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Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (ABTS, Chemical Method)

Cat.No.:E-BC-K271-M
Detection instrument: Microplate reader(730-740 nm)

Detection method: Colorimetric method

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Detection principle

The principle of the ABTS method for determining the T-AOC is as follows. ABTS is oxidized to green ABTS+ by appropriate oxidant, which can be inhibited if there exist antioxidants. The T-AOC of the sample can be determined and calculated by measuring the absorbance of ABTS+ at 734 nm. Trolox is an analog of VE and has a similar antioxidant capacity to that of VE. Trolox is used as a reference for other antioxidant antioxidants. For example, the T-AOC of Trolox is 1, then the antioxidant capacity of the other substance with the same concentration is showed by the ratio of its antioxidant capacity to Trolox antioxidant capacity.

Performance characteristics

Synonyms	T-AOC
Sample type	Serum,plasma,urine,saliva,tissue,cells
Sensitivity	0.05 mmol/L
Detection range	0.05-1.00 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	20 min
Precision	Average inter-assay CV: 5%Average intra-assay CV: 4.100%
Other instruments required	Micropipettor, Centrifuge, Vortex mixer
Other reagents required	Double distilled water, 1 × PBS, 80% Ethanol
Storage	-20℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.05-1.00 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
20% Tomato tissue homogenization	2-5
10% Mouse heart tissue homogenization	8-12
10% Mouse liver tissue homogenization	8-12
10% Mouse lung tissue homogenization	8-12
Human saliva	2-5
Human urine	15-30
Human serum	15-30
Human plasma	8-15

Note: The diluent is 1 × PBS or 80% ethanol.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Foods (2023) IF: 5.561
Treatment of Diabetes Nephropathy in Mice by Germinating Seeds of Euryale ferox through Improving Oxidative Stress

DOI: 10.3390/foods12040767

Sample: Serum
Frontiers in Pharmacology (2021) IF: 5.811
The Anti-Inflammatory Effect of a Combination of Five Compounds From Five Chinese Herbal Medicines Used in the Treatment of COPD

DOI: 10.3389/fphar.2021.709702

Sample: serum
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
Comparison of Selenium Nanoparticles and Sodium Selenite on the Alleviation of Early Atherosclerosis by Inhibiting Endothelial Dysfunction and Inflammation in Apolipoprotein E-Deficient Mice

DOI: 10.3390/ijms222111612

PMID: 34769040
Sample: serum
Drug Design Development and Therapy (2022) IF: 4.319
The Preventive and Therapeutic Effects of Ajwa Date Fruit Extract Against Acute Diclofenac Toxicity-Induced Colopathy: An Experimental Study

DOI: 10.2147/DDDT.S344247

PMID: 35965961
Sample: serum
JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
Suhuang antitussive capsule ameliorates post-infectious cough in mice through AhR-Nrf2 pathway

DOI: 10.1016/j.jep.2021.114664

PMID: 34555451
Sample: lung tissue
Scientific Reports (2021) IF: 4.38
Effect of tiger milk mushroom (Lignosus rhinocerus) supplementation on respiratory health, immunity and antioxidant status: an open-label prospective study

DOI: 10.1038/s41598-021-91256-6

PMID: 34083710
Sample: saliva
BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
Marein ameliorates diabetic nephropathy by inhibiting renal sodium glucose transporter 2 and activating the AMPK signaling pathway in db/db mice and high glucose–treated HK-2 cells

DOI: 10.1016/j.biopha.2020.110684

Sample: serum
Food Quality and Safety (2022) IF: 3.102
Novel Functional Food from an invasive species Polygonum cuspidatum: Safety evaluation, Chemical Composition, and Hepatoprotective Effects

DOI: 10.1093/fqsafe/fyac032
Biomed Research International (2022) IF: 3.246
Glycine Improved Cryopreserved Spermatozoa Quality in Achai Bull

DOI: 10.1155/2022/8282387

PMID: 35968237
Sample: semen
Frontiers in Veterinary Science (2022) IF: 3.412
Ovarian Oxidative Stress Induced Follicle Depletion After Zona Pellucida 3 Vaccination Is Associated With Subfertility in BALB/c Mice

DOI: 10.3389/fvets.2022.814827

PMID: 35252419
Sample: Ovarian
PHARMACEUTICAL BIOLOGY (2022) IF: 3.503
Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway

DOI: 10.1080/13880209.2022.2070221

PMID: 35635784
Sample: brain tissue
Journal of Inorganic and Organometallic Polymers and Materials (2023) IF: 3.518
Preparation of Novel Composites of Polyvinyl Alcohol Containing Hesperidin Loaded ZnO Nanoparticles and Determination of Their Biological and Thermal Properties

DOI: 10.1007/s10904-023-02532-z
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2022) IF: 3.639
Flaxseed oil ameliorates aging in D-galactose induced rats via altering gut microbiota and mitigating oxidative damage

DOI: 10.1002/jsfa.12010

PMID: 35567370
Sample: serum,liver
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION (2022) IF: 3.682
Synergistic effect of ZnO nanoparticles and hesperidin on the antibacterial properties of chitosan

DOI: 10.1080/09205063.2022.2099668

PMID: 35797143
Sample: nanocomposites
International Journal of General Medicine (2023) IF: 2.145
Hematological, Inflammatory, Coagulation, and Oxidative/Antioxidant Biomarkers as Predictors for Severity and Mortality in COVID-19: A Prospective Cohort-Study

DOI: 10.2147/IJGM.S402206

Sample: Serum
WORK-A Journal of Prevention Assessment & Rehabilitation (2021) IF: 1.505
Evaluation of oxidative stress and pro-inflammatory cytokines in occupationally cadmium exposed workers

DOI: 10.3233/WOR-203302

PMID: 33104053
Sample: serum
JOURNAL OF OCCUPATIONAL AND ENVIRONMENTAL MEDICINE (2020) IF: 1.642
Spirometric Outcomes and Oxidative Stress Among Cement Factory Workers: Results From a Cross-Sectional Study

DOI: 10.1097/JOM.0000000000001991

PMID: 32796257
Sample: serum
Archives of Environmental & Occupational Health (2021) IF: 1.663
Selenium, oxidative stress and inflammatory markers in handicraft workers occupationally exposed to lead

DOI: 10.1080/19338244.2021.1968780

PMID: 34423749
Sample: serum
HIGH ALTITUDE MEDICINE & BIOLOGY (2022) IF: 1.981
A Nanocurcumin and Pyrroloquinoline Quinone Formulation Prevents Hypobaric Hypoxia-Induced Skeletal Muscle Atrophy by Modulating NF-κB Signaling Pathway

DOI: 10.1089/ham.2021.0127

PMID: 35384739
Sample: gastrocnemius,soleus muscles
International Journal of Food Science (2023)
Fabrication of Nutraceutical Beverage from Saffron (Crocus sativus L.) Extract and Studying Its Health Effects

DOI: 10.1155/2023/7130266

Sample: serum
Toxicology Reports (2019)
Elevated reproductive toxicity effects of diclofenac after withdrawal: Investigation of the therapeutic role of melatonin

DOI: 10.1016/j.toxrep.2019.06.009

Sample: plasma

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