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Recombinant 2019-nCoV 3C-like Proteinase Protein (His Tag)(Active)

  • Cat.No.:PKSR030465

  • Expression host: E.coli

To Purchase PKSR030465

Size:
  • 10μg
  • 50μg
Price: $220
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Description

Synonyms M Proteinase; 3CL Proteinase
Species Virus
Expression_host E.coli
Sequence Ser1-Gln306
Accession YP_009725301.1
Mol_Mass 36.1 kDa
AP_Mol_Mass 33 kDa
Tag N-6His
Bio_Activity Measured by its ability to cleave a fluorogenic peptide substrate, Dabcyl-KTSAVLQSGFRKME-Edans (Synthetic).The specific activity is >13000pmol/min/mg.

Properties

Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin level < 1.0 EU per µg as determined by the LAL method.
Storage Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
Formulation Supplied as a 0.2 μM filtered solution of PBS, 1mM EDTA, 10% Glycerol, pH 7.4.
Reconstitution Please refer to the printed manual for detailed information.

Background

The viral main proteinase (M pro , also called 3CL pro ), which controls the activities of the coronavirus replication complex. It functions as a cysteine protease engaging in the proteolytic cleavage of the viral precursor polyprotein to a series of functional proteins required for coronavirus replication and is considered as an appealing target for designing anti-SARS agents.

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