Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (ABTS, Enzyme Method)
Price: $ 200
Price: $ 150
Size:
96T
48T
Quantity:
- Sample Type: serum;plasma;urine;saliva;tissue;cells
- Detection Instrument: Microplate reader(405-425 nm)
- Reasearch Areas: Oxidative stress;Plant Physiology Series
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For research use only. Order now, ship in 3 days
Product Details
Detection Principle | The principle of the ABTS method for determining the T-AOC is as follows. ABTS is oxidized to green ABTS+ by appropriate oxidant, which can be inhibited if there exist antioxidants. The T-AOC of the sample can be determined and calculated by measuring the absorbance of ABTS+ at 414 nm or 734 nm. Trolox is an analog of VE and has a similar antioxidant capacity to that of VE. Trolox is used as a reference for other antioxidant antioxidants. For example, the T-AOC of Trolox is 1, then the antioxidant capacity of the other substance with the same concentration is showed by the ratio of its antioxidant capacity to Trolox antioxidant capacity. | ||||||||||||||||||
Synonyms | T-AOC | ||||||||||||||||||
Sample Type | serum,plasma,urine,saliva,tissue,cells | ||||||||||||||||||
Sensitivity | 0.047 mmol/L | ||||||||||||||||||
Detection Range | 0.047-1.50 mmol/L | ||||||||||||||||||
Detection Method | Colorimetric method | ||||||||||||||||||
Assay Time | 20 min | ||||||||||||||||||
Precision | inter-assay CV: 4.1% | intra-assay CV: 2.2% | ||||||||||||||||||
Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4), 60% Ethanol | ||||||||||||||||||
Storage | This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. | ||||||||||||||||||
Valid Period | 12 months | ||||||||||||||||||
Dilution Of Sample |
The recommended dilution factor for different samples is as follows (for reference only):
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When the sample was water soluble, the diluent is PBS (0.01 M, pH 7.4); When the sample is insoluble, the diluent is 60% ethanol. For the dilution of other sample types, please do pretest to confirm the dilution factor. |
Citations
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The Anti-Inflammatory Effect of a Combination of Five Compounds From Five Chinese Herbal Medicines Used in the Treatment of COPD
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Journal:Scientific Reports
DOI:10.1038/s41598-021-91256-6
PMID:34083710
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Suhuang antitussive capsule ameliorates post-infectious cough in mice through AhR-Nrf2 pathway
IF:4.36
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PMID:34555451
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The Preventive and Therapeutic Effects of Ajwa Date Fruit Extract Against Acute Diclofenac Toxicity-Induced Colopathy: An Experimental Study
IF:4.319
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Fetal hyperhomocysteinemia is associated with placental inflammation and early breakdown of maternal-fetal tolerance in Pre-term birth
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Support for increased cardiovascular risk in non‐segmental vitiligo among Egyptians: A hospital‐based, case–control study
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