Recombinant Cynomolgus Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-Fc)
Price: $ 393
Price: $ 132
Size:
50μg
10μg
Quantity:
- Expression Host: HEK293 Cells
- Accession: A0A2K5UY47
- Applications: BLI
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Product Details
Synonyms | Sialic acid-binding Ig-like lectin 15;Siglec-15;CD33 antigen-like 3;CD33L3 |
Species | Cynomolgus macaques |
Expression Host | HEK293 Cells |
Sequence | Phe20-Thr263 |
Accession | A0A2K5UY47 |
Calculated Molecular Weight | 53.1 kDa |
Observed Molecular Weight | 55-70 kDa |
Tag | C-Fc |
Bio-activity | Loaded Anti-Human Siglec15 mAb-mFc on AMQ Biosensor, can bind Cynomolgus Siglec-15-Fc with an affinity constant of 0.16 nM as determined in BLI assay. |
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation |
Lyophilized from a 0.2 μm filtered solution of 50mM Tris-HCl, 100mM Glycine, 150mM NaCl, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |
Background | Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO?-terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts. |