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Rat OC/BGP(Osteocalcin) ELISA Kit

Citations(37)

  • Cat.No.:E-EL-R0243

  • Reactivity: Rat
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  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.78-50 ng/mL
Sensitivity 0.47 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat OC/BGP in samples. No significant cross-reactivity or interference between Rat OC/BGP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat OC/BGP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat OC/BGP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat OC/BGP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat OC/BGP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat OC/BGP. You can calculate the concentration of Rat OC/BGP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat OC/BGP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat OC/BGP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 2.70 4.70 20.10 2.80 5.10 19.80
Standard deviation 0.10 0.30 1.00 0.20 0.20 0.80
CV (%) 3.70 6.38 4.98 7.14 3.92 4.04

Recovery

The recovery of Rat OC/BGP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-98 91
EDTA plasma (n=8) 86-96 91
Cell culture media (n=8) 95-109 102

Linearity

Samples were spiked with high concentrations of Rat OC/BGP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsBGLAP, OT, Bone Gamma-carboxyglutamate Protein, Bone Gla Protein
Research AreaCancer, Signal transduction, Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. BIOMATERIALS (2022) IF: 15.304
    Immunology and bioinformatics analysis of injectable organic/inorganic microfluidic microspheres for promoting bone repair

    DOI: 10.1016/j.biomaterials.2022.121685

    PMID: 35953327

    		 Sample: BMSCs
  2. JOURNAL OF NANOBIOTECHNOLOGY (2021) IF: 10.435
    Remote-controllable bone-targeted delivery of estradiol for the treatment of ovariectomy-induced osteoporosis in rats

    DOI: 10.1186/s12951-021-00976-4

    PMID: 34407835

    		 Sample: serum
  3. Frontiers in Endocrinology (2022) IF: 6.055
    Investigating the Effects of Diet-Induced Pre-Diabetes on the Functioning of Calcium-Regulating Organs in Male Sprague Dawley Rats: Effects on Selected Markers

    DOI: 10.3389/fendo.2022.914189

    PMID: 35898447

    		 Sample: plasma
  4. Frontiers in Cell and Developmental Biology (2021) IF: 6.684
    miR-27a-5p—Abundant Small Extracellular Vesicles Derived From Epimedium-Preconditioned Bone Mesenchymal Stem Cells Stimulate Osteogenesis by Targeting Atg4B-Mediated Autophagy

    DOI: 10.3389/fcell.2021.642646
    Sample: serum
  5. CELL BIOLOGY AND TOXICOLOGY (2022) IF: 6.691
    Urine-derived stem cells-extracellular vesicles ameliorate diabetic osteoporosis through HDAC4/HIF-1α/VEGFA axis by delivering microRNA-26a-5p

    DOI: 10.1007/s10565-022-09713-5

    PMID: 35554780

    		 Sample: serum
  6. Frontiers in Molecular Biosciences (2021) IF: 5.246
    miR-330–5p in Small Extracellular Vesicles Derived From Plastrum testudinis-Preconditioned Bone Mesenchymal Stem Cells Attenuates Osteogenesis by Modulating Wnt/β-Catenin Signaling

    DOI: 10.3389/fmolb.2021.679345
    Sample: serum
  7. Nanomedicine (2022) IF: 5.307
    Selenium nanoparticles stimulate osteoblast differentiation via BMP-2/MAPKs/β-catenin pathway in diabetic osteoporosis

    DOI: 10.2217/nnm-2021-0401

    PMID: 35465693

    		 Sample: serum
  8. TALANTA (2020) IF: 5.339
    Aptamer selection and aptasensor construction for bone density biomarkers

    DOI: 10.1016/j.talanta.2020.121818

    PMID: 33379043

    		 Sample: serum
  9. Frontiers in Endocrinology (2021) IF: 5.555
    Chondroitin Sulfate Alleviates Diabetic Osteoporosis and Repairs Bone Microstructure via Anti-Oxidation, Anti-Inflammation, and Regulating Bone Metabolism

    DOI: 10.3389/fendo.2021.759843

    PMID: 34777254

    		 Sample: serum
  10. MOLECULAR NUTRITION & FOOD RESEARCH (2022) IF: 5.82
    Glycine Acts Through Estrogen Receptor Alpha to Mediate Estrogen Receptor Signaling, Stimulating Osteogenesis and Attenuating Adipogenesis in Ovariectomized Rats

