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Rat OC/BGP(Osteocalcin) ELISA Kit

Cat.No.:E-EL-R0243
Reactivity: Rat

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.78-50 ng/mL
Sensitivity	0.47 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat OC/BGP in samples. No significant cross-reactivity or interference between Rat OC/BGP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat OC/BGP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat OC/BGP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat OC/BGP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat OC/BGP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat OC/BGP. You can calculate the concentration of Rat OC/BGP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat OC/BGP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat OC/BGP were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	2.70	4.70	20.10	2.80	5.10	19.80
Standard deviation	0.10	0.30	1.00	0.20	0.20	0.80
CV (%)	3.70	6.38	4.98	7.14	3.92	4.04

Recovery

The recovery of Rat OC/BGP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	85-98	91
EDTA plasma (n=8)	86-96	91
Cell culture media (n=8)	95-109	102

Linearity

Samples were spiked with high concentrations of Rat OC/BGP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	BGLAP, OT, Bone Gamma-carboxyglutamate Protein, Bone Gla Protein
Research Area	Cancer, Signal transduction, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

Antioxidants (2019) IF: 5.014

Protective Effects of Melon Extracts on Bone Strength, Mineralization, and Metabolism in Rats with Ovariectomy-Induced Osteoporosis

DOI: 10.3390/antiox8080306
PMID: 31416178	Sample: Plasma

Scientific reports (2019) IF: 3.998

The Role and Mechanism of SIRT1 in Resveratrol-regulated Osteoblast Autophagy in Osteoporosis Rats

DOI: 10.1038/s41598-019-44766-3
PMID: 31804494	Sample: Serum

Plos One (2015) IF: 2.806

Osteoprotective Effect Of Echinocystic Acid, A Triterpone Component From Eclipta Prostrata, In Ovariectomy-Induced Osteoporotic Rats.

DOI: 10.1371/journal.pone.0136572
PMID: 26317835	Sample: Serum

General physiology and biophysics (2019) IF: 1.07

The potential developmental programming effect of oral curcumin on the bone health and plasma total osteocalcin of male and female rats fed a high-fructose diet during suckling and post weaning

DOI: 10.4149/gpb_2019025
PMID: 31411571	Sample: Plasma

Molecular Medicine Reports (2015) IF: 1.692

Radix Dipsaci Total Saponins Stimulate MC3T3-E1 Cell Differentiation Via The Bone Morphogenetic Protein-2/MAPK/Smad-Dependent Runx2 Pathway.

DOI: 10.3892/mmr.2015.3249
PMID: 25625570	Sample: Cell lysate

Bioelectromagnetics (2017) IF: 1.933

Pulsed Electromagnetic Field At Different Stages Of Knee Osteoarthritis In Rats Induced By Low-Dose Monosodium Iodoacetate: Effect On Subchondral Trabecular Bone Microarchitecture And Cartilage Degradation.

DOI: 10.1002/bem.22028
PMID: 28026095	Sample: Serum,Urine

Experimental & Therapeutic Medicine (2015) IF: 1.261

Electroacupuncture At The Governor Vessel And Bladder Meridian Acupoints Improves Postmenopausal Osteoporosis Through Osteoprotegerin/RANKL/RANK And Wnt/β-Catenin Signaling Pathways.

DOI: 10.3892/etm.2015.2553
PMID: 26622351	Sample: Serum

Daru: journal of Faculty of Pharmacy, Tehran University of Medical Sciences (2020)

Comparison of diclofenac with apigenin-glycosides rich Clinacanthus nutans extract for amending inflammation and catabolic protease regulations in osteoporotic-osteoarthritis rat model

DOI: 10.1007/s40199-020-00343-y
PMID: 32388789	Sample: Serum

Folia Medica Indonesiana (2017)

THE ELEVATION OF OSTEOBLAST ACTIVITY IN RAT BONE MARROW MESENCHYMAL STEM CELLS IN OSTEOGENIC MEDIUM EXPOSED WITH MELATONIN IN PHYSIOLOGICAL DOSES

DOI: 10.20473/fmi.v53i1.5489
	Sample: Cell lysate

Current Developments in Nutrition (2020)

Symbiotic Effects of Kefir and Yacon Flour (Smallanthus sonchifolius) on Bone Remodeling in Rats

DOI: 10.1093/cdn/nzaa045_017
	Sample: Urine,Feces

Clinical Nutrition Experimental (2018)

Effects of Labisia pumila on bone turnover markers and OPG/RANKL system in a rat model of post-menopausal osteoporosis

DOI: 10.1016/j.yclnex.2018.01.002
	Sample: Serum,Urine

BMC Complementary Medicine and Therapies (2020)

Effect of herbal extract Eurycoma longifolia (Physta) on female reproductive hormones and bone biochemical markers: an ovariectomised rat model study

DOI: 10.1186/s12906-020-2814-z
PMID: 32024514	Sample: Serum

The Pan African Medical Journal (2019)

Effects of nicotine on markers of bone turnover in ovariectomized rats

DOI: 10.11604/pamj.2019.33.37.17606
PMID: 31384352	Sample: Serum

Tropical Journal of Pharmaceutical Research (2018) IF: 0.444

Bone turnover and oxidative stress markers in estrogendeficient rats treated with Marantodes pumilum leaves and roots: A comparative study.

DOI: 10.4314/tjpr.v17i4.7
	Sample: Blood,Tissue homogenate

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