TMP(Trimethoprim) ELISA Kit

    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
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    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience

      Catalog number:E-FS-E023

      Size:
      • 96T
      Qty:
      - +
      Price: $360

      sample_type: Muscle,Feed,Serum,Urine

      Sensitivity: 0.015 ppb

      reactivity: Universal

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Test principle

      This kit uses Competitive-ELISA as the method. It can detect Trimethoprim (TMP) in samples, such as muscle, feed, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, TMP in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-TMP antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of TMP. The concentration of TMP in the samples can be calculated by comparing the OD of the samples to the standard curve.


      Technical indicator

      Sensitivity: 0.015 ppb

      Reaction mode: 37℃, 45 min~15 min

      Reaction rate: Trimethoprim (TMP) ---100%

      Detection limit:  Feed---0.8 ppb; Muscle---0.2 ppb; Serum, Urine---0.2 ppb

      Cross-reactivity: Trimethoprim ---100%, Sulfapyridine---<0.1%, Sulfadiazine---<0.1%, 

                                    Sulfisoxazole---<0.1%, Sulfathiazole ---<0.1%, Sulfamerazine---<0.1%,

                                   Sulfadoxine---<0.1%,

      Sample recovery rate: Feed---85%±10%; Serum, Urine---85%±10%; Muscle --- 85%±15%


      Kits components

      Item

      Specifications

      ELISA Microtiter plate

      96 wells

      Standard Liquid

      1 mL each

      (0 ppb, 0.015 ppb, 0.045 ppb, 0.135 ppb, 0.405 ppb, 1.215 ppb)

      HRP Conjugate

      5.5 mL

      Substrate Reagent A

      6 mL

      Substrate Reagent B

      6 mL

      Stop Solution

      6 mL

      20×Concentrated Wash Buffer

      40 mL

      2×Reconstitution Buffer

      50 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy


      Other materials required but not supplied

      Instruments: Microplate reade, Printer, Homogenizer, Nitrogen evaporators, Water bath, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).

      Micropipette: Single channel (20-200 μL, 100-1000 μL), Multichannel (30-300 μL).

      Reagents: Anhydrous methanol, N-hexane, NaOH, Concentrated HCl.


      Storage and expiry date

      Store the kit at 2~8. Do not freeze any test kit components.

      Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 - 8.

      Expiry date: expiration date is on the packing box.

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      MSDS for ELISA ISO 9001

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