Succinate Dehydrogenase (SDH) Colorimetric Assay Kit

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K050

      Size:
      • 50 Assays
      Qty:
      - +
      Price: $200

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometry (Visible range)

      Valid period: 3 months

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Application

      This kit can be used for detection of succinate dehydrogenase (SDH) activity in samples, such as tissue, serum (plasma) and other samples. 


      Detection significance

      Succinate Dehydrogenase is mitochondrial-bound enzyme, which belongs to membrane-bound enzyme, is one of the key position that connect oxidative phosphorylation and electron transfer. It can provide electrons for aerobic and capacity respiratory chain of eukaryotic mitochondria and many prokaryotic cells, and is considered as a marker enzyme of mitochondrial. As a key enzyme in the citric acid cycle, SDH is one of the indicator which reflects the mitochondrial function, its activity can be used as evaluation index of three acid cycle operation. It has an important significance for the evaluation of sperm mitochondrial function and the study of ketosis deficiency pathological process in cow, etc.

       

      Detection principle

      FAD participate in the reaction catalyzed by succinic dehydrogenase (SDH) as the prosthetic group, and FAD is reduced to FADH. This reaction is coupled with the reducing of 2,6-DPIP, thus the SDH activity can be calculated by determining the reduction rate of 2,6-DPIP.  


      Experimental instrument

      Test tube, Micropipettor, Vortex mixer, 37 water bath, Spectrophotometer (600 nm)


      Operation steps

      1. Set the spectrophotometer to zero with with double-distilled water at 600 nm with 1 cm diameter cuvette.

      2. Pre-heat the working solution at 37 for more than 5 min.

      3. Operation table

       

      Sample tube

      Sample (μL)

      100

      Working solution (μL)

      2600

      Mix fully and record the time immediately, measure the absorbance at 600 nm with 1 cm diameter cuvette at 5 second (A1) and 65 second (A2), respectively. A=A1-A2.

      [Notes]

      (1) The Working solution must be stored in the dark. The Working solution in the detection procedure should also be protected from light.

      (2) The cuvette must be rinsed out with running water and washed with double distilled water for 2~3 times before next detection.

      (3) After adding sample into the cuvette, the timing should be synchronically with the adding of reagent.

      (4) The working solution must be pre-heated before detection.


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