.056
    Synergistic effect of curcumin and chitosan nanoparticles on nano-hydroxyapatite-induced reproductive toxicity in rats

    DOI: 10.1007/s11356-020-11395-7

    PMID: 33141380

    		 Sample: Plasma
  54. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2020) IF: 3.056
    Synergic effects of aerobic exercise and eugenol supplement on germ cell development and testicular tissue structure in chlorpyrifos-treated animal model

    DOI: 10.1007/s11356-020-08222-4
    Sample: serum
  55. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2020) IF: 3.056
    Kolaviron suppresses dysfunctional reproductive axis associated with multi-walled carbon nanotubes exposure in male rats

    DOI: 10.1007/s11356-020-10324-y

    PMID: 32812151

    		 Sample: Serum,Tissue homogenate
  56. Reproductive Sciences (2021) IF: 3.06
    Reproductive Effects of Nicotinamide on Testicular Function and Structure in Old Male Rats: Oxidative, Apoptotic, Hormonal, and Morphological Analyses

    DOI: 10.1007/s43032-021-00647-7

    PMID: 34101148

    		 Sample: testicular
  57. Reproductive Sciences (2021) IF: 3.06
    Human Amnion Membrane-Derived Mesenchymal Stem Cells and Conditioned Medium Can Ameliorate X-Irradiation-Induced Testicular Injury by Reducing Endoplasmic Reticulum Stress and Apoptosis

    DOI: 10.1007/s43032-021-00753-6

    PMID: 34642916

    		 Sample: serum
  58. REPRODUCTIVE TOXICOLOGY (2020) IF: 3.121
    Dose-dependent reproductive toxicity of sodium benzoate in male rats: Inflammation, oxidative stress and apoptosis

    DOI: 10.1016/j.reprotox.2020.08.014

    PMID: 32911041

    		 Sample: Plasma
  59. BRAIN RESEARCH (2018) IF: 3.125
    Intracerebroventricular injection of histamine induces the hypothalamic-pituitary-gonadal axis activation in male rats

    DOI: 10.1016/j.brainres.2018.08.020
    Sample: plasma
  60. REPRODUCTIVE TOXICOLOGY (2021) IF: 3.143
    Repression of HDAC5 by acetate restores hypothalamic-pituitary-ovarian function in type 2 diabetes mellitus

    DOI: 10.1016/j.reprotox.2021.10.008

    PMID: 34656705

    		 Sample: plasma
  61. REPRODUCTIVE TOXICOLOGY (2022) IF: 3.143
    Bisphenol A exposure advances puberty onset by changing Kiss1 expression firstly in arcuate nucleus at juvenile period in female rats

    DOI: 10.1016/j.reprotox.2022.04.005

    PMID: 35429613

    		 Sample: serum
  62. NEUROPEPTIDES (2022) IF: 3.152
    Effects of intracerebroventricular and intravenous administration of Kisspeptin-54 and Gonadotropin-releasing hormone agonist in rats with ovarian hyperstimulation

    DOI: 10.1016/j.npep.2022.102298

    PMID: 36367482

    		 Sample: serum
  63. HUMAN & EXPERIMENTAL TOXICOLOGY (2023) IF: 3.247
    Thymol co-administration abrogates hexachlorobenzene-induced reproductive toxicities in male rats

    DOI: 10.1177/09603271221149201
    Sample: serum
  64. GENERAL AND COMPARATIVE ENDOCRINOLOGY (2022) IF: 3.255
    Role and mechanism of the p-JAK2/p-STAT3 signaling pathway in follicular development in PCOS rats

    DOI: 10.1016/j.ygcen.2022.114138

    PMID: 36202220

    		 Sample: serum
  65. International Journal of Endocrinology (2022) IF: 3.257
    Harmful Consequences of Proton Pump Inhibitors on Male Fertility: An Evidence from Subchronic Toxicity Study of Esomeprazole and Lansoprazole in Wistar Rats

