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Recombinant Human EphB1/EPHT2 Protein (aa 565-984, His & GST Tag)

Cat:PKSH030344
Manual MSDS

Price: $ 717

Size:
50μg
Quantity:
  • Expression Host: Baculovirus-Insect Cells
  • Accession: AAI11745.1
  • Applications: Enzyme
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For research use only. Order now, ship in 3 days

Product Details
Synonyms Ephrin Type-A Receptor 7;EPH Homology Kinase 3;EHK-3;EPH-Like Kinase 11;EK11;hEK11;EPHA7;EHK3;HEK11
Species Human
Expression Host Baculovirus-Insect Cells
Sequence Arg565-Ala984
Accession AAI11745.1
Calculated Molecular Weight 75.3 kDa
Observed Molecular Weight 66 kDa
Tag N-His-GST
Bio-activity The specific activity was determined to be 140 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.
Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Formulation Supplied as sterile solution of 20mM Tris, 500mM NaCl, 3mM DTT, pH 8.0, 10% glycerol
Reconstitution Not Applicable
Background Ephrin type-B receptor 1, also known as EphB1, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.