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Recombinant Mouse TNFRSF1B/CD120b Protein (His Tag)

Cat:PKSM040846
Manual MSDS

Price: $ 732

Size:
100μg
Quantity:
  • Expression Host: HEK293 Cells
  • Accession: NP_035740.2
  • Applications: Cell culture
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Product Details
Synonyms CD120b;p75;TNF-alphaR2;TNF-R-II;TNF-R2;TNF-R75;TNFalpha-R2;TNFBR;Tnfr-1;Tnfr2;TNFR80;TNFRII;Tumor necrosis factor receptor superfamily member 1b;Tnfrsf1b
Species Mouse
Expression Host HEK293 Cells
Sequence Met 1-Gly 258
Accession NP_035740.2
Calculated Molecular Weight 26.8 kDa
Observed Molecular Weight 45 kDa
Tag C-His
Bio-activity Measured by its ability to inhibit TNFα-mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 1-3 μg/mL in the presence of 0.1 ng/mL of recombinant mouse TNFα.
Purity > 97 % as determined by reducing SDS-PAGE.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.
Background Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B), also known as Tumor necrosis factor receptor 2 (TNFR2) or CD120b antigen, is a member of the tumor necrosis factor receptor superfamily. TNFR2/CD120b/TNFRSF1B is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2/CD120b/TNFRSF1B is not a major contributing factor to the genetic risk of type 2 diabetes, its associated peripheral neuropathy and hypertension and related metabolic traits in North Indians. Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B) has been reported to be associated with SLE risk in Japanese populations. TNFR2/CD120b/TNFRSF1B serves as a receptor with high affinity for TNFSF2 and approximately 5-fold lower affinity for homotrimeric TNFSF1. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.