English Toll-free:1-888-852-8623 or Contact Us
Keyword cannot be empty !

Recombinant Human Periostin/OSF-2 Protein (His Tag)

Cat:PKSH030453
Manual MSDS

Price: $ 732

Size:
100μg
Quantity:
  • Expression Host: HEK293 Cells
  • Accession: NP_001129407.1
  • Applications: Cell culture
Add to cart

For research use only. Order now, ship in 3 days

Product Details
Synonyms Osteoblast-specific factor 2;OSF-2;POSTN;Periostin;PDLPOSTN;periostin;PN
Species Human
Expression Host HEK293 Cells
Sequence Met 1-Gln 781
Accession NP_001129407.1
Calculated Molecular Weight 86.0 kDa
Observed Molecular Weight 80 kDa
Tag C-His
Bio-activity Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. When cells are added to POSTN-His coated plates (10μg/mL, 100μL/well), approximately > 60% will adhere specifically after 30 minutes at 37℃.
Purity > 97 % as determined by reducing SDS-PAGE.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.
Background Periostin ( POSTN ), also known as OSF2 (osteoblast specific factor 2), is a heterofunctional secreted extracellular matrix (ECM) protein comprised of four fasciclin domains that promotes cellular adhesion and movement, as well as collagen fibrillogenesis. Postn is expressed in unique growth centers during embryonic development where it facilitates epithelial-mesenchymal transition (EMT) of select cell populations undergoing reorganization. In the adult, Postn expression is specifically induced in areas of tissue injury or areas with ongoing cellular re-organization. In the adult heart Postn is induced in the ventricles following myocardial infarction, pressure overload stimulation, or generalized cardiomyopathy. Although the detailed function of Postn is still unclear, Postn-integrin interaction is thought to be involved in tumor development. Postn is frequently overexpressed in various types of human cancers, stimulating metastatic growth by promoting cancer cell survival, invasion and angiogenesis, and can be a useful marker to predict the behavior of cancer.