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Rat OxLDL(Oxidized Low Density Lipoprotein) ELISA Kit

Cat.No.:E-EL-R0710
Reactivity: Rat

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96T
48T
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96T*5
96T*10

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.47-30 ng/mL
Sensitivity	0.28 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat OxLDL in samples. No significant cross-reactivity or interference between Rat OxLDL and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat OxLDL concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat OxLDL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat OxLDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat OxLDL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat OxLDL. You can calculate the concentration of Rat OxLDL in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat OxLDL were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat OxLDL were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	1.60	2.60	12.20	1.50	2.50	13.00
Standard deviation	0.10	0.10	0.50	0.10	0.10	0.40
CV (%)	6.25	3.85	4.10	6.67	4.00	3.08

Recovery

The recovery of Rat OxLDL spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	95-107	102
EDTA plasma (n=8)	89-104	96
Cell culture media (n=8)	91-103	97

Linearity

Samples were spiked with high concentrations of Rat OxLDL and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	OL
Research Area	Cancer, Cardiovascular, Metabolism, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

ANTIOXIDANTS & REDOX SIGNALING (2022) IF: 7.468
PRDX6 promotes fatty acid oxidation via PLA2-dependent PPARα activation in rats fed high-fat diet

DOI: 10.1089/ars.2022.0065

Sample: serum
Nutrients (2021) IF: 5.719
The TOTUM-63 Supplement and High-Intensity Interval Training Combination Limits Weight Gain, Improves Glycemic Control, and Influences the Composition of Gut Mucosa-Associated Bacteria in Rats on a High Fat Diet

DOI: 10.3390/nu13051569

PMID: 34066988
Sample: Plasma
Nutrients (2021) IF: 5.719
Beneficial Effects of High Intensity Interval Training and/or Linseed Oil Supplementation to Limit Obesity-Induced Oxidative Stress in High Fat Diet-Fed Rats

DOI: 10.3390/nu13103531

PMID: 34684532
Sample: plasma
Pharmaceuticals (2021) IF: 5.863
A Novel Combination Therapy Using Rosuvastatin and Lactobacillus Combats Dextran Sodium Sulfate-Induced Colitis in High-Fat Diet-Fed Rats by Targeting the TXNIP/NLRP3 Interaction and Influencing Gut Microbiome Composition

DOI: 10.3390/ph14040341

Sample: serum
Oxidative Medicine and Cellular Longevity (2016) IF: 4.492
Açai (Euterpe oleracea Mart.) Upregulates Paraoxonase 1 Gene Expression and Activity with Concomitant Reduction of Hepatic Steatosis in High-Fat Diet-Fed Rats

DOI: 10.1155/2016/8379105

Sample: Serum
Oxidative Medicine and Cellular Longevity (2019) IF: 4.868
Tissue-Specific Oxidative Stress Modulation by Exercise: A Comparison between MICT and HIIT in an Obese Rat Model

DOI: 10.1155/2019/1965364

PMID: 31396298
Sample: Plasma,Tissue homogenate
Drug Design Development and Therapy (2015) IF: 3.028
Edible bird’s nest attenuates procoagulation effects of high-fat diet in rats

DOI: 10.2147/DDDT.S87772

Sample: Serum
PHYTOTHERAPY RESEARCH (2019) IF: 3.766
Synergistic effects of the capsaicinoid nonivamide and rosuvastatin on obesity‐related endothelial dysfunction in rat fed a high‐fat diet

DOI: 10.1002/ptr.6369

Sample: blood
Food & Nutrition Research (2015) IF: 2.162
N-Acetylneuraminic acid attenuates hypercoagulation on high fat diet-induced hyperlipidemic rats

DOI: 10.3402/fnr.v59.29046

Sample: Serum
Evidence-based Complementary and Alternative Medicine (2022) IF: 2.63
Edible Bird’s Nest Regulates Hepatic Cholesterol Metabolism through Transcriptional Regulation of Cholesterol Related Genes

DOI: 10.1155/2022/8882993

Sample: serum
Anatomy & Cell Biology (2019)
The effect of alpha-lipoic acid on expression of VCAM-1 in type 2 diabetic rat

DOI: 10.5115/acb.2019.52.2.176

Sample: serum

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