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Recombinant Cynomolgus Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-Fc)

Cat:PKSQ050112
Manual MSDS

Price: $ 393

Price: $ 132

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50μg 10μg
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  • Expression Host: HEK293 Cells
  • Accession: A0A2K5UY47
  • Applications: BLI
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Product Details
Synonyms Sialic acid-binding Ig-like lectin 15;Siglec-15;CD33 antigen-like 3;CD33L3
Species Cynomolgus macaques
Expression Host HEK293 Cells
Sequence Phe20-Thr263
Accession A0A2K5UY47
Calculated Molecular Weight 53.1 kDa
Observed Molecular Weight 55-70 kDa
Tag C-Fc
Bio-activity Loaded Anti-Human Siglec15 mAb-mFc on AMQ Biosensor, can bind Cynomolgus Siglec-15-Fc with an affinity constant of 0.16 nM as determined in BLI assay. 
Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from a 0.2 μm filtered solution of 50mM Tris-HCl, 100mM Glycine, 150mM NaCl, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.
Background Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO?-terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.