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S100A9 Polyclonal Antibody

Cat:E-AB-40341
Manual MSDS
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Price: $ 380

Price: $ 230

Price: $ 125

Price: $ 65

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Mouse;Rat
  • Applications: WB;IHC
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For research use only. Order now, ship in 3 days

Product Details
Verified Samples Verified Samples in WB:Mouse lung,Rat spleen,Rat lung
Verified Samples in IHC:Mouse spleen,Mouse spleen
Dilution

WB:1:250-1:500, IHC 1:100-1:300

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Recombinant Mouse S100A9 protein expressed by E.coli
Abbre S100A9
Synonyms Leukocyte L1 complex heavy chain;60B8AG;CAGB;Calgranulin B;Calgranulin-B;Calprotectin L1H subunit;CFAG;CGLB;Cystic fibrosis antigen B;L1AG;Leukocyte L1 complex heavy chain;LIAG;MAC387;MIF;Migration inhibitory factor related protein 14;Migration inhibitory factor-related protein 14;MRP 14;MRP-14;MRP14;Myeloid-related protein 14;NIF;OTTHUMP00000015331;p14;Protein S100-A9;S100 A9;S100 calcium binding protein A9;S100 calcium binding protein A9 calgranulin B;S100 calcium-binding protein A9;S100A9;S10A9
Swissprot
Calculated MW 13 kDa
Observed MW 14 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Secreted
Concentration 0.8mg/mL
Buffer PBS with 0.05% Proclin300 and 50% glycerol, pH7.4.
Purification Method Antigen Affinity Purification
Research Areas Cancer; Immunology; Signal Transduction
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Biological ice pack at 4 ℃
background he protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21.