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Recombinant Mouse PD-1/PDCD1 Protein (Fc Tag)(Active)

  • Cat.No.:PKSM041289

  • Expression host: Human Cells

To Purchase PKSM041289

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  • 10μg
  • 50μg
Price: $62
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Description

Synonyms Programmed cell death protein 1;PD-1;CD279;Pdcd1;mPD-1
Species Mouse
Expression_host Human Cells
Sequence Leu25-Gln167
Accession Q02242
Application Functional ELISA
Mol_Mass 43.3 kDa
AP_Mol_Mass 58-85 kDa
Tag C-Fc
Bio_Activity Immobilized Human PD-L1-His(Cat: PKSH033557) at 2μg/ml(100 μl/well) can bind Mouse PD-1-Fc.

Properties

Purity > 95% as determined by reducing SDS-PAGE.
Endotoxin level < 1.0 EU per μg as determined by the LAL method.
Storage Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH8.0.
Reconstitution Please refer to the printed manual for detailed information.

Background

Programmed Death-1 (PD-1), firstly cloned from mouse T cell hybridoma 2B4.11, is one member of CD28/CTLA-4 superfamily. PD-1 belongs to type I transmembrane protein and acts as an important immunosuppressive molecule. This family also include members of CD28, CTLA-4 and ICOS.The mouse Programmed Death-1 protein, encoded by PD-1 gene, comprises four parts including a putative 20 aa signal peptide, a 149 aa extracellular region, a 21 aa transmembrane domain and a 98 aa cytoplasmic region. The cytoplamsic tail of PD-1 contains two structural motifs, an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM) formed by two tyrosine residues which make the difference in PD-1 signal mediating. Mouse PD-1 is expressed in thymus and shares about 69% aa sequence identity with human PD-1. Recently, programmed death-1 (PD-1) with its ligands, programmed death ligand B7H1 (PD-L1) and B7DC (PD-L2), was found to regulate T-cell activation and tolerance, upon ligand binding, inhibiting T-cell effector functions in an antigen-specific manner. PD-1 gene knocked out mice would induce some autoimmune diseases, which suggests that PD-1 acts as a co-inhibitory molecule actively participating in maintaining peripheral tolerance. Thus, PD-1 may be a useful target for the immunologic therapy of carcinoma,infection,autoimmune diseases as well as organ transplantation.

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