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Total Iron Binding Capacity (TIBC) Colorimetric Assay Kit

Cat:E-BC-K071-S Citations (2)
Manual MSDS

Price: $ 180

Size:
50Assays
Quantity:
  • Sample Type: serum
  • Detection Instrument: Spectrophotometer(520 nm)
  • Reasearch Areas: Inorganic ions
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Product Details
Detection Principle The excess iron is added to the serum to bind all the ferritin in the serum, and the excess iron is adsorbed by adding the iron adsorbent. The iron bind with the ferritin is separated from the protein by the action of acid solution and reductant. Fe3+ in serum is reduced to Fe2+, Fe2+ binds with bipyridine to form pink complex. In a certain range, the amount of TIBC is positively correlated with the depth of color. The iron content measured is, minus serum iron value, which is called unsaturated iron binding force. Total iron binding capacity minus serum iron value is unsaturated iron binding capacity (UIBC).
Synonyms TIBC
Sample Type serum
Sensitivity 0.03 mg/L
Detection Range 0.03-50 mg/L
Detection Method Colorimetric method
Assay Time 50 min
Precision inter-assay CV: 4.7% | intra-assay CV: 3.4%
Other Reagents Required Normal saline (0.9% NaCl)
Storage This product can be stored at 2~8°C for 12 months with shading light.
Valid Period 12 months
Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Human serum 1
Rat serum 1
Porcine serum 1
Rabbit serum 1
Chicken serum 1
Cynomolgus monkey serum 1
The diluent is double distilled water or normal saline (0.9% NaCl). For the dilution of other sample types, please do pretest to confirm the dilution factor.