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Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (ABTS, Enzyme Method)

Cat:E-BC-K219-M Citations (32)
Manual MSDS

Price: $ 200

Price: $ 150

Size:
96T 48T
Quantity:
  • Sample Type: serum;plasma;urine;saliva;tissue;cells
  • Detection Instrument: Microplate reader(405-425 nm)
  • Reasearch Areas: Oxidative stress;Plant Physiology Series
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Product Details
Detection Principle The principle of the ABTS method for determining the T-AOC is as follows. ABTS is oxidized to green ABTS+ by appropriate oxidant, which can be inhibited if there exist antioxidants. The T-AOC of the sample can be determined and calculated by measuring the absorbance of ABTS+ at 414 nm or 734 nm. Trolox is an analog of VE and has a similar antioxidant capacity to that of VE. Trolox is used as a reference for other antioxidant antioxidants. For example, the T-AOC of Trolox is 1, then the antioxidant capacity of the other substance with the same concentration is showed by the ratio of its antioxidant capacity to Trolox antioxidant capacity.
Synonyms T-AOC
Sample Type serum,plasma,urine,saliva,tissue,cells
Sensitivity 0.047 mmol/L
Detection Range 0.047-1.50 mmol/L
Detection Method Colorimetric method
Assay Time 20 min
Precision inter-assay CV: 4.1% | intra-assay CV: 2.2%
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4), 60% Ethanol
Storage This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Valid Period 12 months
Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
10% Rat brain tissue homogenization 1
10% Rat liver tissue homogenization 1
10% Rat kidney tissue homogenization 1
10% Epipremnum aureum tissue homogenization 1
Human serum 1
Human saliva 1
Human urine 1
Rat serum 1
When the sample was water soluble, the diluent is PBS (0.01 M, pH 7.4); When the sample is insoluble, the diluent is 60% ethanol. For the dilution of other sample types, please do pretest to confirm the dilution factor.