Rat REN(Renin) ELISA Kit

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    • sandwich-Ab-ELISA-Elabscience
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    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience

      Catalog number:E-EL-R0030

      Synonyms:REN1, HNFJ2, renin-1

      Size:
      • 96T
      • 24T
      Qty:
      - +
      Price: $495

      Reactivity: Rat

      Detection Range: 15.63~1000 pg/mL

      Sensitivity: 9.38 pg/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Intended use

      This ELISA kit applies to the in vitro quantitative determination of Rat REN concentrations in Serum, plasma and other biological fluids.

      Test principle

      This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat REN . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat REN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat REN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat REN. You can calculate the concentration of Rat REN in the samples by comparing the OD of the samples to the standard curve.

      Assay type Sandwich
      Format 96T
      Assay time 3.5h
      Reactivity Rat
      Detection Method Colormetric
      Detection Range 15.63—1000 pg/mL
      Sensitivity 9.38 pg/mL
      Sample Volume Required Per Well 100μL
      Sample Type Serum, plasma and other biological fluids

      Specificity

      This kit recognizes Rat REN in samples. No significant cross-reactivity or interference between Rat REN and analogues was observed.

      Typical data

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      Concentration
      (pg/mL)
      O.D Average Corrected
      1000 2.482
      2.52
      2.501 2.425
      500 1.747
      1.757
      1.752 1.676
      250 1.027
      1.003
      1.015 0.939
      125 0.522
      0.56
      0.541 0.465
      62.5 0.27
      0.25
      0.26 0.184
      31.25 0.184
      0.176
      0.18 0.104
      15.63 0.129
      0.129
      0.129 0.053
      0 0.072
      0.08
      0.076 --

      Precision

      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat REN were tested 20 times on one plate, respectively.
      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat REN were tested on 3 different plates, 20 replicates in each plate.

      Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean (pg/mL) 52.60 150.60 411.70 56.30 160.50 427.40
      Standard deviation 3.40 8.90 12.40 3.00 6.90 23.50
      C V (%) 6.46 5.91 3.01 5.33 4.30 5.50

      Recovery

      The recovery of Rat REN spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum (n=5) 88-99 94
      EDTA plasma (n=5) 91-105 96
      Cell culture media (n=5) 93-110 100

      Linearity

      Samples were spiked with high concentrations of Rat REN and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

      Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
      1:2 Range (%) 97-114 93-104 100-114
      Average (%) 104 99 106
      1:4 Range (%) 99-115 81-92 96-112
      Average (%) 106 86 103
      1:8 Range (%) 96-112 85-98 91-104
      Average (%) 102 90 97
      1:16 Range (%) 97-108 87-98 93-109
      Average (%) 102 93 100

      Kit components & Storage

      An unopened kit can be stored at 2-8°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

      Item Specifications Storage
      Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20°C, 6 months
      Reference Standard 2 vials
      Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
      Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20°C(shading light), 6 months
      Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
      Biotinylated Detection Ab Diluent 1 vial, 14 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Substrate Reagent 1 vial, 10 mL 2-8°C(shading light)
      Stop Solution 1 vial, 10 mL 2-8°C
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      • Microplate reader with 450 nm wavelength filter
      • High-precision transfer pipette, EP tubes and disposable pipette tips
      • Incubator capable of maintaining 37°C
      • Deionized or distilled water
      • Absorbent paper
      • Loading slot for Wash Buffer

      Assay Procedure

      elisa assay procedure 1

      1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

      elisa assay procedure 2

      2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

      elisa assay procedure 3

      3. Aspirate and wash the plate for 3 times

      elisa assay procedure 4

      4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

      elisa assay procedure 5

      5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

      elisa assay procedure 6

      6. Add 50μL Stop Solution

      elisa assay procedure 7

      7. Read the plate at 450nm immediately. Calculation of the results

      Citations

      1. Modulation Of The Sympathetic Nervous System By Renal Denervation Prevents Reduction Of Aortic Distensibility In Atherosclerosis Prone ApoE-Deficient Rats.
        IF: 3.786
        Journal: Journal of Translational Medicine(2016)
        DOI: 10.1186/s12967-016-0914-9
        PMID: 27277003
        Products: E-EL-R1430 E-EL-R0030 
        Sample type: Tissue homogenate
        Reactivity: Rat
      2. Two Sulfur Glycoside Compounds Isolated From Lepidium Apetalum Willd Protect NRK52e Cells Against Hypertonic-Induced Adhesion And Inflammation By Suppressing The MAPK Signaling Pathway And RAAS.
        IF: 2.861
        Journal: Molecules(2017)
        DOI: 10.3390/molecules22111956
        PMID: 29137154
        Products: E-EL-R0030 E-EL-R2401 E-EL-R1430 E-EL-R0633 E-EL-0162 E-EL-R0815 E-EL-0070 E-EL-R0792 E-EL-R0893 E-EL-R3012 
        Sample type: Cell culture supernatant
        Reactivity: Rat, Universal
      3. Mechanism of the diuretic activity of Descurainia sophia seed.
        IF: 0.814999999
        Journal: Bangladesh Journal of Pharmacology(2018)
        DOI: 10.3329/bjp.v13i2.35931
        Products: E-EL-R0030 E-EL-R1430 E-EL-R2401 E-EL-0070 E-EL-R0045 E-EL-R0766 
        Sample type: Urine
        Reactivity: Rat
      4. Two Sulfur Glycoside Compounds Isolated from Lepidium apetalum Willd Protect NRK52e Cells against Hypertonic-Induced Adhesion and Inflammation by Suppressing the MAPK Signaling Pathway and RAAS.
        IF: 3.098
        Journal: Molecules(2017)
        DOI: 10.3390/molecules22111956
        PMID: 29137154
        Products: E-EL-R0030 E-EL-R2401 E-EL-R1430 E-EL-0070 E-EL-R0633 E-EL-0162 E-EL-R3012 E-EL-R0792 E-EL-R0893 
        Sample type: Cell culture supernatant
        Reactivity: Rat
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