TMP (Trimethoprim) ELISA Kit

    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience

      Catalog number:E-FS-E020

      • 96T
      - +
      Price: $340

      sample_type: Feed,Tissue,Kidney,Liver,Serum,Urine

      Sensitivity: 0.03 ppb

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Test principle

      This kit uses Competitive-ELISA as the method. It can detect Trimethoprim (TMP) in samples, such as tissue, feed, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, TMP in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-TMP antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of TMP. The concentration of TMP in the samples can be calculated by comparing the OD of the samples to the standard curve.

      Technical indicator

      Sensitivity: 0.03 ppb (ng/mL)

      Reaction mode: 37, 45 min ~ 15 min

      Detection limit: Feed---1.6 ppb; Tissue, Kidney, Liver ---0.4 ppb; Serum, Urine ---0.4 ppb

      Cross-reactivity: Trimethoprim---100%, Sulfapyridine---0.1%, Sulfamethoxazole---0.1%,

           Sulfisoxazole---0.1%, Sulfathiazole---0.1%, Sulfamerazine ---0.1%,

                                    Sulfadoxine ---0.1%

      Sample recovery rate: Feed---95%±15%; Tissue, Liver, Kidney---95%±10%, Serum, Urine ---85%±10%

      Kits components



       ELISA Microtiter plate

      96 wells

      Standard Liquid 

      1 mL each

      (0 ppb, 0.03 ppb, 0.09 ppb, 0.27 ppb, 0.81 ppb, 2.43 ppb)

      HRP Conjugate

      5.5 mL

      Substrate Reagent A

      6 mL

      Substrate Reagent B 

      6 mL

      Stop Solution

      6 mL

      20×Concentrated Wash Buffer 

      40 mL

      Reconstitution Buffer 

      50 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece


      1 copy

      Other supplies required

      Instruments: Microplate reader, Printer, Homogenizer, Nitrogen Evaporators, Water bath, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g). 

      High-precision transferpettor: single channel (20-200 μL, 100-1000 μL), Multichannel (300 μL).

      Reagents: Absolute methanol, N-hexane, Concentrated Hydrochloric acid (HCl), Sodium hydroxide (NaOH).

      Storage and valid period

      Store at 2~8for 1 year. Avoid freeze.

      Please store the opened kit at 2~8, protect from light and moisture. The valid period is 1 months.

      Expiry date:  expiration date is on the packing box.

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      MSDS for ELISA ISO 9001

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