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Recombinant Human Wnt3a/Wnt-3a

  • Cat.No.:PKSH033972

  • Expression host: HEK293 Cells
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Description

Synonyms MGC119418;MGC119419;MGC119420;protein Wnt-3a;wingless-type MMTV integration site family;member 3A;Wnt3a;Wnt-3a
Species Human
Expression_host HEK293 Cells
Mol_Mass 58.5 kDa
AP_Mol_Mass 66-73 kDa
Tag None
Bio_Activity Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.The ED50 for this effect is 71.45 ng/ml.

Properties

Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin level < 0.01 EU per μg of the protein as determined by the LAL method.
Storage Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Formulation Supplied as a 0.2 μm filtered solution of 25mM Tris-HCl, 500mM NaCl, pH8.2.
Reconstitution Not Applicable

Background

Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes.Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro).

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