English Toll-free:1-888-852-8623 or Contact Us
Keyword cannot be empty !

Recombinant Human RANKL/TNFSF11 Protein (His Tag)

Cat:PKSH032986
Manual MSDS

Price: $ 739

Price: $ 249

Size:
100μg 20μg
Quantity:
  • Expression Host: E.coli
  • Accession: O14788
  • Applications: Cell culture
Add to cart

For research use only. Order now, ship in 3 days

Product Details
Synonyms CD254;ODF;OPGL;RANKL;TNFSF11;CD254;Osteoclast differentiation factor;Receptor activator of nuclear factor kappa-B ligand;tumor necrosis factor ligand superfamily member 11;hRANKL2;OPTB2;RANKL;sOdf
Species Human
Expression Host E.coli
Sequence Glu 143-Asp317
Accession O14788
Calculated Molecular Weight 20.7 kDa
Observed Molecular Weight 17 kDa
Tag C-His
Bio-activity Measure by its ability to induce osteoclast differentiation in RAW264.7 cells. The ED<sub>50</sub> for this effect is <10 ng/mL.
Purity > 98 % as determined by reducing SDS-PAGE.
Endotoxin < 0.1 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile PBS,pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.
Background CD254, also known as RANKL, TNFSF11, TRANCE, OPGL and ODF, is a type II membrane protein of the tumor necrosis factor (TNF) superfamily, and affects the immune system and control bone regeneration and remodeling. RANKL is the ligand of nuclear factor (NF)-κB (RANK). When RANKL binds to RANK, it will undergo trimerization and then bind to an adaptor molecule TNF receptor-associated factor 6 (TRAF6). This results in the activation of several downstream signaling cascades, including the NFκB, mitogen-activated protein kinases (MAPK), activating protein 1 (AP-1), and nuclear factor of activated T cells (NFATc1), resulting in the formation of multinucleated bone-resorbing osteoclasts. RANKL is widely expressed in skeletal muscle, thymus, liver, colon, small intestine, adrenal gland, osteoblast, mammary gland epithelial cells, prostate and pancreas.