TGF β Receptor I Monoclonal Antibody

  • Pathological antibodies-Elabscience
    • Pathological antibodies-Elabscience
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    • Pathological antibodies-Elabscience
    • Pathological antibodies-Elabscience
      Catalog number:E-PA-3401

      Synonyms:TGF-beta receptor type-1,TGFR-1,Activin A receptor type II-like protein kinase of 53kD,Activin receptor-like kinase 5,ALK-5,ALK5,Serine/threonine-protein kinase receptor R4,SKR4,TGF-beta type I receptor,Transforming growth factor-beta receptor type I,TGF-

      Size:
      • 0.1mL
      • 0.2mL
      Qty:
      - +
      Price: Inquire

      Reactivity: Human

      Applications: IHC-p

      Lead Time: 7~10 daysWelcome to order from local distributors.

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      Background

      Background TGFBR1 (Transforming Growth Factor Beta Receptor 1) is a Protein Coding gene. Diseases associated with TGFBR1 include Multiple Self-Healing Squamous Epithelioma, Susceptiblity To and Loeys-Dietz Syndrome 1. Among its related pathways are PEDF Induced Signaling and SMAD2/3 MH2 Domain Mutants in Cancer. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is ACVR1B.

      Overview

      Synonyms TGF-beta receptor type-1,TGFR-1,Activin A receptor type II-like protein kinase of 53kD,Activin receptor-like kinase 5,ALK-5,ALK5,Serine/threonine-protein kinase receptor R4,SKR4,TGF-beta type I receptor,Transforming growth factor-beta receptor type I,TGF-
      Swissprot P36897
      Source Mouse
      Reactivity Human
      Immunogen Full length protein from eukaryotic expression system.
      Application IHC-p
      Recommended dilution IHC 1:500
      Concentration 1mg/mL
      Clonality Monoclonal

      Properties

      Positive_control Malignant tumour
      Cellular localization Cytoplasm
      Specificity This antibody detects endogenous levels of TGF β Receptor I
      Clone No. Clone:2F2
      Isotype IgG
      Purify Antigen affinity purification
      Conjugation Unconjugated
      Storage instructions Store at -20℃, Avoid freeze / thaw cycles
      Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      Function Transmembrane serine/threonine kinase forming with the TGF-beta type II serine/threonine kinase receptor, TGFBR2, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFBR1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways. For instance, TGFBR1 induces TRAF6 autoubiquitination which in turn results in MAP3K7 ubiquitination and activation to trigger apoptosis. Also regulates epithelial to mesenchymal transition through a SMAD-independent signaling pathway through PARD6A phosphorylation and activation.

      Images

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon caner tissue labeling TGF β Receptor I Monoclonal Antibody with E-PA-3232 at dilution of 1:500. Heat induced epitope retrieval was performed by boiling the tissue sections in citrate buffer(PH6.0)for 20 minutes. Incubation with the primary antibody was performed for overnight at 4℃ followed by keeping at room temperature for 15mins. HRP conjugated Anti-Mouse secondary antibody was applied for 30 minutes at room temperature followed by DAB as chromogen, then counterstain with haematoxylin.
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