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Recombinant Mouse IL7RA/CD127 Protein (Fc Tag)

  • Cat.No.:PKSM041242

  • Expression host: HEK293 Cells
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Description

Synonyms Interleukin-7 receptor subunit alpha;Il7r;IL-7 receptor subunit alpha;IL-7R subunit alpha;IL-7R-alpha;IL-7RA;CD127;IL-7Ralpha
Species Mouse
Expression_host HEK293 Cells
Sequence Glu21-Asp239
Accession P16872
Application Functional ELISA
Mol_Mass 52.1 kDa
AP_Mol_Mass 61-75 kDa
Tag C-Fc
Bio_Activity Loaded Human IL-7-His on HIS1K Biosensor, can bind Mouse IL-7RA-Fc with an affinity constant of 5.91 nM as determined in BLI assay.

Properties

Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin level < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 10% Sucrose, 3% Glycine, 100mM NaCl, 0.1mM EDTA, 0.05% Tween 80, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please
Reconstitution Please refer to the printed manual for detailed information.

Background

Interleukin 7 Receptor alpha (IL-7Rα), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival. IL-7Rα is majorly expressed on T cells and their precursors, and early in B cell development as well, prior to the appearance of surface IgM. Dynamic regulation of IL-7Rα is important for the generation of appropriate immune responses.

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