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Recombinant Human CD31/PECAM1 Protein (Fc Tag)

Cat:PKSH030454
Manual MSDS

Price: $ 486

Size:
100μg
Quantity:
  • Expression Host: HEK293 Cells
  • Accession: EAW94208.1
  • Applications: Cell culture
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Product Details
Synonyms Platelet endothelial cell adhesion molecule;PECAM-1;EndoCAM;GPIIA;PECA1;CD31;PECAM1
Species Human
Expression Host HEK293 Cells
Sequence Met 1-Lys 601
Accession EAW94208.1
Calculated Molecular Weight 91.2 kDa
Observed Molecular Weight 130-140 kDa
Tag C-hFc
Bio-activity Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8 × 10<sup>4</sup> cells/well are added to sCD31-Fc coated plates (1. 25μg/mL, 100μL/well) in the presence of 20ng/mL PMA, approximately 20-45% will adhere after 30 minutes at 37℃.
Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.
Background The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin &alpha;v&beta;3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.