    DOI: 10.1002/mnfr.202100857
    Sample: bone mesenchymal stem cell
  11. Materials Science & Engineering C-Materials for Biological Applications (2020) IF: 5.88
    Decellularization and oxidation process of bamboo stem enhance biodegradation and osteogenic differentiation

    DOI: 10.1016/j.msec.2020.111500

    PMID: 33321600

    		 Sample: Mesenchymal stem cell
  12. Scientific Reports (2019) IF: 4.011
    The Role and Mechanism of SIRT1 in Resveratrol-regulated Osteoblast Autophagy in Osteoporosis Rats

    DOI: 10.1038/s41598-019-44766-3

    PMID: 31804494

    		 Sample: Serum
  13. Polymers (2022) IF: 4.329
    Collagen Membrane Derived from Fish Scales for Application in Bone Tissue Engineering

    DOI: 10.3390/polym14132532

    PMID: 35808577

    		 Sample: BMSCs
  14. Antioxidants (2019) IF: 4.52
    Protective Effects of Melon Extracts on Bone Strength, Mineralization, and Metabolism in Rats with Ovariectomy-Induced Osteoporosis

    DOI: 10.3390/antiox8080306

    PMID: 31416178

    		 Sample: Plasma
  15. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020) IF: 4.556
    The Administration of 4-Hexylresorcinol Accelerates Orthodontic Tooth Movement and Increases the Expression Level of Bone Turnover Markers in Ovariectomized Rats

    DOI: 10.3390/ijms21041526
    Sample: blood
  16. Frontiers in Physiology (2023) IF: 4.755
    Relative comparison of chronic kidney disease-mineral and bone disorder rat models

    DOI: 10.3389/fphys.2023.1083725
    Sample: Serum
  17. Polymers (2022) IF: 4.967
    Correlation between Antioxidant and Anti-Osteoporotic Activities of Shilajit Loaded into Chitosan Nanoparticles and Their Effects on Osteoporosis in Rats

    DOI: 10.3390/polym14193972

    PMID: 36235920

    		 Sample: serum
  18. PLoS One (2015) IF: 3.234
    Osteoprotective Effect of Echinocystic Acid, a Triterpone Component from Eclipta prostrata, in Ovariectomy-Induced Osteoporotic Rats

    DOI: 10.1371/journal.pone.0136572
    Sample: Serum
  19. MOLECULES (2020) IF: 3.267
    Protective Effects of Methoxsalen Supplementation on Chronic Alcohol-induced Osteopenia and Steatosis in Rats

    DOI: 10.3390/molecules25051177
    Sample: serum
  20. JOURNAL OF ETHNOPHARMACOLOGY (2020) IF: 3.69
    QingYan formula extracts protect against postmenopausal osteoporosis in ovariectomized rat model via active ER-dependent MEK/ERK and PI3K/Akt signal pathways

    DOI: 10.1016/j.jep.2020.113644

    PMID: 33264660

    		 Sample: serum
  21. CLINICAL ORAL IMPLANTS RESEARCH (2020) IF: 3.723
    Tibolone, alendronate, and simvastatin enhance implant osseointegration in a preclinical in vivo model

    DOI: 10.1111/clr.13602
    Sample: blood
  22. International Journal of Medical Sciences (2021) IF: 3.738
    Self-emulsified annatto tocotrienol improves bone histomorphometric parameters in a rat model of oestrogen deficiency through suppression of skeletal sclerostin level and RANKL/OPG ratio

    DOI: 10.7150/ijms.64045
    Sample: serum
  23. Frontiers in Pharmacology (2018) IF: 3.831
    Metabolomics Based Profiling of Dexamethasone Side Effects in Rats

    DOI: 10.3389/fphar.2018.00046
    Sample: serum
  24. ENDOCRINE (2022) IF: 3.925
    Roxadustat improves renal osteodystrophy by dual regulation of bone remodeling

    DOI: 10.1007/s12020-022-03199-1

    PMID: 36184719

    		 Sample: serum
  25. Annals of Translational Medicine (2021) IF: 3.932
    Conjugated linoleic acid prompts bone formation in ovariectomized osteoporotic rats and weakens osteoclast formation after treatment with ultraviolet B

    DOI: 10.21037/atm-21-934

    PMID: 33850900

    		 Sample: Serum
  26. Acupuncture in Medicine (2018) IF: 2.275
    Long-Term Electroacupuncture Stimulation Prevents Osteoporosis in Ovariectomised Osteopaenic Rats through Multiple Signalling Pathways:

