Current Position:Home>>Kits>>ELISA Kits>> Rat OC/BGP(Osteocalcin) ELISA Kit

Rat OC/BGP(Osteocalcin) ELISA Kit

Citations(37)

Cat.No.:E-EL-R0243
Reactivity: Rat

Filter:

ELISA

To Purchase E-EL-R0243

Size:

96T
48T
24T
96T*5
96T*10

Price: $495

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.78-50 ng/mL
Sensitivity	0.47 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat OC/BGP in samples. No significant cross-reactivity or interference between Rat OC/BGP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat OC/BGP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat OC/BGP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat OC/BGP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat OC/BGP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat OC/BGP. You can calculate the concentration of Rat OC/BGP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 12 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 12 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 12 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat OC/BGP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat OC/BGP were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	2.70	4.70	20.10	2.80	5.10	19.80
Standard deviation	0.10	0.30	1.00	0.20	0.20	0.80
CV (%)	3.70	6.38	4.98	7.14	3.92	4.04

Recovery

The recovery of Rat OC/BGP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	85-98	91
EDTA plasma (n=8)	86-96	91
Cell culture media (n=8)	95-109	102

Linearity

Samples were spiked with high concentrations of Rat OC/BGP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	BGLAP, OT, Bone Gamma-carboxyglutamate Protein, Bone Gla Protein
Research Area	Cancer, Signal transduction, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Frontiers in Physiology (2023) IF: 4.755

Relative comparison of chronic kidney disease-mineral and bone disorder rat models

DOI: 10.3389/fphys.2023.1083725
	Sample: Serum

Frontiers in Endocrinology (2022) IF: 6.055

Investigating the Effects of Diet-Induced Pre-Diabetes on the Functioning of Calcium-Regulating Organs in Male Sprague Dawley Rats: Effects on Selected Markers

DOI: 10.3389/fendo.2022.914189
PMID: 35898447	Sample: Plasma

MOLECULAR NUTRITION & FOOD RESEARCH (2022) IF: 5.82

Glycine Acts Through Estrogen Receptor Alpha to Mediate Estrogen Receptor Signaling, Stimulating Osteogenesis and Attenuating Adipogenesis in Ovariectomized Rats

DOI: 10.1002/mnfr.202100857
	Sample: Bone Mesenchymal Stem Cell

Current Developments in Nutrition (2020)

Symbiotic Effects of Kefir and Yacon Flour (Smallanthus sonchifolius) on Bone Remodeling in Rats

DOI: 10.1093/cdn/nzaa045_017
	Sample: Urine,Feces

TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH (2018) IF: 0.444

Bone turnover and oxidative stress markers in estrogendeficient rats treated with Marantodes pumilum leaves and roots: A comparative study

DOI: 10.4314/tjpr.v17i4.7
	Sample: Blood,Tissue Homogenate

Experimental and Therapeutic Medicine (2017) IF: 1.261

Protective effects of resveratrol on osteoporosis via activation of the SIRT1‑NF‑κB signaling pathway in rats

DOI: 10.3892/etm.2017.5147
	Sample: Serum

Experimental and Therapeutic Medicine (2015) IF: 1.269

Electroacupuncture at the governor vessel and bladder meridian acupoints improves postmenopausal osteoporosis through osteoprotegerin/RANKL/RANK and Wnt/β‑catenin signaling pathways

DOI: 10.3892/etm.2015.2553
	Sample: Serum

BIOELECTROMAGNETICS (2016) IF: 1.583

Pulsed electromagnetic field at different stages of knee osteoarthritis in rats induced by low‐dose monosodium iodoacetate: Effect on subchondral trabecular bone microarchitecture and cartilage degradation

DOI: 10.1002/bem.22028
	Sample: Serum,Urine

JOURNAL OF INVESTIGATIVE SURGERY (2022) IF: 1.972

Acteoside Derived from Cistanche Improves Glucocorticoid-Induced Osteoporosis by Activating PI3K/AKT/mTOR Pathway

DOI: 10.1080/08941939.2022.2154578
	Sample: Serum

Acupuncture in Medicine (2018) IF: 2.275

Long-Term Electroacupuncture Stimulation Prevents Osteoporosis in Ovariectomised Osteopaenic Rats through Multiple Signalling Pathways:

