RPMI-1640 is an improved Mccoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media. RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.

General Information

Product Form	Liquid
Concentration	Ready-to-use
Component	RPMI-1640(PM150110)+Nutrients+1% PS(PB180120)+2mM L-Alanyl-L-Glutamine(PB180419)
Bacterial detection	Negative
Fungal detection	Negative
Mycoplasma detection	Negative
Endotoxin content	＜3
Storage	2-8℃ ,Shading Light
Shipping	Ice bag
Expiration date	3 months

Notes

1. This product is for research use only.;
2. This product is sterilized by 0.1μm filtration.;
3. It is necessary to pay attention to the aseptic operation and avoid the contamination during the culture.;
4. It is not suitable for long time storage at room temperature.;
5. This product is a ready-to-use medium. If there is no special need, don’t add serum, penicillin and streptomycin. It can be used directly.

Technical Resources

Recommendations for handling of cryopreserved cells

Protocol for Thawing Frozen Cells

Count the number of cells with Trypan Blue exclusion

Citations

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