Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit
Price: $ 280
- Sample Type: serum;plasma;animal tissue;red blood cells;cultured cells
- Detection Instrument: Microplate reader(405-415 nm,optimum wavelength: 412 nm)
- Reasearch Areas: Oxidative stress;cuproptosis;Ferroptosis
For research use only. Order now, ship in 3 days
Detection Principle | GSSG is reduced to GSH by glutathione reductase, and GSH can react with DTNB to produce GSSG and yellow TNB. The amount of total glutathione (GSSG+GSH) determines the amount of yellow TNB. Thus the total glutathione can be calculated by measuring the OD value at 412 nm. The content of GSSG can be determined by first removing GSH from the sample with appropriate reagent and then using the above reaction principle. | ||||||||||||||
Synonyms | T-GSH;GSSG | ||||||||||||||
Sample Type | serum,plasma,animal tissue,red blood cells,cultured cells | ||||||||||||||
Sensitivity | 0.36 μmol/L T-GSH | ||||||||||||||
Detection Range | 0.36-30 μmol/L T-GSH | ||||||||||||||
Detection Method | Colorimetric method | ||||||||||||||
Assay Time | 100 min | ||||||||||||||
Precision | inter-assay CV: 3.9% | intra-assay CV: 0.6% | ||||||||||||||
Other Reagents Required | PBS (0.01 M, pH 7.4), Absolute ethanol | ||||||||||||||
Storage | This product can be stored at -20°C for 12 months with shading light. | ||||||||||||||
Valid Period | 12 months | ||||||||||||||
Images | |||||||||||||||
Dilution Of Sample |
The recommended dilution factor for different samples is as follows (for reference only):
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The diluent is protein precipitator. For the dilution of other sample types, please do pretest to confirm the dilution factor. |
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