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Recombinant Human Interleukin-3/IL-3 Protein (Human Cells, His Tag)

Uniprot : P08700

Cat.No.:PKSH032638
Expression host: HEK293 Cells

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Description

Synonyms	Interleukin-3;IL-3;Hematopoietic Growth Factor;Mast Cell Growth Factor;MCGF;Multipotential Colony-Stimulating Factor;P-Cell-Stimulating Factor;IL3
Species	Human
Expression_host	HEK293 Cells
Sequence	Ala20-Phe152
Accession	P08700
Application	Cell Culture
Mol_Mass	16.1 kDa
AP_Mol_Mass	17-30 kDa
Tag	C-His
Bio_Activity	Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.3-1. 5 ng/ml.

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin level	< 0.01 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background

Interleukin-3 (IL-3) is a potent growth promoting cytokine. IL-3 can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. IL-3 exerts its biological function through binding to specific cell surface receptors. The amino acid sequences of this protein among different species share relatively low identity and its activity is highly species-specific. IL-3 has also been shown to possess neurotrophic activity; and is thought to be associated with neurologic disorders.

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