SUD (SudanⅠ) ELISA Kit

    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
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    >
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience

      Catalog number:E-FS-E016

      Size:
      • 96T
      Qty:
      - +
      Price: $240

      sample_type: Tomato juice,Ketchup,Chilli sauce,Chill ipowder,Feed,Eggss

      Sensitivity: 0.3 ppb

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Test principle

      This kit uses Competitive-ELISA as the method. It can detect Sudan (SUD) in samples, such as tomato juice, chilli sauce, eggs, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, SUD in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-SUD antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of SUD. The concentration of SUD in the samples can be calculated by comparing the OD of the samples to the standard curve.


      Technical indicator

      Sensitivity: 0.3 ppb (ng/mL)

      Reaction mode: 25, 30min~ 30min~15min

      Detection limit: Tomato juice, Ketchup, Chilli sauce ---12ppb; Chilli powder, Feed ---120ppb; Eggs ---30ppb

      Cross-reactivity: Sudan Ⅰ---100%, Para Red ---123%, Rhodamine ---8%,

      Sample recovery rate: Tomato juice, Ketchup, Chilli sauce ---80%±15%,

      Chilli powder, Feed ---95%±15%; Eggs ---80%±15%


      Kits components

      Item

      Specifications

      ELISA Microtiter plate

      96 wells

      High Concentrated Standard (1.0ppm) 

      1 mL

      Standard Liquid   (empty bottles)

      0ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb, 24.3 ppb

      HRP Conjugate

      11 mL

      Antibody Working Solution

      5.5 mL

      Substrate Reagent A

      6 mL

      Substrate Reagent B

      6 mL

      Stop Solution

      6 mL

      20×Concentrated Wash Buffer

      40 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy


      Other materials required but not supplied

      Instruments: Microplate reader, Printer, Homogenizer, Nitrogen evaporators, Water bath, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).

      High-precision transferpettor: single channel (20-200 μL, 100-1000 μL), Multichannel(300 μL).

      Reagent: Methanol.


      Storage and valid period

      Store at 2~8for 1 year. Avoid freeze.

      Please store the opened kit at 2~8, protect from light and moisture. The valid period is 1 months.

      Expiry date:  expiration date is on the packing box.

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