RPMI-1640 is an improved Mccoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media. RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.

General Information

Product Form	Powder
pH	7.2-7.4
D-Glucose	2000mg/L
HEPES	negative
L-glutamine	2mM
NaHCO₃	negative
Phenol red	5mg/L
Sodium pyruvate	negative
Storage	2-8℃ ,Shading Light
Shipping	RT
Expiration date	36 months

Notes

1. This product is for research use only.;
2. It is necessary to pay attention to the aseptic operation and avoid the contamination.

Technical Resources

Recommendations for handling of cryopreserved cells

Protocol for Thawing Frozen Cells

Count the number of cells with Trypan Blue exclusion

Citations

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