English Toll-free:1-888-852-8623 or Contact Us
Keyword cannot be empty !

Recombinant SARS-CoV-2 Papain-like Protease Protein (His Tag)

Cat:PKSR030511
Manual MSDS

Price: $ 1105

Size:
100μg
Quantity:
  • Expression Host: E.coli
  • Accession: YP_009725299.1
  • Applications: Enzyme
Add to cart

For research use only. Order now, ship in 3 days

Product Details
Synonyms Papain-like Protease;PLpro;PL-PRO;pp1a;Replicase polyprotein 1a
Species SARS-CoV-2
Expression Host E.coli
Sequence Glu1564-Val1880
Accession YP_009725299.1
Calculated Molecular Weight 36.8 kDa
Tag N-His
Bio-activity Measured by its ability to cleave a fluorogenic peptide substrate, Arg-Leu-Arg-Gly-Gly-AMC (RLRGGAMC). The Specific Activity is > 80pmols/min/μg
Purity > 90 % as determined by reducing SDS-PAGE.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile 20 mM Tris 500mM NaCl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.
Background The coronaviral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro), are attractive antiviral drug targets because they are essential for coronaviral replication. PLpro has the additional function of stripping ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate immune responses. Targeting PLpro with antiviral drugs may have an advantage in not only inhibiting viral replication but also inhibiting the dysregulation of signaling cascades in infected cells that may lead to cell death in surrounding, uninfected cells.