    DOI: 10.1155/2022/4479261

    PMID: 35529080

    		 Sample: serum
  66. CHEMICO-BIOLOGICAL INTERACTIONS (2019) IF: 3.407
    Very low dose spironolactone protects experimentally-induced polycystic ovarian syndrome from insulin-resistant metabolic disturbances by suppressing elevated circulating testosterone

    DOI: 10.1016/j.cbi.2019.108742
    Sample: plasma
  67. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS (2015) IF: 3.412
    Protective effects of metformin on reproductive function in obese male rats induced by high-fat diet

    DOI: 10.1007/s10815-015-0506-2
    Sample: Serum
  68. MICROSCOPY AND MICROANALYSIS (2020) IF: 3.414
    Immunohistomorphometric and Hormonal Analysis of the Pituitary Gonadotropic Cells After Application of the Nandrolone Decanoate and Swimming Training in Adult Male Rats

    DOI: 10.1017/S143192762000166X
    Sample: serum
  69. Cancer Cell International (2019) IF: 3.439
    Cross-talk between ER stress and mitochondrial pathway mediated adriamycin-induced testicular toxicity and DA-9401 modulate adriamycin-induced apoptosis in Sprague–Dawley rats

    DOI: 10.1186/s12935-019-0805-2
    Sample: Serum
  70. LIFE SCIENCES (2019) IF: 3.448
    Protective role of glutamine against cadmium-induced testicular dysfunction in Wistar rats: Involvement of G6PD activity

    DOI: 10.1016/j.lfs.2019.117250

    PMID: 31899225

    		 Sample: Serum
  71. LIFE SCIENCES (2019) IF: 3.448
    Effects of noopept on cognitive functions and pubertal process in rats with diabetes

    DOI: 10.1016/j.lfs.2019.116698

    PMID: 31356906

    		 Sample: Serum,Tissue homogenate
  72. TOXICOLOGY LETTERS (2019) IF: 3.499
    Effects of chlorocholine chloride on pubertal development and reproductive functions in male rats

    DOI: 10.1016/j.toxlet.2019.10.024

    PMID: 31689472

    		 Sample: Serum
  73. TOXICOLOGY LETTERS (2019) IF: 3.499
    Cholestasis-associated reproductive toxicity in male and female rats: The fundamental role of mitochondrial impairment and oxidative stress

    DOI: 10.1016/j.toxlet.2019.09.009
    Sample: serum
  74. European Review for Medical and Pharmacological Sciences (2022) IF: 3.507
    Spirulina therapeutic potentiality in polycystic ovarian syndrome management using DHEA-induced rat model.

    DOI: 10.26355/eurrev_202204_28604
    Sample: serum
  75. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2021) IF: 3.652
    Abatement of the dysfunctional hypothalamic–pituitary–gonadal axis due to ciprofloxacin administration by selenium in male rats

    DOI: 10.1002/jbt.22741
    Sample: Serum
  76. BIOLOGICAL TRACE ELEMENT RESEARCH (2021) IF: 3.738
    Aluminum Chloride–Induced Reproductive Toxicity in Rats: the Protective Role of Zinc Oxide Nanoparticles

    DOI: 10.1007/s12011-021-03010-8
    Sample: serum
  77. FOOD AND CHEMICAL TOXICOLOGY (2019) IF: 3.775
    Cadmium exposure during prenatal development causes testosterone disruption in multigeneration via SF-1 signaling in rats

    DOI: 10.1016/j.fct.2019.110897

    PMID: 31654709

    		 Sample: Serum
  78. PEPTIDES (2023) IF: 3.867
    Investigation of Irisin's Role in Pubertal Onset Physiology in Female Rats

    DOI: 10.1016/j.peptides.2023.170976
    Sample: serum
  79. MOLECULAR AND CELLULAR ENDOCRINOLOGY (2020) IF: 3.871
    MiR-199-3p modulates the onset of puberty in rodents probably by regulating the expression of Kiss1 via the p38 MAPK pathway