    DOI: 10.1136/acupmed-2016-011268
    Sample: serum
  27. Journal of Orthopaedic Surgery and Research (2022) IF: 2.359
    Roxadustat promotes osteoblast differentiation and prevents estrogen deficiency-induced bone loss by stabilizing HIF-1α and activating the Wnt/β-catenin signaling pathway

    DOI: 10.1186/s13018-022-03162-w

    PMID: 35597989

    		 Sample: serum
  28. CURRENT PHARMACEUTICAL DESIGN (2019) IF: 2.412
    Hyaluronic Acid Modified Risedronate and Teriparatide Co-loaded Nanocarriers for Improved Osteogenic Differentiation of Osteoblasts for the Treatment of Osteoporosis

    DOI: 10.2174/1381612825666190801140703
    Sample: MC3T3-E1 cells
  29. DARU-Journal of Pharmaceutical Sciences (2020) IF: 2.739
    Comparison of diclofenac with apigenin-glycosides rich Clinacanthus nutans extract for amending inflammation and catabolic protease regulations in osteoporotic-osteoarthritis rat model

    DOI: 10.1007/s40199-020-00343-y

    PMID: 32388789

    		 Sample: Serum
  30. ACS Omega (2020) IF: 2.87
    Bioactive Effects of Low-Temperature Argon–Oxygen Plasma on a Titanium Implant Surface

    DOI: 10.1021/acsomega.9b03504
    Sample: osteoblast cell
  31. Molecular Medicine Reports (2015) IF: 2.952
    Radix Dipsaci total saponins stimulate MC3T3-E1 cell differentiation via the bone morphogenetic protein-2/MAPK/Smad-dependent Runx2 pathway

    DOI: 10.3892/mmr.2015.3249
    Sample: Cell lysate
  32. Experimental and Therapeutic Medicine (2017) IF: 1.261
    Protective effects of resveratrol on osteoporosis via activation of the SIRT1‑NF‑κB signaling pathway in rats

    DOI: 10.3892/etm.2017.5147
    Sample: serum
  33. Experimental and Therapeutic Medicine (2015) IF: 1.269
    Electroacupuncture at the governor vessel and bladder meridian acupoints improves postmenopausal osteoporosis through osteoprotegerin/RANKL/RANK and Wnt/β‑catenin signaling pathways

    DOI: 10.3892/etm.2015.2553
    Sample: Serum
  34. BIOELECTROMAGNETICS (2016) IF: 1.583
    Pulsed electromagnetic field at different stages of knee osteoarthritis in rats induced by low‐dose monosodium iodoacetate: Effect on subchondral trabecular bone microarchitecture and cartilage degradation

    DOI: 10.1002/bem.22028
    Sample: Serum,Urine
  35. JOURNAL OF INVESTIGATIVE SURGERY (2022) IF: 1.972
    Acteoside Derived from Cistanche Improves Glucocorticoid-Induced Osteoporosis by Activating PI3K/AKT/mTOR Pathway

    DOI: 10.1080/08941939.2022.2154578
    Sample: serum
  36. Current Developments in Nutrition (2020)
    Symbiotic Effects of Kefir and Yacon Flour (Smallanthus sonchifolius) on Bone Remodeling in Rats

    DOI: 10.1093/cdn/nzaa045_017
    Sample: Urine,Feces
  37. TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH (2018) IF: 0.444
    Bone turnover and oxidative stress markers in estrogendeficient rats treated with Marantodes pumilum leaves and roots: A comparative study

    DOI: 10.4314/tjpr.v17i4.7
    Sample: Blood,Tissue homogenate
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Reviews/Q&A

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  • Reviews
  • Q&A
Q

K************aSubmitted [ Mar 18 2024 ]

Asked: Is this osteocalcin carboxylated or uncarboxylated?

Reply

A

adminSubmitted [ Mar 18 2024 ]

Answered: Hi Katlego, E-EL-R0243 Rat OC/BGP (Osteocalcin) (ELISA) designed for quantification of mouse total, carboxylated and uncarboxylated osteocalcin. As most sample types of mice such as serum and cellular supernatant Osteocalcin are carboxylated status, so this form is mainly detected, and uncarboxylated samples can also be detected if they are present.

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