English Toll-free:1-888-852-8623 or Contact Us
Keyword cannot be empty !

STK4 Polyclonal Antibody

Cat:E-AB-40157
Manual MSDS

Price: $ 380

Price: $ 230

Price: $ 125

Price: $ 65

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human;Rat
  • Applications: WB;IHC
Add to cart

For research use only. Order now, ship in 3 days

Product Details
Verified Samples Verified Samples in WB:293T,Rat liver
Verified Samples in IHC:Rat lung
Dilution

WB 1:500-1:1000, IHC 1:50-1:100

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Recombinant human Serine/threonine-protein kinase 4 protein
Abbre STK4/MST
Synonyms Antigen NY-CO-13;BCC7;Cellular tumor antigen p53;FLJ92943;LFS1;Mutant tumor protein 53;p53;p53 tumor suppressor;P53;Phosphoprotein p53;Tp53;Transformation related protein 53;TRP53;Tumor protein 53;Tumor protein p53;Tumor suppressor p53
Swissprot
Calculated MW 56 kDa
Observed MW 56 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm. Nucleus. The caspase-cleaved form cycles between the nucleus and cytoplasm.
Concentration 2mg/mL
Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Purification Method Affinity purification
Research Areas Cancer; Cell Biology; Epigenetics and Nuclear Signaling
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Biological ice pack at 4 ℃
background The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process.