    DOI: 10.1016/j.mce.2020.110994
    Sample: serum
  80. REPRODUCTION (2021) IF: 3.906
    CXCL12 and its receptors regulate granulosa cell apoptosis in PCOS rats and human KGN tumor cells

    DOI: 10.1530/REP-20-0451

    PMID: 33258800

    		 Sample: Serum,Cell culture supernatant(ovarian granulosa c
  81. Scientific Reports (2020) IF: 3.998
    Reproductive effects of subchronic exposure to acetamiprid in male rats

    DOI: 10.1038/s41598-020-65887-0

    PMID: 32488017

    		 Sample: Plasma,Tissue homogenate
  82. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2020) IF: 2.05
    Spironolactone reversed hepato-ovarian triglyceride accumulation caused by letrozole-induced polycystic ovarian syndrome: tissue uric acid—a familiar foe

    DOI: 10.1007/s00210-020-01809-1
    Sample: plasma
  83. Evidence-based Complementary and Alternative Medicine (2018) IF: 2.064
    Research on Establishment of Abnormal Phlegmatic Syndrome with Premature Ovarian Failure Rat Model and Effects of Balgham Munziq Treatment

    DOI: 10.1155/2018/3858209
    Sample: serum
  84. TOXICOLOGY MECHANISMS AND METHODS (2020) IF: 2.295
    Cilostazol protects against cyclophosphamide-induced ovarian toxicity in female rats: role of cAMP and HO-1

    DOI: 10.1080/15376516.2020.1774829

    PMID: 32456565

    		 Sample: Serum
  85. International Journal of Endocrinology (2020) IF: 2.299
    Gestational Exposure to Synthetic Steroid Hormones Impaired Sperm Quantity and Quality in Wistar Rats

    DOI: 10.1155/2020/1814867

    PMID: 32148483

    		 Sample: Serum
  86. Reproductive Biology (2021) IF: 2.376
    Intermittent fasting ameliorates di-(2-ethylhexyl) phthalate-induced precocious puberty in female rats: A study of the hypothalamic–pituitary–gonadal axis

    DOI: 10.1016/j.repbio.2021.100513
    Sample: Serum
  87. Andrology (2017) IF: 2.427
    Naringenin attenuates highly active antiretroviral therapy‐induced sperm DNA fragmentations and testicular toxicity in Sprague‐Dawley rats

    DOI: 10.1111/andr.12439
    Sample: Serum
  88. Experimental and Therapeutic Medicine (2021) IF: 2.447
    Direct effect of RFRP‑3 microinjection into the lateral ventricle on the hypothalamic kisspeptin neurons in ovariectomized estrogen‑primed rats

    DOI: 10.3892/etm.2021.10946
    Sample: blood
  89. BMC Complementary and Alternative Medicine (2019) IF: 2.479
    The ameliorative effect of monotropein, astragalin, and spiraeoside on oxidative stress, endoplasmic reticulum stress, and mitochondrial signaling pathway in varicocelized rats

    DOI: 10.1186/s12906-019-2736-9

    PMID: 31771569

    		 Sample: Serum
  90. BMC Complementary and Alternative Medicine (2019) IF: 2.479
    Cannabinoid-deficient Benin republic hemp ( Cannabis sativa L.) improves semen parameters by reducing prolactin and enhancing anti-oxidant status

    DOI: 10.1186/s12906-019-2541-5
    Sample: plasma
  91. TISSUE & CELL (2023) IF: 2.586
    Melatonin effective to reduce the microscopic symptoms of polycystic ovary syndrome-related infertility: An experimental study

    DOI: 10.1016/j.tice.2023.102015
    Sample: serum
  92. Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
    Moxibustion against Cyclophosphamide-Induced Premature Ovarian Failure in Rats through Inhibiting NLRP3-/Caspase-1-/GSDMD-Dependent Pyroptosis

