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Recombinant Mouse Interleukin-7/IL-7 Protein (His Tag)

Uniprot : P10168

Cat.No.:PKSM041098
Expression host: HEK293 Cells

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Description

Synonyms	IL-7;IL-7 interleukin-7;interleukin-7;Lymphopoietin -1;PBGF
Species	Mouse
Expression_host	HEK293 Cells
Sequence	Glu26-Ile154
Accession	P10168
Application	Cell Culture
Mol_Mass	15.9 kDa
AP_Mol_Mass	22-28 kDa
Tag	C-His
Bio_Activity	Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL).The ED₅₀ for this effect is 60-1000 pg/ml.

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background

Mouse interleukin-7(IL-7) is the member of hemopoietin family which is important to the differentiation, proliferation, and survival of lymphocyte. Mouse IL-7 shares approximately 88% aa sequence identity with rat IL-7 and 58-60% with human, equine, bovine, ovine, porcine, feline and canine IL-7. It is widely expressed in primary and secondary lymphoid tissues cell and stromal epithelial cells of the thymus, bone marrow, and intestines. IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development. It is important for proliferation during certain stages of B-cell maturation. IL-7 contributes to the maintenance of all na?ve and memory T cells, mainly by promoting expression of the anti-apoptotic protein Bcl-2. It is required for optimal T cell-dendritic cell interaction.

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