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Total Cholesterol (TC) Colorimetric Assay Kit (Single Reagent, COD-PAP Method)

Cat:E-BC-K109-M Citations (36)
Manual MSDS

Price: $ 500

Price: $ 180

Price: $ 120

Size:
500Assays 96T 48T
Quantity:
  • Sample Type: serum;plasma;tissue
  • Detection Instrument: Microplate reader(495-525 nm)
  • Reasearch Areas: Metabolic Diseases;Glycolysis Lipid Metabolism
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Product Details
Detection Principle Total cholesterol includes free cholesterol and cholesterol esters. Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce △4-cholestenone and hydrogen peroxide. In the presence of 4-aminoamylpyridine and phenol, hydrogen peroxide catalyze peroxidase to form red quinone compounds of benzoquinone imine phenizone.The color depth of the generated quinone is directly proportional to the cholesterol content. The absorbance values of the standard tube and the sample tube are measured respectively, and the cholesterol content in the sample can be calculated.
Synonyms TC
Sample Type serum,plasma,tissue
Sensitivity 0.29 mmol/L
Detection Range 0.29-25.85 mmol/L
Detection Method Colorimetric method
Assay Time 20 min
Precision inter-assay CV: 8.3% | intra-assay CV: 3.1%
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4), Anhydrous ethanol
Storage This product can be stored at 2~8°C for 12 months with shading light.
Valid Period 12 months
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Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Human serum 1
Mouse serum 1
Rat plasma 1
10% Mouse liver tissue homogenate 1
10% Mouse kidney tissue homogenate 1
10% Mouse heart tissue homogenate 1
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) for serum (plasma) samples; The diluent is anhydrous ethanol for tissue samples;. For the dilution of other sample types, please do pretest to confirm the dilution factor.