    DOI: 10.1155/2021/8874757

    PMID: 33613687

    		 Sample: Serum
  93. JOURNAL OF FOOD BIOCHEMISTRY (2021) IF: 2.72
    Chlorogenic acid co-administration abates tamoxifen-mediated reproductive toxicities in male rats: An experimental approach

    DOI: 10.1111/jfbc.13615

    PMID: 33491243

    		 Sample: Serum
  94. Andrology (2018) IF: 2.734
    Virgin coconut oil extract mitigates testicular‐induced toxicity of alcohol use in antiretroviral therapy

    DOI: 10.1111/andr.12490
    Sample: Serum
  95. ANDROLOGIA (2021) IF: 2.775
    Achillea millefolium alleviates testicular damage in paclitaxel-intoxicated rats via attenuation of testicular oxido-inflammatory stress and apoptotic responses

    DOI: 10.1111/and.14028

    PMID: 33650701

    		 Sample: Serum
  96. GENERAL AND COMPARATIVE ENDOCRINOLOGY (2021) IF: 2.822
    Intracerebroventricular salusin-β infusion to rats increases hypothalamus-pituitary-testicular axis hormones

    DOI: 10.1016/j.ygcen.2021.113820

    PMID: 34015344

    		 Sample: Serum
  97. Reproductive Biology and Endocrinology (2018) IF: 2.852
    GnRH dysregulation in polycystic ovarian syndrome (PCOS) is a manifestation of an altered neurotransmitter profile

    DOI: 10.1186/s12958-018-0354-x
    Sample: Serum
  98. Andrology (2020) IF: 2.86
    Orlistat attenuates obesity‐induced decline in steroidogenesis and spermatogenesis by up‐regulating steroidogenic genes

    DOI: 10.1111/andr.12824
    Sample: Tissue homogenate
  99. HUMAN & EXPERIMENTAL TOXICOLOGY (2021) IF: 2.903
    Protective effect of pyrrolidine dithiocarbamate against methotrexate-induced testicular damage:

    DOI: 10.1177/09603271211035674
    Sample: serum
  100. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2019) IF: 2.914
    Attenuation potentials of royal jelly against hydroxyurea-induced infertility through inhibiting oxidation and release of pro-inflammatory cytokines in male rats

    DOI: 10.1007/s11356-019-05521-3
    Sample: serum
  101. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2019) IF: 2.928
    Mitochondria-targeted antioxidant therapy for an animal model of PCOS-IR

    DOI: 10.3892/ijmm.2018.3977

    PMID: 30431108

    		 Sample: Serum
  102. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2019) IF: 2.928
    Expression of FKBP52 in the ovaries of PCOS rats

    DOI: 10.3892/ijmm.2018.3998

    PMID: 30483787

    		 Sample: Serum
  103. Molecular Medicine Reports (2016) IF: 2.952
    Curcumin ameliorates high‑fat diet‑induced spermatogenesis dysfunction

    DOI: 10.3892/mmr.2016.5712
    Sample: Serum
  104. African Health Sciences (2022) IF: 1.108
    Regulation of male sex hormones strongly heightens copulation behavior indices in rat’s model administered Cocos nucifera husks extract

    DOI: 10.4314/ahs.v22i2.47
    Sample: serum
  105. Experimental and Therapeutic Medicine (2018) IF: 1.41
    Asiatic acid attenuates high-fat diet-induced impaired spermatogenesis

    DOI: 10.3892/etm.2017.5672
    Sample: serum
  106. ZYGOTE (2021) IF: 1.442
    GPR120 agonist ameliorated insulin resistance and improved ovarian function

    DOI: 10.1017/S0967199421000873

    PMID: 34879886

    		 Sample: serum
  107. ANDROLOGIA (2017) IF: 1.458
    Adjuvant potential of virgin coconut oil extract on antiretroviral therapy‐induced testicular toxicity: An ultrastructural study

    DOI: 10.1111/and.12930
    Sample: serum
  108. AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL (2015) IF: 1.562
    Brain thromboxane A2 via arachidonic acid cascade induces the hypothalamic–pituitary–gonadal axis activation in rats