DOI: 10.1136/acupmed-2016-011268
	Sample: Serum

Journal of Orthopaedic Surgery and Research (2022) IF: 2.359

Roxadustat promotes osteoblast differentiation and prevents estrogen deficiency-induced bone loss by stabilizing HIF-1α and activating the Wnt/β-catenin signaling pathway

DOI: 10.1186/s13018-022-03162-w
PMID: 35597989	Sample: Serum

CURRENT PHARMACEUTICAL DESIGN (2019) IF: 2.412

Hyaluronic Acid Modified Risedronate and Teriparatide Co-loaded Nanocarriers for Improved Osteogenic Differentiation of Osteoblasts for the Treatment of Osteoporosis

DOI: 10.2174/1381612825666190801140703
	Sample: Mc3T3-E1 Cells

DARU-Journal of Pharmaceutical Sciences (2020) IF: 2.739

Comparison of diclofenac with apigenin-glycosides rich Clinacanthus nutans extract for amending inflammation and catabolic protease regulations in osteoporotic-osteoarthritis rat model

DOI: 10.1007/s40199-020-00343-y
PMID: 32388789	Sample: Serum

ACS Omega (2020) IF: 2.87

Bioactive Effects of Low-Temperature Argon–Oxygen Plasma on a Titanium Implant Surface

DOI: 10.1021/acsomega.9b03504
	Sample: Osteoblast Cell

Molecular Medicine Reports (2015) IF: 2.952

Radix Dipsaci total saponins stimulate MC3T3-E1 cell differentiation via the bone morphogenetic protein-2/MAPK/Smad-dependent Runx2 pathway

DOI: 10.3892/mmr.2015.3249
	Sample: Cell Lysate

PLoS One (2015) IF: 3.234

Osteoprotective Effect of Echinocystic Acid, a Triterpone Component from Eclipta prostrata, in Ovariectomy-Induced Osteoporotic Rats

DOI: 10.1371/journal.pone.0136572
	Sample: Serum

MOLECULES (2020) IF: 3.267

Protective Effects of Methoxsalen Supplementation on Chronic Alcohol-induced Osteopenia and Steatosis in Rats

DOI: 10.3390/molecules25051177
	Sample: Serum

JOURNAL OF ETHNOPHARMACOLOGY (2020) IF: 3.69

QingYan formula extracts protect against postmenopausal osteoporosis in ovariectomized rat model via active ER-dependent MEK/ERK and PI3K/Akt signal pathways

DOI: 10.1016/j.jep.2020.113644
PMID: 33264660	Sample: Serum

CLINICAL ORAL IMPLANTS RESEARCH (2020) IF: 3.723

Tibolone, alendronate, and simvastatin enhance implant osseointegration in a preclinical in vivo model

DOI: 10.1111/clr.13602
	Sample: Blood

International Journal of Medical Sciences (2021) IF: 3.738

Self-emulsified annatto tocotrienol improves bone histomorphometric parameters in a rat model of oestrogen deficiency through suppression of skeletal sclerostin level and RANKL/OPG ratio

DOI: 10.7150/ijms.64045
	Sample: Serum

Frontiers in Pharmacology (2018) IF: 3.831

Metabolomics Based Profiling of Dexamethasone Side Effects in Rats

DOI: 10.3389/fphar.2018.00046
	Sample: Serum

ENDOCRINE (2022) IF: 3.925

Roxadustat improves renal osteodystrophy by dual regulation of bone remodeling

DOI: 10.1007/s12020-022-03199-1
PMID: 36184719	Sample: Serum

Annals of Translational Medicine (2021) IF: 3.932

Conjugated linoleic acid prompts bone formation in ovariectomized osteoporotic rats and weakens osteoclast formation after treatment with ultraviolet B

DOI: 10.21037/atm-21-934
PMID: 33850900	Sample: Serum

Scientific Reports (2019) IF: 4.011

The Role and Mechanism of SIRT1 in Resveratrol-regulated Osteoblast Autophagy in Osteoporosis Rats

DOI: 10.1038/s41598-019-44766-3
PMID: 31804494	Sample: Serum

Polymers (2022) IF: 4.329

Collagen Membrane Derived from Fish Scales for Application in Bone Tissue Engineering

