Recombinant Human PLK1 / PLK-1 Protein (His tag)

    • Recombinant Human PLK1 / PLK-1 Protein (His tag)-Elabscience
    • Recombinant protein products for various applications-Elabscience
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    • Recombinant Human PLK1 / PLK-1 Protein (His tag)-Elabscience
    • Recombinant protein products for various applications-Elabscience
    • Recombinant Human PLK1 / PLK-1 Protein (His tag)-Elabscience
    • Recombinant protein products for various applications-Elabscience

      Catalog number:PKSH030399

      Synonyms:PLK;STPK13

      Size:
      • 20µg
      • 50µg
      Qty:
      - +
      Price: $486

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Overview

      Synonyms PLK;STPK13
      Species Human
      Expression_host Baculovirus-Insect Cells
      Sequence Met 1-Ser 603
      Accession NP_005021.2
      Mol_Mass 70.5 kDa
      AP_Mol_Mass 66 kDa
      Tag N-His
      Bio_Activity The specific activity was determined to be 5 nmol/min/mg using casein as substrate.

      Properties

      Purity > 90 % as determined by reducing SDS-PAGE.
      Endotoxin < 1.0 EU per µg as determined by the LAL method.
      Storage Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
      Shipping This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
      Formulation Supplied as sterile 50mM Tris, 100mM NaCl, pH 7.4, 0.5mM EDTA, 0.5mM EGTA, 0.5mM PMSF, 25% glycerol
      Reconstitution Please refer to the printed manual for detailed information.

      Background

      Serine / threonine-protein kinase PLK1 / PLK-1, also known as polo-like kinase 1 (PLK-1) or serine / threonine-protein kinase 13 (STPK13), Polo-like kinases (PLKs), is a family of four serine / threonine protein kinases that are critical regulators of cell cycle progression, mitosis, cytokinesis, and the DNA damage response. PLK1 / PLK-1 is ubiquitously expressed. The mRNA and protein expression of PLK1 / PLK-1, -2 and -4 are coordinately regulated during cell cycle progression, but PLK3 levels are independent of the other three family members. PLK1 / PLK-1 is the most well characterized member of this family and strongly promotes the progression of cells through mitosis. During the various stages of mitosis PLK1 / PLK-1 localizes to the centrosomes, kinetochores and central spindle. PLKs are dysregulated in a variety of human cancers. PLK1 / PLK-1 overexpression correlates with cellular proliferation and poor prognosis. Serine / threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC / C inhibitors, and the regulation of mitotic exit and cytokinesis. It is required for recovery after DNA damage checkpoint and entry into mitosis. PLK1 / PLK-1 is required for kinetochore localization of BUB1B, spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. PLK1 / PLK-1 Phosphorylates BORA, and thereby promotes the degradation of BORA. PLK1 / PLK-1 also contributes to the regulation of AURKA function and phosphorylates SGOL1.

      SDS-PAGE

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