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Recombinant Human SCF/c-Kit Ligand Protein (His Tag)

Uniprot : P21583

Cat.No.:PKSH033081
Expression host: HEK293 Cells

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Description

Synonyms	Kit Ligand;Mast Cell Growth Factor;MGF;Stem Cell Factor;SCF;c-Kit ligand;KITLG;MGF;SCF
Species	Human
Expression_host	HEK293 Cells
Sequence	Glu26-His214
Accession	P21583
Application	Cell Culture
Mol_Mass	22.0 kDa
AP_Mol_Mass	30-50 kDa
Tag	C-His
Bio_Activity	Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 2-10 ng/ml.

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed man
Reconstitution	Please refer to the printed manual for detailed information.

Background

Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid; erythroid; and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors.

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