Rat MAU (Microalbuminuria) CLIA Kit

    • CLIA-Elabscience
    • CLIA-Elabscience
    • CLIA-Elabscience
    <
    >
    • CLIA-Elabscience
    • CLIA-Elabscience
    • CLIA-Elabscience
    • CLIA-Elabscience
    • CLIA-Elabscience
    • CLIA-Elabscience

      Catalog number:E-CL-R0023

      Synonyms:UMA

      Size:
      • 96T
      Qty:
      - +
      Price: $682

      Reactivity: Rat

      Detection Range: 0.31~20 μg/mL

      Sensitivity: 0.19 μg/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Intended use

      This CLIA kit applies to the in vitro quantitative determination of Rat MAU concentrations in serum, plasma and other biological fluids.

      Test principle

      This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MAU. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MAU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MAU, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MAU. You can calculate the concentration of Rat MAU in the samples by comparing the RLU value of the samples to the standard curve.

      Assay type Sandwich
      Format 96T
      Assay time 3.5h
      Reactivity Rat
      Detection method Chemiluminescence
      Detection range 0.31-20 μg/mL
      Sensitivity 0.19 μg/mL
      Sample volume 100μL
      Sample type urine
      Repeatability CV < 15%

      Specificity

      This kit recognizes Rat MAU in samples. No significant cross-reactivity or interference between Rat MAU and analogues was observed.

      Typical data

      As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      Concentration
      (μg/mL)
      RLU Average Corrected
      20 32824
      35140
      33982 33950
      10 11398
      13542
      12470 12438
      5 5411
      5089
      5250 5218
      2.5 2415
      2633
      2524 2492
      1.25 1477
      1289
      1383 1351
      0.63 917
      817
      867 835
      0.31 620
      626
      623 591
      0 32
      32
      32 --

      Precision

      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat MAU were tested 20 times on one plate, respectively.
      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat MAU were tested on 3 different plates, 20 replicates in each plate.

        Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean (μg/mL) 1.06 2.46 7.19 1.08 2.48 7.21
      Standard deviation 0.11 0.18 0.59 0.10 0.20 0.55
      C V (%) 10.38 7.32 8.21 9.26 8.06 7.63

      Recovery

      The recovery of Rat MAU spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum (n=5) 91-107 98
      EDTA plasma (n=5) 98-111 105
      Cell culture media (n=5) 88-102 94

      Linearity

      Samples were spiked with high concentrations of Rat MAU and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

          Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
      1:2 Range (%) 94-106 101-120 89-100
      Average (%) 100 109 94
      1:4 Range (%) 87-101 95-108 93-107
      Average (%) 92 102 99
      1:8 Range (%) 96-111 89-102 94-108
      Average (%) 101 96 101
      1:16 Range (%) 103-115 101-120 93-108
      Average (%) 109 109 100

      Kit components& Storage

      An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

      Item Specifications Storage
      Micro CLIA Plate(Dismountable) 8 wells ×12 strips -20℃, 6 months
      Reference Standard 2 vials
      Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
      Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20℃(shading light), 6 months
      Reference Standard & Sample Diluent 1 vial, 20 mL 2-8℃, 6 months
      Biotinylated Detection Ab Diluent 1 vial, 14 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Substrate Reagent A 1 vial, 5 mL 2-8℃ (shading light)
      Substrate Reagent B 1 vial, 5 mL 2-8℃ (shading light)
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      • Chemiluminescence immunoassay analyzer
      • High-precision transfer pipette, EP tubes and disposable pipette tips
      • Incubator capable of maintaining 37℃
      • Deionized or distilled water
      • Absorbent paper
      • Loading slot for Wash Buffer

      Assay procedure

      elisa assay procedure 1

      1.Add 100 μL of standard or sample to each well. Incubate for 90 min at 37℃.

      elisa assay procedure 2

      2.Remove the liquid.

      elisa assay procedure 3

      3.Add 100 μL Biotinylated Detection Ab. Incubate 1 hour at 37℃. Aspirate and wash 3 times.

      elisa assay procedure 4

      4.Add 100 μL HRP Conjugate. Incubate 30 min at 37℃. Aspirate and wash 5 times.

      elisa assay procedure 5

      5.Add 100 μL Substrate Mixture Solution. Incubate for 5 min at 37℃.

      elisa assay procedure 6

      6.Fluorescence appeared. Measure the RLU value with the Chemiluminescence immunoassay analyzer. Calculation of the results.

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