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Recombinant Mouse β-NGF/Beta-NGF Protein

Cat:PKSM040709
Manual MSDS

Price: $ 220

Size:
20μg
Quantity:
  • Expression Host: CHO Stable Cells
  • Accession: NP_001106168.1
  • Applications: Cell culture
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Product Details
Synonyms Ngfb
Species Mouse
Expression Host CHO Stable Cells
Sequence Ser 122-Gly 241
Accession NP_001106168.1
Calculated Molecular Weight 13.5 kDa
Observed Molecular Weight 13.5 kDa
Tag None
Bio-activity Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 1-8ng/mL.
Purity > 96 % as determined by reducing SDS-PAGE.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile 20mM NaAc, 150mM NaCl, pH 5.5
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.
Background Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, &alpha;, &beta;, and &gamma;, among which, the &beta; subunit, called &beta;-NGF (beta-NGF), was demonstrated to exhibits the growth stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In addition, previous studies indicated that NGF may also have an important role in the regulation of the immune system.