Total Cholesterol (TC) Colorimetric Assay Kit (Single Reagent, COD-PAP Method)
Price: $ 500
Price: $ 180
Price: $ 120
Size:
500Assays
96T
48T
Quantity:
- Sample Type: serum;plasma;tissue
- Detection Instrument: Microplate reader(495-525 nm)
- Reasearch Areas: Metabolic Diseases;Glycolysis Lipid Metabolism
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Product Details
Detection Principle | Total cholesterol includes free cholesterol and cholesterol esters. Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce △4-cholestenone and hydrogen peroxide. In the presence of 4-aminoamylpyridine and phenol, hydrogen peroxide catalyze peroxidase to form red quinone compounds of benzoquinone imine phenizone.The color depth of the generated quinone is directly proportional to the cholesterol content. The absorbance values of the standard tube and the sample tube are measured respectively, and the cholesterol content in the sample can be calculated. | ||||||||||||||
Synonyms | TC | ||||||||||||||
Sample Type | serum,plasma,tissue | ||||||||||||||
Sensitivity | 0.29 mmol/L | ||||||||||||||
Detection Range | 0.29-25.85 mmol/L | ||||||||||||||
Detection Method | Colorimetric method | ||||||||||||||
Assay Time | 20 min | ||||||||||||||
Precision | inter-assay CV: 8.3% | intra-assay CV: 3.1% | ||||||||||||||
Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4), Anhydrous ethanol | ||||||||||||||
Storage | This product can be stored at 2~8°C for 12 months with shading light. | ||||||||||||||
Valid Period | 12 months | ||||||||||||||
Images | |||||||||||||||
Dilution Of Sample |
The recommended dilution factor for different samples is as follows (for reference only):
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The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) for serum (plasma) samples; The diluent is anhydrous ethanol for tissue samples;. For the dilution of other sample types, please do pretest to confirm the dilution factor. |
Citations
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