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Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit

Cat:E-BC-K097-M Citations (24)
Manual MSDS

Price: $ 280

Size:
96T
Quantity:
  • Sample Type: serum;plasma;animal tissue;red blood cells;cultured cells
  • Detection Instrument: Microplate reader(405-415 nm,optimum wavelength: 412 nm)
  • Reasearch Areas: Oxidative stress;cuproptosis;Ferroptosis
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Product Details
Detection Principle GSSG is reduced to GSH by glutathione reductase, and GSH can react with DTNB to produce GSSG and yellow TNB. The amount of total glutathione (GSSG+GSH) determines the amount of yellow TNB. Thus the total glutathione can be calculated by measuring the OD value at 412 nm. The content of GSSG can be determined by first removing GSH from the sample with appropriate reagent and then using the above reaction principle.
Synonyms T-GSH;GSSG
Sample Type serum,plasma,animal tissue,red blood cells,cultured cells
Sensitivity 0.36 μmol/L T-GSH
Detection Range 0.36-30 μmol/L T-GSH
Detection Method Colorimetric method
Assay Time 100 min
Precision inter-assay CV: 3.9% | intra-assay CV: 0.6%
Other Reagents Required PBS (0.01 M, pH 7.4), Absolute ethanol
Storage This product can be stored at -20°C for 12 months with shading light.
Valid Period 12 months
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Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Human plasma 1
10% Rat liver tissue homogenate 10-20
10% Rat kidney tissue homogenate 1
10% Rat heart tissue homogenate 10-20
10% Mouse brain tissue homogenate 2-5
HepG2 cells 1
The diluent is protein precipitator. For the dilution of other sample types, please do pretest to confirm the dilution factor.