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Recombinant Human CD32a/FCGR2A Protein (167 His, His Tag)

Cat:PKSH030296
Manual MSDS

Price: $ 732

Price: $ 5875

Size:
100μg 1mg
Quantity:
  • Expression Host: CHO Cells
  • Accession: NP_001129691.1
  • Applications: ELISA
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Product Details
Synonyms Low affinity immunoglobulin gamma Fc region receptor II-a;IgG Fc receptor II-a;CDw32;Fc-gamma RII-a;Fc-gamma-RIIa;FcRII-a;CD32;FCGR2A;FCG2;FCGR2A1;IGFR2;CD32A;CDw32;Fc gamma RIIA;FCG2;FcGR;FCGR2
Species Human
Expression Host CHO Cells
Sequence Met 1-Ile 218
Accession NP_001129691.1
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 29-32 kDa
Tag C-His
Bio-activity Measured by its binding ability in a functional ELISA. Immobilized human CD32a-His (R131H) (CHO) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.09-0.21 μg/ml.
Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.
Background Receptors for the Fc region of IgG (FcγR) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Human FcγRs are divided into three classes designated FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16), which generate multiple isoforms, are recognized. The activating- type receptor either has or associates non-covalently with an accessory subunit that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. FcγRI binds IgG with high affinity and functions during early immune responses, whereas FcγRII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Three genes for human FcγRII (A, B, and C) and one for mouse (FcγRIIB), encoding type I transmembrane proteins with ITAM motifs (FcγRII A and C) or ITIM motifs (FcγRIIB) in their cytoplasmic domains, have been identified. Human CD32, also known as Low affinity immunoglobulin γ Fc region receptor II-a, FcγRII A or FCGR2A Protein, is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Associated with an ITAM-bearing adapter subunit, FcRγ, CD32a delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation, and cytokine production.