    DOI: 10.1016/j.autneu.2015.02.005
    Sample: Plasma
  109. ANDROLOGIA (2018) IF: 1.588
    Testicular microanatomical and hormonal alterations following use of antiretroviral therapy in Sprague Dawley rats: Role of Naringenin

    DOI: 10.1111/and.13137

    PMID: 30168163

    		 Sample: Serum
  110. JOURNAL OF FOOD BIOCHEMISTRY (2020) IF: 1.662
    Ferulic acid influences Nrf2 activation to restore testicular tissue from cadmium‐induced oxidative challenge, inflammation, and apoptosis in rats

    DOI: 10.1111/jfbc.13505
    Sample: Serum,Tissue homogenate
  111. REPRODUCTION FERTILITY AND DEVELOPMENT (2020) IF: 1.721
    Opposite effects of high- and low-dose di-(2-ethylhexyl) phthalate (DEHP) exposure on puberty onset, oestrous cycle regularity and hypothalamic kisspeptin expression in female rats

    DOI: 10.1071/RD19024
    Sample: serum
  112. Journal of Clinical Research in Pediatric Endocrinology (2020) IF: 1.803
    Effects of 5-Hydroxymethylfurfural on Pubertal Development of Female Wistar Rats

    DOI: 10.4274/jcrpe.galenos.2019.2019.0080

    PMID: 31475510

    		 Sample: Serum
  113. Evidence-based Complementary and Alternative Medicine (2020) IF: 1.813
    Duzhong Butiansu Prescription Improves Heat Stress-Induced Spermatogenic Dysfunction by Regulating Sperm Formation and Heat Stress Pathway

    DOI: 10.1155/2020/6723204

    PMID: 32184896

    		 Sample: Serum
  114. ANDROLOGIA (2019) IF: 1.84
    Effects of N‐nitro L‐arginine methyl ester and α‐tocopherol on testicular oxidative stress caused by exposure to cigarette smoke

    DOI: 10.1111/and.13355
    Sample: serum
  115. INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH (2020) IF: 1.916
    Effects of prenatal and lactational bisphenol a and/or di(2-ethylhexyl) phthalate exposure on male reproductive system

    DOI: 10.1080/09603123.2020.1805416

    PMID: 32787440

    		 Sample: Plasma
  116. Journal of Experimental Zoology Part A-Ecological and Integrative Physiology (2020) IF: 1.917
    Regulation of the hypothalamic GnRH–GnIH system by putrescine in adult female rats and GT1-7 neuronal cell line

    DOI: 10.1002/jez.2351
    Sample: serum
  117. ANDROLOGIA (2020) IF: 1.951
    Selenium attenuates diclofenac‐induced testicular and epididymal toxicity in rats

    DOI: 10.1111/and.13669
    Sample: Sperm
  118. Cureus (2021)
    The Interplay Between Hyperthyroidism and Ovarian Cytoarchitecture in Albino Rats

    DOI: 10.7759/cureus.14517
    Sample: serum
  119. International Journal of Fertility & Sterility (2017)
    Aluminium-Induced Oxidative Stress, Apoptosis and Alterations in Testicular Tissue and Sperm Quality in Wistar Rats: Ameliorative Effects of Curcumin

    DOI: 10.22074/ijfs.2017.4859
    Sample: plasma
  120. ACUPUNCTURE & ELECTRO-THERAPEUTICS RESEARCH (2021) IF: 0.143
    The Effectiveness of Electro-acupuncture in Ovarian Ischemia Reperfusion Injury

    DOI: 10.3727/036012921X16264625521781
    Sample: serum
  121. Asian Pacific Journal of Tropical Medicine (2017) IF: 0.925
    Rescue effects of aqueous seed extracts of Foeniculum vulgare and Carum carvi against cadmium-induced hepatic, renal and gonadal damage in female albino rats

    DOI: 10.1016/j.apjtm.2017.10.019
    Sample: Serum
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