DOI: 10.3390/polym14132532
PMID: 35808577	Sample: Bmscs

Antioxidants (2019) IF: 4.52

Protective Effects of Melon Extracts on Bone Strength, Mineralization, and Metabolism in Rats with Ovariectomy-Induced Osteoporosis

DOI: 10.3390/antiox8080306
PMID: 31416178	Sample: Plasma

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020) IF: 4.556

The Administration of 4-Hexylresorcinol Accelerates Orthodontic Tooth Movement and Increases the Expression Level of Bone Turnover Markers in Ovariectomized Rats

DOI: 10.3390/ijms21041526
	Sample: Blood

Polymers (2022) IF: 4.967

Correlation between Antioxidant and Anti-Osteoporotic Activities of Shilajit Loaded into Chitosan Nanoparticles and Their Effects on Osteoporosis in Rats

DOI: 10.3390/polym14193972
PMID: 36235920	Sample: Serum

Frontiers in Molecular Biosciences (2021) IF: 5.246

miR-330–5p in Small Extracellular Vesicles Derived From Plastrum testudinis-Preconditioned Bone Mesenchymal Stem Cells Attenuates Osteogenesis by Modulating Wnt/β-Catenin Signaling

DOI: 10.3389/fmolb.2021.679345
	Sample: Serum

Nanomedicine (2022) IF: 5.307

Selenium nanoparticles stimulate osteoblast differentiation via BMP-2/MAPKs/β-catenin pathway in diabetic osteoporosis

DOI: 10.2217/nnm-2021-0401
PMID: 35465693	Sample: Serum

TALANTA (2020) IF: 5.339

Aptamer selection and aptasensor construction for bone density biomarkers

DOI: 10.1016/j.talanta.2020.121818
PMID: 33379043	Sample: Serum

Frontiers in Endocrinology (2021) IF: 5.555

Chondroitin Sulfate Alleviates Diabetic Osteoporosis and Repairs Bone Microstructure via Anti-Oxidation, Anti-Inflammation, and Regulating Bone Metabolism

DOI: 10.3389/fendo.2021.759843
PMID: 34777254	Sample: Serum

Materials Science & Engineering C-Materials for Biological Applications (2020) IF: 5.88

Decellularization and oxidation process of bamboo stem enhance biodegradation and osteogenic differentiation

DOI: 10.1016/j.msec.2020.111500
PMID: 33321600	Sample: Mesenchymal Stem Cell

Frontiers in Cell and Developmental Biology (2021) IF: 6.684

miR-27a-5p—Abundant Small Extracellular Vesicles Derived From Epimedium-Preconditioned Bone Mesenchymal Stem Cells Stimulate Osteogenesis by Targeting Atg4B-Mediated Autophagy

DOI: 10.3389/fcell.2021.642646
	Sample: Serum

CELL BIOLOGY AND TOXICOLOGY (2022) IF: 6.691

Urine-derived stem cells-extracellular vesicles ameliorate diabetic osteoporosis through HDAC4/HIF-1α/VEGFA axis by delivering microRNA-26a-5p

DOI: 10.1007/s10565-022-09713-5
PMID: 35554780	Sample: Serum

JOURNAL OF NANOBIOTECHNOLOGY (2021) IF: 10.435

Remote-controllable bone-targeted delivery of estradiol for the treatment of ovariectomy-induced osteoporosis in rats

DOI: 10.1186/s12951-021-00976-4
PMID: 34407835	Sample: Serum

BIOMATERIALS (2022) IF: 15.304

Immunology and bioinformatics analysis of injectable organic/inorganic microfluidic microspheres for promoting bone repair

DOI: 10.1016/j.biomaterials.2022.121685
PMID: 35953327	Sample: Bmscs

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

K************aSubmitted [ Mar 18 2024 ]

Asked: Is this osteocalcin carboxylated or uncarboxylated?

adminSubmitted [ Mar 18 2024 ]

Answered: Hi Katlego, E-EL-R0243 Rat OC/BGP (Osteocalcin) (ELISA) designed for quantification of mouse total, carboxylated and uncarboxylated osteocalcin. As most sample types of mice such as serum and cellular supernatant Osteocalcin are carboxylated status, so this form is mainly detected, and uncarboxylated samples can also be detected if they are present.

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha